Radyasyona Maruz Kalan Ratlarda Oksidatif Stres Üzerine Resveratrolün Etkisi

Radyasyonun yarattığı oksidatif hasar üzerine resveratrolün antioksidan ve koruyucu etkisi araştırıldı. Yirmi bir rat 3 gruba ayrıldı; Birinci grup, Kontrol grubu. İkinci grup, Radyasyon grubu; Üç gün fizyolojik tuz solüsyonu intraperitoneal (i.p.) olarak verildi ve sonra 9 Gray (Gy) radyasyon uygulandı. Üçüncü grup, Radyasyon + Resveratrol grubu; Üç gün 10 mg/kg olacak şekilde resveratrol i.p. verildi ve sonra aynı dozda radyasyon uygulandı. Radyasyon sonucu ratların karaciğer, böbrek ve beyin dokularında malondialdehit (MDA) seviyesinde önemli artış saptandı (P˂0.001). Çalışılan tüm dokularda glutatyon peroksidaz (GSH-Px) aktivitesinde (P˂0.01), yine karaciğer süperoksit dismutaz (SOD) aktivitesi ve glutatyon (GSH) seviyesinde azalış (P˂0.01), böbrek, dalak ve ovarium dokularında GSH düzeyinde ise önemli artış tespit edildi (P˂0.05). Resveratrol verilmesi radyasyon sonucu karaciğer, böbrek ve beyinde artan MDA düzeylerini önemli azalttı (P˂0.001) ve karaciğer MDA düzeyleri kontrol düzeylerine ulaştı. Resveratrol verilmesiyle çalışılan tüm antioksidanların kontrol ve/veya radyasyon uygulanan gruba göre önemli azalma gösterdiği tespit edildi (P˂0.05). Sonuç olarak elde edilen verilere göre; resveratrolün radyasyona bağlı oluşan oksidatif strese karşı oksidan/antioksidan dengeyi değiştirerek düzeltici bir etki gösterdiği, bu nedenle resveratrolün güçlü bir antioksidan olduğu kanaatine varılmıştır. 

The Effect of Resveratrol on The Oxidative Stress in Rats Treated with Irradiation

The antioxidant and protective effect of resveratrol against irradiation induced oxidative damage were researched. Twenty-one rats were seperated into 3 groups; First group was the control group. Second group was the irradiation group; received physiological saline intraperitoneally (i.p.) for three days, then 9 Gray (Gy) irradiation was applied. Third group was irradiation+Resveratrol group; received 10 mg/kg resveratrol i.p. for three days and then the same dose of radiation was applied. As a result of irradiation, a significant increase was found in the level of malondialdehyde (MDA) in the liver, kidney and brain tissues of rats (P˂0.001). Glutathione peroxidase (GSH-Px) activity in all the tissues and superoxide dismutase (SOD) activity, Glutathione (GSH) levels in liver decreased (P˂0.01), GSH levels in kidney, spleen and ovarium tissues significantly increased (P˂0.05). The giving of resveratrol significantly reduced the MDA levels increasing in liver, kidney, and brain as a result of irradiation (P˂0.001) and the liver MDA levels reached to the control levels. By giving resveratrol, it was determined that all the antioxidants studied reduced significantly compared to the control group and/or the irradiation group (P˂0.05). According to the data obtained as a result; It was concluded that resveratrol shows a corrective effect by changing the oxidant/antioxidant balance against oxidative stress caused by irradiation, therefore resveratrol is a powerful antioxidant.

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