Ayşe USTA, Ahmet Cihat ÖNER, Veysel YÜKSEK, Semiha DEDE, Sedat ÇETİN

Deneysel Florozis Oluşturulmuş Ratların Böbrek Dokusunda C ve E Vitaminlerinin İnflamasyon Gen Ekspresyonu Üzerine Etkisi

Amaç: İnflamasyon, florozis toksikasyon mekanizmalarının aydınlatılmasında önemli bir adımdır. Antioksidan C ve E vitaminlerinin, floroziste tedavi amacıyla uygulanmasının, inflamasyon gen ekspresyonları üzerine olası etkilerinin ortaya konulması amacıyla bu çalışma planlanmıştır. Materyal ve Metod: Çalışmada 48 adet 200–250 g ağırlığında Wistar-Albino ırkı erkek rat kullanıldı. Kontrol(K), Mısır yağı(M), NaF(N), NaF+vitamin E(NVE), NaF+vitamin C(NVC), NaF+vitamin C+vitamin E(NVCE) olarak her biri 8 rattan oluşan 6 grup kullanıldı. 16 hafta NaF hayvanların içme sularına eklenerek 150 mg/kg olarak verildi. Vitamin E mısır yağında eritilerek verildiği için mısır yağı kontrol grubu oluşturuldu ve ratlara 0,2 ml/gün olarak mısır yağı verildi. Tedavi gruplarına 16 hafta NaF verildikten sonra 4 hafta vitamin C(100 mg/kg), vitamin E(300 mg/kg), vitamin C+vitamin E(100 mg/kg+300 mg/kg) oral olarak uygulandı. Deney sonunda tüm gruplarda rat böbrek dokusundan elde edilen RNA izolasyon ürünlerinde, inflamasyon markırlarından IL1-β, TNF-α genlerinin ekspresyonu real time-qPCR ile belirlendi. İnternal kontrol geni olarak beta-aktin (Actb) kullanıldı. Bulgular: TNF-α ekspresyon seviyeleri, en yüksek NaF grubunda bulundu. Diğer tüm gruplarda önemli oranda düşüktü. IL-1 β gen ekspresyon düzeylerinin Mısır yağı ve NaF+vitamin C+E gruplarında en düşük bulundu. NaF ve NaF+vitamin C gruplarında en yüksek bulundu. Sonuç: NaF ile deneysel oluşturulan floroziste artan florürün oluşturduğu olası böbrek hasarında tedavi amaçlı antioksidan vitamin uygulamasının inflamasyonu azalttığı görüldü. Vitamin C, vitamin E, vitamin C+vitamin E’nin inflamasyon markırları üzerinde kayda değer yararlı etkilerinin olduğu tespit edildi. NaF+vitamin C, NaF+vitamin E verilen gruplara kıyasla vitamin C+E kombinasyonunun inflamasyonu düşürmede daha etkili olduğu görüldü.

Anahtar Kelimeler:

Antioksidan vitamin, böbrek, florozis, gen ekpresyonu, inflamasyon

The Effect of Vitamins C and E on Inflammation Gene Expression in Experimental Fluorosis-Induced Rat Kidney Tissue

Objectives: Inflammation is an important step in elucidating fluorosis toxicity mechanisms. This study was planned in order to reveal the possible effects of the application of antioxidant vitamins C and E for the treatment of fluorosis on inflammation gene expressions. Material and Methods: In the study, 48 male Wistar-Albino rats weighing 200-250 g were used. Six groups of 8 rats each were used as control(K), Corn oil(M), NaF(N), NaF+vitamin E(NVE), NaF+vitamin C(NVC), NaF+vitamin C+vitamin E(NVCE). NaF was added to the drinking water of the animals for 16 weeks and given as 150 mg / kg. Since vitamin E was dissolved in corn oil, the corn oil control group was formed and Corn oil was given to rats 0.2 ml/day. After NaF was administration to treatment groups16 weeks, vitamin C (100mg/kg), vitamin E (300mg/kg), vitamin C+vitamin E (100 mg/kg+300mg/kg) were administered orally for 4 weeks. At the end of the experiment, the expression of inflammation markers IL1-β, TNF-α genes in RNA isolation products obtained from rat liver tissue in all groups were determined by real time-qPCR. Beta-actin (Actb) was used as the internal control gene. Results: TNF-α expression levels were highest in NaF group. It was significantly lower in all other groups. IL-1 β gene expression levels were the lowest in Corn oil and NaF+vitamin C+E groups. It was found the highest in NaF and NaF+vitamin C groups. Conclusion: In possible kidney damage caused by increased fluoride in the experimentally induced fluorosis with NaF, it was observed that the application of antioxidant vitamins for therapeutic purposes reduced inflammation. It was determined that vitamin C, vitamin E, vitamin C+vitamin E had significant beneficial effects on inflammation markers. It was observed that the combination of vitaminC+E was more effective in reducing inflammation compared to the groups given NaF+vitamin C and NaF+vitamin E.

Keywords:

Antioxidant vitamins, fluorosis, gene expression, inflammation, kidney,

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