Kidney stone is a complex disease resulting from environmental as well as hereditary factors and principally composes of approximately 75% calcium oxalate (CaOx) crystals, which are formed through a multi-step process. Osteopontin gene was identified by isolating cDNA from cultured rat osteosarcoma cells in 1986. Osteopontin gene located on human chromosome 4 (4q22.1). This gene encodes several non-collagenous bone and dentin proteins. In this study, 93 normal control samples and 92 kidney stonedisease samples that were grouped according to the types of kidney stone disease and clinical characteristics of patients, including gender and average age were observed with restriction fragment length polymorphism (RLFP) technique.58 patients and 43 controls displayed the “C or G” / “C or G” genotype, 48 patients and 34 controls displayed T / “G or C” genotype. Since there is no appropriate restriction enzyme to recognize the G or C nucleotide in that position, it could not be possible to discriminate the C-G nucleotides. Additionally no T/T genotype was observed. The results werefound statistically significant by chi-square test (p