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Sıçanlarda Karbon Tetraklorürün (CCl4) Neden Olduğu Koagülasyon Bozukluklarında Etil Pirüvatın Etkisi

Çalışmada, sıçanlarda karbon tetraklorürün (CCl4) neden olduğu koagülasyon bozukluklarında etil pirüvatın (EP) etkisi araştırıldı. Kırk erkek Sprague Dawley ırkı sıçan dört eşit gruba ayrıldı. Kontrol grubuna 1 ml Ringer laktat solüsyonu 0., 1,5. ve 6. saatlerde periton içi verildi. Karbon tetraklorür grubuna 1.6 g/kg CCl4 periton içi yolla tek doz verilmesini takiben 1.5 ve 6 saat sonra 1 ml Ringer laktat uygulandı. Etil pirüvat grubuna 40 mg/kg dozda etil pirüvat 0, 1.5 ve 6. saatlerde periton içi enjekte edildi. Etil pirüvat+CCl4 grubuna ise tek doz 1.6 g/kg CCl4 uygulamasından 30 dak. önce ve 1 ve 6 saat sonra 40 mg/kg dozda etil pirüvat periton içi uygulandı. Karbon tetraklorür, protrombin zamanı (PTZ) ve aktive parsiyel tromboplastin zamanı (aPTT) değerlerinde uzama (P<0.05), fibrinojen düzeyinde azalma (P<0.05) ve alanin aminotransferaz (ALT) aktivitesinde artma (P<0.05) oluşturdu. Öte yandan, CCl4 grubuyla karşılaştırıldığında, CCl4 uygulamasından 30 dak. önce ve 1 ve 6 saat sonra 40 mg/kg dozda verilen etil pirüvat fibrinojen düzeyini artırırken (P<0.05) PTZ ve aPTT sürelerinde ve ALT aktivitesinde azalmaya (P<0.05) neden oldu. Sonuç olarak, etil pirüvatın sıçanlarda CCl4’ün neden olduğu pıhtılaşma bozuklukları üzerine koruyucu bir etki gösterebileceği tespit edildi. Etil pirüvatın bu etkisi, karaciğer hastalıklarıyla ilişkili pıhtılaşma bozukluklarının önlenmesinde yararlı olabilir.

Anahtar Kelimeler:

Etil pirüvat, Karbon tetraklorür, Pıhtılaşma bozuklukları

Effect of Ethyl Pyruvate on Carbon Tetrachloride (CCl4)-Induced Coagulation Disturbances in Rats

The effect of ethyl pyruvate (EP) on carbon tetrachloride (CCl4)-induced haemostatic disturbances in rats was investigated in this study. Forty male Sprague-Dawley rats were divided into four equal groups. The control group was given intraperitoneally 1 ml of Ringer’s lactate solution at 0 h, 1,5 h and 6 hours. The CCl4 group was treated intraperitoneally with a single dose of 1.6 g/kg CCl4 followed by the administration of 1 ml Ringer’s lactate solution at at 1,5 and 6 hours. The ethyl pyruvate group was injected intraperitoneally with ethyl pyruvate at a dose of 40 mg/kg at 0, 1,5 and 6 hours. The ethyl pyruvate +CCl4 group were administered intraperitoneally with ethyl pyruvate at a dose of 40 mg/kg at 30 min before and at 1 and 6 h after a single dose of 1.6 g/kg CCl4 injection.Treatment of CCl4 prolonged prothrombin time (PT) and activated thromboplastin time (aPTT), decreased fibrinogen level and increased alanine aminotranspherase (ALT) activity. On the other hand, the administration of EP intraperitoneally at 30 minutes before, and at 1.5 and 6 h after CCl4 significantly (P<0.05) decreased PT, aPTT and ALT values and increased (P<0.05) fibrinogen level when compared with CCl4-treated only group. The results of our study suggest that ethyl pyruvate treatment plays a protective role on CCl4-induced coagulation disturbances in rats. This effect of ethyl pyruvate may be useful for preventing haemostatic disturbances associated with liver diseases

Keywords:

Carbon tetrachloride, Coagulation disorders, Ethyl pyruvate,

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