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SALMONELLA CRISPR-Cas SİSTEMİ’NİN TEMEL ÖZELLİKLERİ

Son yıllarda keşfedilen CRISPR-Cas sistemi, CRISPR dizileri (düzenli aralıklarla bölünmüş palindromik tekrar kümeleri) ve Cas (CRISPR ilişkili proteinler) genlerinden oluşmaktadır. 1987 yılında bu tekrar kümeleri ilk olarak Escherichia coli’de keşfedilmiş ancak fonksiyonları tanımlanamamıştır. Günümüzde Salmonella da dahil olmak üzere bakteri genomlarının yaklaşık % 45'inde bulunan CRISPR-Cas sisteminin bakterilerin nükleik asit tabanlı adaptif bağışıklık sisteminin temel bileşenleri olduğu bilinmektedir. CRISPR-Cas bölgelerinin analizine dayalı çalışmaların son yıllarda oldukça artması, CRISPR tabanlı teknolojilerin ve uygulamaların çoğalması bu alanda yapılan çalışmaların etkinliğini de giderek artırmaktadır. Bu derlemede CRISPR-Cas sistemi ve Salmonella’da mevcut olan CRISPR bölge özellikleri ile kullanım alanları hakkında bilgi verilecektir
Anahtar Kelimeler:

CRISPR, Moleküler tiplendirme, Salmonella

The CRISPR-Cas system, discovered in recent years, consists of CRISPR sequences (clusters of regularly spaced palindromic repeats) and Cas (CRISPR-associated proteins) genes. These repeat clusters were first discovered in Escherichia coli in 1987, but their function has not been defined. It is known that the CRISPR-Cas system, which is present in approximately 45% of bacterial genomes, including Salmonella, is the essential component of the nucleic acid-based adaptive immune system of bacteria. The increase in studies based on the analysis of CRISPR-Cas regions and the proliferation of CRISPR-based technologies and applications increase the effectiveness of studies in this field. In this review, information will be given about the CRISPR-Cas system and the CRISPR region features and usage areas in Salmonella.
Keywords:

CRISPR, Molecular typing, Salmonella,

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Veteriner Farmakoloji ve Toksikoloji Derneği Bülteni-Cover
  • Başlangıç: 2010
  • Yayıncı: Veteriner Farmakoloji ve Toksikoloji Derneği
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