Koyun Babeziozisinde Plazma Arginaz, Gelsolin ve Sistatin C Arasında Olası Potansiyel Biyobelirteç Tayini: Parazitemi Oranına Göre

Bu çalışmanın amacı, koyun babeziosisinde arginaz aktivitesi, gelsolin (GLS) ve sistatin c (Cys c) seviyelerini ve potansiyel biyobelirteç tespitini incelemektir. Patojen kan parazitleri olan Babesia türleri, hayvancılık yönetiminde ekonomik sorunlara neden olur. Yayımlanan birçok çalışmada babeziosis kaynaklı biyokimyasal parametreler önerilmiş, ancak hiç kimse bu parametrelerdeki duyarlılık ve özgüllüğü ve değişiklikleri açıklığa kavuşturmamıştır. Akut babeziosisli koyunlar, kırmızı kan hücrelerinde Piroplazma formlarının görülmesi ve klinik bulgulara göre belirlendi. Yirmibeş adet enfekte ve aynı sayıda sağlıklı koyunlardan alınan kan örneklemesinden sonra, yukardaki parametreler plazmada ölçüldü. Sonuçlar, arginaz ve Cys c'de anlamlı bir artış (P<0.01) ve sağlıklı gruba kıyasla farklı parazitemi oranlarında GLS seviyelerinde anlamlı düşüş (P<0.01) gösterdi. Sonuç olarak, koyun babeziosis'inin özellikle böbreklerde (Cys c'nin yüksek olması nedeniyle) hücresel hasara neden olduğunu ve yüksek arginaz aktivitesinin Babesia ovis proliferasyon sürecinde önemli bir faktör olarak olarak göz önünde bulundurulabileceğini göstermiştir. Son olarak, GLS 'den farklı parazitemi oranlarında CYS c ve arginaz 'nin yüksek duyarlılığı, koyun Babeziosis 'in potansiyel biyomarkeri olarak göz önünde bulundurulabilir.

Determination of Possible Potential Biomarker Between Plasma Arginase, Gelsolin and Cystatin C in Sheep Babesiosis: Based on Parasitemia Rate

The aim of this study was to assay arginase activity, gelsolin (GLS) and cystatin c (Cys c) levels and determination of potential biomarker in sheep babesiosis. The Babesia genus as pathogen blood parasites causes economic problems in livestock management. Many published studies have suggested babesiosis-induced biochemical parameters, but no one have determined those parameters alterations and clarification of sensitivity and specificity. Sheep with acute babesiosis were identified based on clinical signs and the observation of Piroplasm forms in red blood cells. After blood sampling from 25 infected and same number healthy sheep, above parameters measured in plasma. The results indicated significant increase (P<0.01) in arginase and Cys c and significant decreases (P<0.01) in the levels of GLS in different parasitemia rates compared with healthy group. In conclusion, the results suggested that sheep babesiosis causes cellular damage especially in kidney (owing to high level of Cys c) and high arginase activity can be considered as one of essential factor in Babesia ovis proliferation process. Finally, high sensitivity of Cys c and arginase in the different parasitemia rates than GLS could be clarified as potential biomarker of sheep babesiosis.

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Veterinary Sciences and Practices-Cover
  • Başlangıç: 2022
  • Yayıncı: Atatürk Üniversitesi
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