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Gossypin’in Kato-III Hücrelerindeki Anti-Proliferatif ve Apoptotik Etkilerinin Belirlenmesi

Gastrik karsinoma yüksek morbidite ve mortalite ile insanlarda ve hayvanlarda görülen bir kanser türüdür. Gossypin Hibiscus vitifolius'tan izole edilen doğal bir flavonoid grubunda doğal bir maddedir. Bu çalışmada gossypin’in Kato-III gastrik karsinoma hücrelerinde anti-proliferatif ve apoptotik etkilerinin araştırılması amaçlanmıştır. Kato-III hücreleri uygun ortamlarda kültüre edildi ve gossypin’in etkin doz aralığı (5-100 μg/ml) ve hücre canlılığı üzerindeki etkileri MTT testi ile 24, 48 ve 72. saatlerde tespit edildi. RT-PCR yöntemi ile kaspaz-3, 9 ve NF-kB gen ekspresyonları belirlendi. Floresan boyama tekniği ile apoptoz seviyeleri tespit edildi. Sisplatin pozitif kontrol grubu olarak deney eklendi. Gossypin’in Kato-III hücre proliferasyonunu doza ve zamana bağlı olarak azalttığı tespit edildi. Gossypin kaspaz-3 ve kaspaz-9 seviyelerini artırırken NF-kB seviyelerini inhibe ettiği ve sonuçların sisplatin ile benzerlik gösterdiği tespit edildi. Hücre morfolojik analiz ve floresan boyama sonuçlarına göre gossypin 100 μg/ml dozunda apoptozu en fazla artıran grup olduğu gösterilmiştir. Sonuç olarak gossypin’in gastrik karsinoma hücrelerinde apoptozu aktive ettiği ve tedavide potansiyel bir aday olabileceği ortaya konmuştur.

Anahtar Kelimeler:

Apoptoz, Gossypin, Kaspaz, Kato-III

Investigation of Anti-Proliferative and Apoptotic Effects of Gossypin in Kato-III Cells

Gastric carcinoma is a type of cancer in humans and animals with high morbidity and mortality. Gossypin is a natural flavonoid group isolated from Hibiscus vitifolius. In this study, it was aimed to investigate the anti-proliferative and apoptotic effects of gossypin in Kato-III cells. Cells were cultured in standard culture conditions. Cell viability and the effective dose range (5-100 μg/ml) of gossypin were determined at 24, 48 and 72nd hours by MTT. Caspase-3, 9, and NF-kB gene expressions were determined by Real Time-PCR method. Apoptosis levels were determined by fluorescent staining method. Cisplatin was used as positive control group. It was determined that Gossypin decreased Kato-III cell proliferation depending on the dose and time. Gossypin was found to increase caspase-3 and caspase-9 levels while inhibiting NF-kB levels, and the results were similar to cisplatin. Fluorescent staining results showed that gossypin increased apoptosis. As a result, it has been revealed that gossypin activates apoptosis in gastric carcinoma cells and may be a potential candidate for treatment.

Keywords:

Apoptosis, Caspase, Gossypin, Kato-III,

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