Diş Dolgu Materyallerinin Karaciğer ve Böbrek Dokuları Üzerine Olan Zararlı Etkileri

Bu çalışmanın amacı restoratif dental materyallerin, sıçan böbrek ve karaciğer dokuları üzerindeki olası etkilerinin incelenmesidir. 32 adet, yetişkin, dişi Wistar albino cinsi ratlar Kontrol grubu, Dental amalgam grubu, Rezin kompozit grubu, Cam iyonomer grubu olmak üzere 4 gruba ayrılmış (n=8) ve ense kısımlarına yapılan kesilerle dental materyaller yerleştirilip 8 hafta beklenmiştir. Ötenaziden sonra yapılan nekropsi ile karaciğer ve böbrek dokuları alınmıştır. Kullanılan boyama yöntemleri, NF-κB p65 ve Hematoksilen&Eozin boyamadır. Biyokimyasal analiz olarak ise glutatyon, süper oksit dismutaz, lipit peroksidasyon ve katalaz seviyeleri ölçülmüştür. İmmün-pozitif hücreler, kontrol grubunda deney gruplarına göre daha az yoğunluk göstermiştir. Bununla birlikte, tüm deney gruplarında renal ve hepatik dejenerasyonlar görülmüştür. Tüm biyokimyasal analiz sonuçları, kontrol grubu hariç deney gruplarında oksidatif stres oluştuğunu göstermektedir. Çalışmamızda elde ettiğimiz sonuçlar, deney gruplarında kullanılan tüm restoratif dental materyallerin böbrek ve karaciğer dokusunda sitotoksik etki yarattığını göstermektedir. Çalışmamızda kullanılan restoratif dental materyaller, tüm deney gruplarında böbrek ve karaciğer dokularında oksidatif stres oluşmasıyla ilişkilendirilebilir

Anahtar Kelimeler:

Böbrek hasarı, Cam iyonomer, Dental amalgam, Karaciğer hasarı, Rezin kompozit

The Harmful Effects of Restorative Dental Filling Materials on The Kidney and Liver Tissues

The purpose of this study was to examine the potential effects of restorative dental materials on the kidneys and liver tissues of rats. The histological, immunohistochemical and biochemical analyzes were conducted using 32 adult female Wistar albino rats classified into 4 groups (n=8): Control group, Dental amalgam group, Resin composite group, and Glass-ionomer group. The dental materials mentioned were put on the backs of the rat heads and kept there for 8 weeks. After humanely euthanizing rats, kidney and liver tissue was removed by necropsy, and Hematoxylin&Eosin and NF-κB p65 staining methods were used. In addition, glutathione, superoxide dismutase, lipid peroxidation and catalase levels have been identified. Immune-positive cells were higher in all sample groups than in the control group. Renal and hepatic degeneration has also been found in dental filling content groups. All biochemical findings revealed oxidative stress in all groups with the exception for control group. The three dental filling products have significant cytotoxic effects on the kidney and liver tissues, and may have been linked with oxidative stress in the tissues of the kidney and liver.

Keywords:

Dental amalgam, Glass ionomer, Kidney damage, Liver damage, Resin composite,

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