Gebelik Süresince Aroclor 1254 (2 mg/kg/gün)’e Maruz Kalan Gebe Ratların Böbrek Beyin ve Karaciğer Dokularında Oksidant/Antioksidant Dengedeki Değişimler

Bu çalışma, Aroclor 1254’e maruz kalan gebe ratların beyin, böbrek ve karaciğer dokularında hem oksidant hem de antioksidant parametreleri araştırmak amacıyla yapılmıştır. Çalışmada ratlar iki gruba ayrılmıştır ve her bir grupta 10 rat bulunmaktadır. Birinci grup, gebe kontrol grubu ve ikinci grup, gebe Aroclor 1254 (2 mg/kg/gün, Aroclor 1254’ün bu dozu 20 gün boyunca subkutan olarak uygulandı). Her grupta yer alan gebe ratlar eter anestezi yöntemi ile uyutulmuş ve karaciğer, beyin ve böbrek dokuları çıkarılmıştır. Bu dokularda lipit peroksidasyon parametresi malondialdehid (MDA), glutatyon (GSH) seviyeleri ile glutatyon peroksidaz (GSH-Px), katalaz (CAT), süperoksit dismutaz (SOD), glutatyon redüktaz (GR), glutatyon S transferaz (GST) aktiviteleri ölçülmüştür. Çalışma sonucunda karaciğer, böbrek ve beyin dokularında analizi yapılan parametreler açısından iki grup arasında istatistiksel açıdan bir anlamlı bir fark tespit edilememiştir. Yapılan çalışma ile gebelik süresince uygulanan Aroclor 1254’ün (2 mg/kg) bir kısmının plasenta yoluyla yavruya geçmesi nedeniyle gebe ratın vücudunda kalan Aroclor 1254’ün miktarının gebe ratda oksidatif hasar oluşturabilecek kadar etkili olmadığı sonucuna varılmıştır. Özellikle yüksek dozda Aroclor 1254’ün gebe ratlara uygulandığı ileri bir çalışma ile; gebe ratlarda oluşabilecek oksidatif hasarın tespitinin yapılabileceği düşünülmektedir

Anahtar Kelimeler:

Poliklorin bifenil, Oksidatif stres, Gebelik, Antioksidan, Aroclor

Changes of the Oxidant/Antioxidant Equilibrium in Liver, Brain and Kidney Tissues of Pregnant Rats Exposed to Aroclor1254 (2mg/kg/day) During Pregnancy

This study was conducted in order to investigate both oxidant and antioxidant parameters in liver, kidney, and brain tissues of pregnant rats exposed to Aroclor 1254. In the study, rats were divided into two groups, each of which consisted of 10 rats. The first group was the pregnant control group, and the second group was the pregnant Aroclor 1254 (2 mg/kg/day dose of Aroclor 1254 was administered by subcutaneous injection for 20 days). The pregnant rats in each group were anesthetized by using ether anaesthesia method, and their liver, brain, and kidney tissues were resected. Parameter of lipid peroxidation malondialdehyde (MDA), glutathione (GSH) levels as well as glutathione peroxydase (GSH-Px), catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR), glutathione S- transferase (GST) activities were measured in these tissues. As a result of the study, no statistically significant difference was determined between two groups in terms of parameters analysed in liver, kidney, and brain tissues. This study concluded that since a part of Aroclor 1254 (2 mg/kg), which was administered during pregnancy, was transmitted to the infant via placenta, the amount of Aroclor 1254, left in the body of pregnant rat, was not so effective to create oxidative damage in the pregnant rat. It is thought that the oxidative damage which could occur in the pregnant rats could be determined particularly through an advanced study in which a high dosage of Aroclor 1254 is administered to the pregnant rats

Keywords:

Polychlorinated biphenyls, Oxidative stress, Pregnancy, Antioxidant, Aroclor,

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