Adem KABASAKAL, Ekrem DÜNDAR, Cemal ÜN, Kamil SEYREK

Boz Irk Sığırlarda Süt Verimi İle İlişkilendirilen Kappa-kazein (κ-kazein) Geninin Analizi

Bu çalışmada, Boz ırk sığırlarda süt verimi ile ilişkilendirilen kappa-kazein geninin (κ-kazein) analizi yapılmıştır. Çalışma kapsamında, Bandırma Koyunculuk Araştırma İstasyonunda bulunan 88 adet saf Boz ırk sığırdan kan örnekleri alındı. Alınan kan örneklerinden genomik DNA izole edildi. Daha sonra Polimeraz Zincir Reaksiyonu (PZR) kullanılarak her bir genomik DNA örneğinden κ-kazein fragmanları çoğaltıldı. Elde edilen bu fragmentler dizilendi. Dizilerin analizi sonucunda, κ-kazein geni için A, A1, B, G2 ve H allelleri tespit edildi. Yüksek süt evrimi ile ilişkilendirilen κ-kazein geninin B allelinin Boz ırk sığırlarda (0.23) düşük olduğu tespit edildi. κ-kazein genin A1 ve H allelleri Bos indicus’ta ve G2 alleli ise Bos grunniens’te tanımlanmıştır. Bu durum, Boz ırk (Bos taurus) sığırların Bos indicus ve Bos grunniens sığırlarla bir bağlantısının olduğunu göstermektedir.

Anahtar Kelimeler:

Boz ırk sığır, Kappa-kazein geninin (κ-kazein), Allel ve genotip frekansı

Analysis of Kappa-casein (κ-casein) Gene of Associated with Milk Yield on Turkish Grey Cattle Breed

In the present study, kappa-casein (κ-casein) gene related to milk yield were analysed in Turkish Grey steppe Grey cattle breeds. The blood samples used in the study were taken from 88 pure Turkish Grey steppe cattle breed at the Bandirma Sheep Research Station. Genomic DNA was isolated from blood samples taken. Polymerase chain reaction (PCR) was employed to amplify the κ-casein fragments from each genomic DNA sample. These fragments obtained were sequenced. Sequence analyses proved that there are A1, B, G2 and H alleles of κ-casein gene in Grey cattle breeds B allele frequency correlated with high milk yields of κ-casein gene (0.23) was relatively low in Grey cattle breed. A1 and H alleles of bovine κ-casein gene detected here were defined in Bos indicus and G2 allel of bovine κ-casein gene also determined in this study was reported to be found in Bos grunniens. These results indicate that there may be a relationship between Grey cattle breed (Bos taurus) and both Bos indicus and Bos grunniens

Keywords:

Turkish Grey steppe cattle breed, Kappa-casein gene (κ-casein), Allele and genotype frequency,

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