Ahmet Metin KUMLAY, Serap DEMİREL, Fatih DEMİREL, Bünyamin YILDIRIM

Bazı Soya (Glycine max L.) Çeşitlerinin IPBS Markörleriyle Moleküler Karakterizasyonu

Küresel ısınma ve insan popülasyonunun sürekli artması besin kaynağı üzerinde baskı oluşturmaktadır. Soya fazlaca protein ve yağ içermesinden dolayı insan beslenmesinde önemli rol oynamaktadır. Bu çalışmada 12 soya çeşidinin (Nova, Nazlican, SA88, Ataem7, Arısoy, A3127, Türksoy, Adasoy, Yemsoy, ANP2018, Yeşilsoy ve Samsoy) 6 IPBS markörü ile genetik ilişkisi incelenmiştir. IPBS analizi sonucuna göre toplam 44 polimorfik bant elde edilmiş olup ortalama polimorfizm oranı %85.83 olmuştur. Primer başına elde edilen ortalama polimorfik bant sayısı 7.33 olarak belirlenmiştir. Geneotipler arasında Dice benzerlik indeksi 0.1 ile 0.9091 arasında değişmiş olup ortalama benzerlik indeksi 0.4506 olarak hesaplanmıştır. Analizlerin sonuçlarına göre, en yakın akrabalık gösteren genotiplerin Yeşilsoy ile Arısoy çeşitleri iken, en az benzeyen genotiplerin ise Samsoy ile Yemsoy çeşitleridir. Sonuç olarak, IPBS markörleri soya ıslahı programlarında uygun ebeveynlerin seçiminde genotipleri karakterize etmek için etkili bir şekilde kullanılabilir.

Anahtar Kelimeler:

Glycine max L., IPBS, Genetik ilişki, ıslah

Molecular Characterization of Some Soybean (Glycine max L.) Varieties

Global warming and the continuous increasing in human population have adverse effects on the food source. Soybean plays a significant role in human nutrition due to its high protein and fat content. In this study, the genetic relationship of 12 soybean varieties (Nova, Nazlican, SA88, Ataem7, Arısoy, A3127, Türksoy, Adasoy, Yemsoy, ANP2018, Yeşilsoy and Samsoy) was investigated using 6 IPBS markers. According to the results of the IPBS analysis, totally 44 polymorphic bands were obtained and the average polymorphism rate was 85.83%. The average number of polymorphic bands obtained per primer was determined as 7.33. The Dice similarity index between genotypes varied between 0.1 and 0.9091 with the average being 0.4506. According to the results of the analysis, the genotypes showing the closest relationship are Yeşilsoy and Arısoy varieties, while the farthest genotypes are Samsoy and Yemsoy. As a result, IPBS markers can be used effectively to characterize genotypes in the selection of suitable parents in soybean breeding programs.

Keywords:

Glycine max L., IPBS, Genetic diversity, breeding,

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