Çiğ süt örneklerinden izole edilen Staphylococcus aureusizolatlarında antibiyotik direnç özelliklerinin tespiti

Staphylococcus aureusönemli bir gıda patojeni olup, tüm dünyada görülen gıda intoksikasyonları içerisinde üst sıralarda yer almaktadır. S. aureus’un gıda intoksikasyonlarında etkili olması yanında önemli bir diğer halk sağlığı problemi de farklı antibiyotiklere karşı şekillenen tekli veya çoklu antibiyotik direncidir. Bu çalışmada toplam 60 adet çiğ süt örneğinden izole edilen 31 S. aureusizolatı antibiyotik direnç özellikleri yönünden analiz edilmiştir. İzolatların antimikrobiyel duyarlılıkları disk difüzyon yöntemi ile belirlenmiştir. Bu analiz, CLSI (Clinical and Laboratory Standarts Institute) tarafından bildirilen yöntem esas alınarak, tetrasiklin,gentamisin, eritromisin, trimetoprim-sülfametaksazol, kloramfenikol, siprofloksasin ve klindamisin antibiyotik diskleri ile yapılmıştır. Analiz sonucunda gentamisin, eritromisin, tetrasiklin, siprofloksasin, klindamisin, trimetoprim-sülfametaksazol ve kloramfenikole karşı gelişen direnç sırasıyla %3,33, %6,46 , %16,12, %6,47 ,%80,64 ,%6,46 , %16,13 oranında tespit edilmiştir. Yetiştiriciler antibiyotik kullanımı konusunda bilinçlendirilmeli ve yasal bekleme sürelerine özen göstermelidir. Ayrıca yetkili otoriteler tarafından sütte antibiyotik varlığının tespitine yönelik kontroller düzenli olarak yapılmalıdır.

Detection of antibiotic resistance properties of Staphylococcus aureus isolated from raw milk samples

Staphylococcusaureusis an important food pathogen and it is the main food intoxication agent all over the world. Also, S. aureusmay cause a public health problem which is single or multiple antibiotic resistance against various antibiotics. In this study, thirty one S. aureusisolates which obtained from a total of 60 raw milk samples were analyzed for their antibiotic resistance properties. Antimicrobial susceptibilities of isolates were determined by disk diffusion methodwithtetracycline, gentamycin, erythromycin,trimethoprim-sulfamethoxazole, chloramfenicole, ciprofloxacin andclindamycindisksaccording to CLSI (Clinical and Laboratory Standarts Institute). Based on our results, antibiotic resistance of S. aureusisolates were detected in gentamycin, erythromycin, tetracycline, ciprofloxacin, clindamycin, trimethoprim-sulfamethoxazole and chloramfenicole as 3.33%, 6.46% , 16.12%, 6.47%, 80.64%, 6.46%, 16.13%, respectively. Dairy farmers should be educated aboutantibiotic use and comply withlegal waiting periods in milk productions.Also,antibiotic residues in milk should be controlled regularly by local authories.

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