Keratokonjunktivitisli bir tavuktan Corynebacterium spp. ve Arcanobacterium spp. izolasyonu

Bu çalışmada, Tekirdağ ilinde bir tavuk çiftliğinde saptanan Corynebacterium ve Arcanobacterium spp. enfeksiyon olgusu sunuldu. Tekirdağ Namık Kemal Üniversitesi Veteriner Fakültesi Mikrobiyoloji Anabilim Dalı Laboratuvarına gözde tek taraflı şiddetli keratokonjunktivitise bağlı kapanma, yüzde ödem ve solunum güçlüğü şikâyetiyle getirilen bir tavuğa nekropsi yapıldı. Tavuğun gözünden alınan irin ve diğer nekropsi materyallerinden (akciğer, kalp, karaciğer,dalak) uygun besi yerlerine ekimler yapıldı. İzole edilen bakterilerin identifikasyonu amacıyla rutin biyokimyasal testler uygulandı. Nekropsi makrsoskobik incelemede; gözün tamamen kapandığı ve içerisinin irinle dolu olduğu, akciğerde konjeste alanlar ve multifokal renk değişimleri ile kalpte hafif bir büyüme gözlendi. Materyallerden yapılan ekimlerde Columbia agar (%5 koyun kanlı) ve Tryptic Soy agarda üreme olurken, Mac Conkey ve Eosin Methylene Blue agarda üreme görülmedi. Karaciğer ve dalaktan yapılan ekimlerde üreme olmadı. Gram boyamada Gram pozitif çomaklar ve kokobasiller tespit edildi. Biyokimyasal testlerle; gözden alınan irinden Corynebacterium spp., akciğerden alınan örnekten Arcanobacterium spp. ve kalpten alınan örnekten Corynebacterium spp. izole ve identifiye edildi. İzole edilen bu etkenler kanatlı hayvan türleri için potansiyel hastalık riski oluşturması açısından kayda değer bulundu.

Anahtar Kelimeler:

Akciğer, Arcanobacterium spp., Corynebacterium spp., Göz, Tavuk

Isolation of Corynebacterium spp. and Arcanobacterium spp. from a chicken with keratoconjunctivitis

In this study, a case of Corynebacterium spp. and Arcanobacterium spp. infection is presented from a poultry farm in Tekirdağ city. Necropsy was performed on a chicken brought to Tekirdağ Namık Kemal University Veterinary Faculty Microbiology Department Laboratory with complaints of unilateral severe keratoconjunctivitis, facial oedema and respiratory distress. The pus from the eye of the chicken and other necropsy materials (lung, heart, liver, spleen) were inoculated into appropriate media. Routine biochemical tests were performed to isolate and identify the growing bacteria. In necropsy macroscopic examination, a slight enlargement of the heart was observed with congested areas and multifocal colour changes in the lung, in which the eye was completely closed and filled with pus. While bacterial growth was observed on Columbia agar (5% sheep blood) and Tryptic Soy agar, no growth was observed on Mac Conkey and Eosin Methylene Blue agar from inoculated samples. There was no bacterial growth in the liver and spleen samples. The Gram-positive rods and cocobacilli were detected with Gram staining. As a result of biochemical tests; Corynebacterium spp. and Arcanobacterium spp. from the lung sample, and Corynebacterium spp. from the pus sample of the eye were isolated and identified. These isolations were found to be significant in terms of potential disease risk for poultry species.

Keywords:

Arcanobacterium spp., Chicken, Corynebacterium spp., Eye, Lung,

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