Akkaraman Koyun Irkında Callipyge Gen Polimorfizmin Araştırılması

Callipyge (CLPG) koyunlarda et kalitesi ve büyüme üzerinde önemli etkisi olan bir aday gendir. Çalışmada Akkaraman koyun ırkında CLPG gen polimorfizmin belirlemesi amaçlanmıştır. Araştırmada Akkaraman ırkı 50 baş koyuna ait kan örneklerinden elde edilen DNA’lar çalışma materyali olarak kullanılmıştır. Tüm bireylerde CLPG/FaqI polimorfizmi PCR-RLP yöntemi ile araştırılmıştır. PCR-RFLP işlemi sonrasında sadece A alleli ve AA genotipi tespit edilmiştir. Bu çalışma CLPG gen polimorfizmin Akkaraman ırkında araştırıldığı ilk çalışma olup elde edilen bulgular Akkaraman ırkının CLP/Faq1 polimorfizmi bakımından monomorfik bir yapıya sahip olduğunu göstermiştir.

Anahtar Kelimeler:

Akkaraman koyunu, Callipyge, FaqI, PCR-RFLP

Investigation of Callipyge Gene Polymorphism in Akkaraman Sheep Breed

Callipyge (CLPG) is a candidate gene with a significant effect on meat quality and growth in sheep. The aim of this study was to investigate the polymorphism of the CLPG gene in the Akkaraman breed. In the study, DNAs obtained from the blood samples of 50 Akkaraman sheep was used as study material. The CLPG/Faq1 polymorphism was examined in all individuals using the method PCR-RFLP. Only A allele and AA genotype were detected after PCR-RFLP procedure. This is the first study in which CLPG gene polymorphism was investigated in Akkaraman breed, and the findings showed that the Akkaraman breed has a monomorphic structure in terms of CLPG/FaqI polymorphism.

Keywords:

Akkaraman sheep, Callipyge, FaqI, PCR-RFLP,

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