Haematological Variations in Brucella abortus Antibody Positive Cross-bred Cattle at Chittagong, Bangladesh

Mayıs 2012 den Ocak ayına kadar Bangladeşin Chittagong bölgesinde bruselloz seropozitif bulunan ticari sağmal ineklerin kan parametrelerinde önemli farklılıkları belirlemek amacıyla hematolojik bir çalışma yürütüldü. 250 ticari melez süt sığırından oluşan çalışmada, rastgele 7 çiftlik seçildi. Bir tarama testi olarak Milk Ring Testi (MRT) yapıldı. MRTde pozitif sonuç veren 50 inekten hematolojik ve serolojik testler için kan alındı. Serumların ayrılmasından sonra, konfirmasyon amacıyla Enzyme Linked Immuno Sorbent Assay (iELISA) ve Rose Bengal Plate Testi (RBPT) yapıldı. Brusella pozitif ve negatif gruplar arasında anlamlı bir varyasyon mevcut olup olmadığını gözlemek için hematolojik testlerden hemoglobin (Hb), paketlenmiş hücre hacmi (PCV), eritrosit sedimantasyon oranı (ESR), kırmızı (TEC) ve beyaz (TLC) kan sayımı, diferansiyel lökosit sayısı (DLC), eritrosit hacmi (MCV), ortalama eritrosit hemoglobini (MCH) ve eritrosit hemoglobin konsantrasyonu (MCHC) belirlendi. Sonuçlar parametreler arasında anlamlı farklılıklar bulunmadığını gösterdi (p

Chittagong, Bangladeşte Brucella abortus Antikor Pozitif Melez Sığırlarda Hematolojik Varyasyonlar

A hematological study was carried out to determine the considerable variations in blood parameters for brucellosis seropositivity in commercial dairy cattle in the Chittagong region of Bangladesh from January to May 2012. The study population comprised of 250 commercial cross-breed dairy cattle, randomly selected from 7 commercial farms. Milk Ring Test (MRT) was done as a screening test. The MRT positive 50 cows were subjected to blood collection for hematological and serological tests. After separation of sera, two serological tests specifically indirect Enzyme Linked Immuno Sorbent Assay (iELISA) and Rose Bengal Plate Test (RBPT) were done for confirmation. Hematological tests like hemoglobin (Hb), packed cell volume (PCV), erythrocyte sedimentation rate (ESR), red (TEC) and white (TLC) blood cell count, differential leukocyte count (DLC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) were determined to observe whether any significant variation between the brucellosis positive and negative group was exist. The results revealed that no significant variations were found among the parameters (p<0.05). However, slightly increased values of TLC, monocytes, eosinophil, MCV and MCH were recorded in the positive group. In addition, a little decline in the values of TEC, and neutrophil were found in the same group. The values of Hb, PCV, ESR, lymphocytes, basophils and MCHC were remained unchanged. The results showed that Brucella organisms are not responsible for a significant change in the hematological values, underscoring the need for further studies including chemical and structural changes in the serum or tissue or cellular or molecular level.

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