Parameters as a fuel economy, exhaust emissions, enough yield taking from using alternative fuels in vehicles are dependent directly properties of using alternative fuel. Therefore, properties of using alternative fuels need to be close or suitable to those of self fuels in vehicles. So, properties of alternative fuels need to be determined. Right of these determines need to be done in the boundary of measurement where its standard is obvious. These measurements pose problems for fuel producers due to being time consuming, being very expensive, exhibiting sensitivity and requiring special and trained personal. In this study, in order to support the solution of the above mentioned problems, relations between the properties of the fuel have been determined henceforth it has been aimed determination of other properties via using these relations. For this aim Cotton oil ME, Hazelnut oil ME, Mustard ME, Palm ME, Canola ME, Soybean ME, Olive oil ME, Sunflower ME, Frying waste oil ME, Serving as food waste oil ME, Used olive oil ME, Used canola ME, Canola ME were produced and their properties were determined. Also by using literature data of Peanut ME, Babassu ME, Suet ME, Rubber seed ME, Karanja oil ME, Mahua oil ME, Nahor oil ME and Almond oil ME statistic relations between methyl esters fuel properties were statistically determined