Alaattin Özen, Ebru Taştekin, Suat Cakina, Sule Parlar, Nukhet Kurkcu, Necdet Sut, Cem Uzal

İyonizer Radyasyonun Neden Olduğu Akut Renal Hasara Karşı Melatonin ve Amifostinin Koruyucu Etkilerinin Karşılaştırılması

Bu çalışmanın amacı, erken dönemde iyonizer radyasyonun neden olduğu akut böbrek hasarı üzerine melatonin ve amifostinin koruyucu etkisinin histopatolojik olarak karşılaştırılmasıdır. 50 dişi albino rat kontrol, yalnız radyoterapi, radyoterapi+amifostin, radyoterapi+melatonin ve radyoterapi+amifostin+melatonin olmak üzere beş gruba ayrıldı. İntraperitoneal amifostin (200 mg/kg) ve intraperitoneal melatonin (10 mg/kg) radyoterapiden 30 dakika önce uygulandı. Tek tek ratların tüm vücutlarına 8 Gy eksternal radyopterapi uygulandı. Takip süresinin sonunda her bir hayvan için segmental veya total nekroz gibi böbrek korteksi glomerul hasarı sayılarak hasarlı glomerul yüzdesi tespit edildi. Amifostin, melatonin ve amifostin+melatonin'in koruyucu etkisi istatistiksel olarak gösterilmekle birlikte melatonin lehine bir avantaj tespit edilmiştir (p = 0.005). Melatonin’e amifostin eklenmesinin tek basına melatonine göre üstünlüğü olmamasına karşın (p = 0.243) amifostine melatonin eklenmesinin tek basına amifostine göre istatistiksel üstünlüğü gösterilmiştir (p = 0.003). Sonuç olarak melatonin’in iyonizer radyasyonun neden olduğu akut böbrek hasarı üzerine koruyucu etkisi amifostin’den histopatolojik olarak daha iyidir.

Anahtar Kelimeler:

Amifostin, melatonin, akut renal hasar, iyonizer radyasyon

Comparison of Protective Effect of Melatonin and Amifostine on Acute Renal Damage Caused by Ionizing Radiation

The aim of this study is to compare histopathologically the protective effect of melatonin and amifostine on radiation induced renal damage. 50 female albino rats were divided into five groups: control, radiotherapy alone, radiotherapy+amifostine, radiotherapy+melatonin, radiotherapy+amifostine+melatonin. Intraperitoneal amifostine (200 mg/kg) and intraperitoneal melatonin (10 mg/kg) was administered to 30 minutes before irradiation. Rats were irradiated with a single dose of 8 Gy on whole body. At the end of the follow-up period, percentage of damaged glomeruli was determined by counting damaged glomeruli of kidney cortex as segmental or total necrosis for each animal. The protective effect of each agent has been shown, and there is an advantage in favor of melatonin (p = 0.005). Although there is not an advantage of adding amifostine to melatonin (p = 0.243), there is statistically significant better protective effect in amifostine+melatonin group when compared with amifostine alone (p = 0.003). The protective effect of melatonin on radiation induced acute renal toxicity is histopathologically better than amifostine.

Keywords:

Amifostine, melatonin, acute renal damage, ionizing radiation,

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