Arzu AKPINAR-BAYİZİT, Eli̇f YILDIZ, Gülşah OKUMUŞ

Doğal Antioksidan Bileşikler: Nar Yan Ürünlerinin Antioksidan Olarak Değerlendirilmesi

Son yıllarda yapılan çalışmalar, birçok hastalığın oluşumunda ve yaşlanmada etkili olan serbest radikallere karşı koruyucu ve geciktirici etkiye sahip antioksidanlar üzerinde yoğunlaşmaktadır. Bitkisel ve hayvansal dokularda bulunan doğal antioksidanlar, gıdalarla birlikte vücuda alınarak savunma sistemini güçlendirmektedir. Bu doğal bileşenlerin, toksisite nedeniyle uygulamaları giderek kısıtlanan sentetik antioksidanlar yerine katkı maddesi olarak gıda ve diğer endüstriyel sektörlerde uygulamaları üzerinde çalışmalar artmaktadır. Tarımsal hammaddelerin endüstriyel olarak işlenmesi sırasında önemli miktarda atık ve yan ürünler oluşmaktadır. Bunlar arasında nar yan ürünleri (nar çekirdeği, kabuğu, çiçeği vs) yüksek fenolik içeriğine bağlı olarak yüksek biyoaktif bileşen miktarları ile ön plana çıkmaktadır. Bu makalede öncelikle antioksidan bileşikler ile mekanizmaları özetlenmiş ve nar yan ürünlerinin antioksidatif ve antikarsinojen gibi terapötik etkileri incelenmiştir.

Natural Antioxidant Compounds: Potential Use of Pomegranate By-Products as Antioxidants

In recent years, the focus has been on antioxidants that have preventive and retarding effects on free radicals that play important roles in aging process and result in many diseases. Natural antioxidants, present in plant and animal tissues, are taken with daily diet to enhance the immune function. Obtaining these compounds are becoming important instead of restricted synthetical antioxidants. Therefore, the interest has been increased in detection and applicability of novel antioxidants obtained from natural sources in foods or other industries as additive instead of synthetic antioxidants, which are being restricted due to their toxicity. During industrial food processes significant amount of renewable or nonrenewable wastes and by-products are generated, which represent a major disposal problem aside with loss of bioactive compounds. Among these wastes, pomegranate by-products (seeds, peels, flowers, etc) could offer practical and economical sources with therapeutic potential, due to high content of bioactive compounds. This article summarizes antioxidative compounds and their mechanisms, aside with therapeutic properties of pomegranate byproducts in terms of antioxidative and anticarcinogenic effects.

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Adams, L. S., Y. Zhang, N.P. Seeram, D. Heber, and S. Chen. 2010. Pomegranate ellagitanninderived compounds exhibit antiproliferative and antiaromatase activity in breast cancer cells in vitro. Cancer Prevention Research, 3 (1):108-113.
Akbarpour, V., K. Hemmati, and M. Sharifani. 2009. Physical and chemical properties of pomegranate (Punica granatum L.) fruit in maturation stage. The American-Eurasian Journal Agriculture and Environmental Science, 6 (4): 411-416.
Aktaş, B., P. Özdemir ve H. Basmacıoğlu-Malayoğlu. 2013. Bazı agro-endüstriyel yan ürünlerin doğal antioksidan kaynağı olarak değerlendirilmesi. Hayvansal Üretim, 54 (2): 30-35.
Anonim, 2014. TÜİK, Bitkisel Üretim İstatistikleri (1988-2014). http://www.tuik.gov.tr/PreTablo.do?alt_id=1001 (Erişim Tarihi: 05.08.2015).
Ashton, R. 2006. Meet the Pomegranate. pp: 3-8 Editörler: R. Ashton, B. Baer, D. Silverstein. Incredible Pomegranate: Plant and Fruit. Third Millenium Publications, AZ, USA.
Bekir, J., M. Mars, P. Vicendo, A. Fterrich and J. Bouajila. 2013. Chemical composition and antioxidant, anti-inflammatory, and antiproliferation activities of pomegranate (Punica granatum) flowers. Journal of Medicinal Food, 16 (6): 544-550.
Cheng, S.H., J.Y. Zhuang, Y.Y. Fan, J.H. Du and L.Y. Cao. 2007. Progress in research and development in hybrid rice: a super-domesticate in China. Annals of Botany, 100 (5): 959-966.
Çolak, H. ve B.H. Ulusoy. 2005. Bitkisel orijinli gıdalarda bulunan bazı doğal antioksidan maddeler ve etkileri. Gıda ve Yem Bilimi Teknolojisi, 8 (4): 43-48.
de Lourdes Reis Giarda, M. 2013. Food Phenolic Compounds: Main Classes, Sources and Their Antioxidant Power. pp: 87-112. Editör: J.A. Morales-Gonzalez. Oxidative Stress and Chronic Degenerative Diseases - A Role for Antioxidants. Intech Open Books.
Decker, E.A., B. Chen, A. Panya, and R.J. Elias. 2010. Understanding antioxidant mechanisms in preventing oxidation in foods. pp: 225-248. Editörler: E.A. Decker, R.J Ellias, D.J McClements. Volume 1: Understanding Mechanisms of Oxidation and Antioxidant Activity. Oxidation in Foods and Beverages and Antioxidant Applications. Woodhead Publishing, Cambridge.
Dikmen, M., N. Ozturk and Y. Ozturk. 2011. The antioxidant potency of Punica granatum L. fruit peel reduces cell proliferation and induces apoptosis on breast cancer. Journal of Medicinal Food, 14 (12): 1638-1646.
Dimitrios, B. 2006. Sources of natural phenolic antioxidants. Trends in Food Science & Technology, 17 (9): 505-512.
Duda-Chodak, A. and T. Tarko. 2007. Antioxidant properties of different fruit seeds and peels. Acta Scientiarum Polonorum Technologia Alimentaria, 6 (3): 29-36.
Ekşi, A. ve İ. Özhamamcı. 2009. Chemical composition and guide values of pomegranate juice. Gıda, 34 (5): 265-270.
El-Baroty, G.S., M.F. Khalil and S.H.A. Mostafa. 2014. Natural antioxidant ingredient from byproducts of fruits. American Journal of Agricultural and Biological Sciences, 9 (3): 311-320.
El-Nemr, S.E., I.A. Ismail and M. Ragab. 1990. Chemical composition of juice and seeds of pomegranate fruit. Food / Nahrung, 34 (7): 601-606.
El-Said, M.M., H.F. Haggag, H.M. Fakhr El-Din, A.S. Gad and A.M. Farahat. 2014. Antioxidant activities and physical properties of stirred yoghurt fortified with pomegranate peel extracts. Annals of Agricultural Science, 59 (2): 207-212.
Faria, A. and C. Calhau. 2011. The bioactive of pomegranate: impact on health and disease. Critical Reviews in Food Science and Nutrition, 51 (7): 626-634.
Fernandez-Panchon, M.S., D. Villano, A.M. Troncoso and M.C. Garcia-Parrilla. 2008. Antioxidant activity of phenolic compounds: from in vitro results to in vivo evidence. Critical Reviews in Food Science and Nutrition, 48 (7): 649-671.
Fischer, U.A., R. Carle and D.R. Kammerer. 2011. Identification and quantification of phenolic compounds from pomegranate (Punica granatum L.) peel, mesocarp, aril flavonols and flavones changes in pomegranate and differently produced juices by HPLC DAD-ESI/MSn. Food Chemistry, 127: 807-821.
Gil, M.I., F.A. Tomas-Barberan, B. Hess-Pierce, D.M. Holcroft and A.A. Kader. 2000. Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. Journal of Agricultural and Food Chemistry, 48 (10): 4581-4589.
Guo, C., J. Yang, J. Wei, Y. Li, J. Xu and Y. Jiang. 2003. Antioxidant activities of peel, pulp and seed fractions of common fruits as determined by FRAP assay. Nutrition Research, 23 (12): 1719- 1726.
Gutiérrez-Larraínzar, M., J. Rúa, I. Caro, C. de Castro, D. de Arriaga, M.R. García-Armesto and P. del Valle. 2012. Evaluation of antimicrobial and antioxidant activities of natural phenolic compounds against foodborne pathogens and spoilage bacteria. Food Control, 26 (2): 555-563.
Heber, D. 2011. Pomegranate Ellagitannins. Editörler: I.F.F. Benzie and S. Wachtel-Galor. Herbal Medicine: Biomolecular and Clinical Aspects. Boca Raton (FL): CRC Press. http://www.ncbi.nlm.nih.gov/pubmed/22593938.
Hybertson, B.M., B. Gao, S.K. Bose and J.M. McCord. 2011. Oxidative stress in health and disease: the therapeutic potential of Nrf2 activation. Molecular Aspects of Medicine, 32 (4-6): 234-246.
Iqbal, S., S. Haleem, M. Akhtar, M. Zia-ul-Haq and J. Akbar. 2008. Efficiency of pomegranate peel extracts in stabilization of sunflower oil under accelerated conditions. Food Research International, 41: 194-200.
Jing, P., T. Ye, H. Shi, Y. Sheng, M. Slavin, B. Gao, L. Liu and L.L. Yu. 2012. Antioxidant properties and phytochemical composition of China-grown pomegranate seeds. Food Chemistry, 132 (3): 1457-1464.
Karpinska, M., J. Borowski and M. Danowska-Oziewicz. 2001. The use of naturel antioxidants in ready-to-serve foods. Food Chemistry, 72 (1): 5-9.
Kaur, C. and H.C. Kapoor. 2001. Antioxidants in fruits and vegetables - The millennium's health. International Journal of Food Science and Technology, 36 (7): 703-725.
Kaur, G., Z. Jabbar, M. Athar and M.S. Alam. 2006. Punica granatum (pomegranate) flower extract possesses potent antioxidant activity and abrogates Fe-NTA induced hepatotoxicity in mice. Food and Chemical Toxicology, 44 (7): 984-993.
Khansari, N., Y. Shakiba and M. Mahmoudi. 2009. Chronic inflammation and oxidative stress as a major cause of age-related diseases and cancer. Recent Patents on Inflammation & Allergy Drug Discovery, 3 (1): 73-80.
Koolen, H.H.F., F.M.A. da Silva, F.C. Gozzo, A.Q.L. de Souza and A.D.L. de Souza. 2013. Antioxidant, antimicrobial activities and characterization of phenolic compounds from Buriti (Mauritia flexuosa L. f.) by UPLC-ESI-MS/MS. Food Research International, 51 (2): 467-473.
Kraovicová, J. and P. Simko. 2000. Determination of synthetic phenolic antioxidants in food by highperformance liquid chromatography. Journal of Chromotography, 882 (1-2): 271-281.
Kris-Etherton, P.M., K.D. Hecker, A. Bonanome, S.M. Coval, A.E. Binkoski, K.F. Hilpert, A.E. Griel and T.D. Etherton. 2002. Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer. American Journal of Medicine, 113 (Suppl 9B): 71-88.
Kulkarni, A.P. and S.M. Aradhya. 2005. Chemical changes and antioxidant activity pomegranate arils during fruit development. Food Chemistry, 93 (2): 319-324.
Kumar, S., R. Singh, R. Asrey and D.D. Nangare. 2012. Techno-economic evaluation of integrated canal water harvesting and drip irrigation for pomegranate production in a dry eco-system. Irrigation and Drainag, 61: 366-374.
Kurt, H. ve G. Şahin. 2013. Bir ziraat coğrafyası çalışması: Türkiye'de nar (Punica granatum L.) tarımı. Marmara Coğrafya Dergisi, 27 (1): 551-574.
Li, Y., C. Guo, J. Yang, J. Wei, J. Xu and S. Cheng. 2006. Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chemistry, 96 (2): 254-260.
Liochev, S.I..2013. Reactive oxygen species and the free radical theory of aging. Free Radical Biology and Medicine, 60: 1-4.
Lushchak, V.I. 2014. Free radicals, reactive oxygen species, oxidative stress and its classification. Chemico-Biological Interactions, 224: 164-175.
Madrigal-Carballo, S., G. Rodriguez, C. Krueger, M. Dreher and J. Reed. 2009. Pomegranate (Punica granatum) supplements: authenticity, antioxidant and polyphenols composition. Journal of Functional Foods, 1: 324-329.
Mansour, E., A.B. Khaled, B. Lachiheb, M. Abid, Kh. Bachar and A. Ferchichi. 2013. Phenolic compounds, antioxidant, and antibacterial activities of peel extract from Tunisian pomegranate. Journal of Agricultural Science and Technology, 15 (Suppl): 1393-1403.
Meral, R., İ.S. Doğan ve G.S. Kanberoğlu. 2012. Fonksiyonel gıda bileşeni olarak antioksidanlar. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 2 (2): 45-50.
Mizrahi, M., Y. Friedman-Levi, L. Larush, K. Frid, O. Binyamin, D. Dori, N. Fainstein, H. Ovadia, T.Ben-Hur, S. Magdassi and R. Gabizon. 2014. Pomegranate seed oil nanoemulsions for the prevention and treatment of neurodegenerative diseases: the case of genetic CJD. Nanomedicine, 10 (6): 1353-1363.
Mohammad, S.M. and H.H. Kashani. 2012. Chemical composition of the plant Punica granatum L. and its effect on heart and cancer. Journal of Medicinal Plants Research, 6 (40): 5306-5310.
Movileanu, I., M.T. Núñez de González, B. Hafley, R.K. Miller and J.T. Keeton. 2013. Comparison of dried plum puree, rosemary extract, and BHA/BHT as antioxidants in irradiated ground beef patties. International Journal of Food Science, Article ID 360732.
Negi, P. and J. Jayaprakasha. 2003. Antioxidant and antibacterial activities of Punica granatum peel extracts. Journal of Food Science, 68: 1473-1477.
Nichenametla, S.N., T.G. Taruscio, D.L. Barney and J.H. Exon. 2006. A review of the effects and mechanisms of polyphenolics in cancer. Critical Reviews in Food Science and Nutrition, 46 (2): 161-183.
Orak, H.H., H. Yağar ve S.S. İşbilir. 2012. Comparison of antioxidant activities of juice, peel and seed of pomegranate (Punica granatum L.) and inter-relationships with total phenolic, tannin, anthocyanin, and flavonoid contents. Food Science Biotechnology, 21: 373-387.
Orgil, O., E. Schwartz, L. Baruch, I. Matityahu, J. Mahajna and R. Amir. 2014. The antioxidative and anti-proliferative potential of non-edible organs of the pomegranate fruit and tree. Food Science and Technology, 58 (2): 571-577.
Pellegrini, N., M. Serafini, S. Salvatore, D.D. Rio, M. Bianchi and F. Brighenti. 2006. Total antioxidant capacity of spices, dried fruits, nuts, pulses, cereals and sweets consumed in Italy assessed by three different in vitro assays. Molecular Nutrition and Food Research, 50 (11): 1030-1038.
Prakash, C.V.S. and I. Prakash. 2011. Bioactive chemical constituents from pomegranate (Punica granatum) juice, seed and peel - a review. International Journal of Research in Chemistry and Environment, 1 (1): 1-18.
Reuter S., S.C. Gupta, M.M. Chaturvedi and B.B. Aggarwal. 2010. Oxidative stress, inflammation, and cancer: how are they linked. Free Radical Biology & Medicine, 49 (11): 1603-1616.
Singh, R.P., K.N. Chidambara-Murthy and G.K. Jayaprakasha. 2002. Studies on the antioxidant activity of pomegranate (Punica granatum) peel and seed extracts using in vitro models. Journal of Agricultural and Food Chemistry, 50 (1): 81-86.
Shiban, M.S., M.M. Al Otaibi, N.S. Al-Zoreky. 2012. Antioxidant activity of pomegranate (Punica granatum L.) fruit peels. Food Nutritional Science, 3: 991-996.
Syed, D.N., F. Afaq and H. Mukhtar. 2007. Pomegranate derived products for cancer chemoprevention. Seminars in Cancer Biology, 17 (5): 377-385.
Tehranifar, A., Y. Selahvarzi, M. Kharrazi and V.J. Bakhsh. 2011. High potential of agro-industrial by-products of pomegranate (Punica granatum L.) as the powerful antifungal and antioxidant substances. Industrial Crops and Products, 34 (3): 1523-1527.
Tekgül, Y. ve F. Kök. 2011. Narın sağlık üzerine etkileri. Hasad Gıda, 318: 26-29.
Tsao, R. and Z. Deng. 2004. Separation procedures for naturally occurring antioxidant phytochemicals. Journal of Chromatography B, 812 (1-2): 85-99.
Vermerris W. and R. Nicholson. Families of Phenolic Compounds and Means of Classification. 2006. pp: 1-34. Editörler: W. Vermerris and R. Nicholson. Phenolic Compound Biochemistry. Springer, Netherlands.
Viuda-Martos, M., J. Fernández-López and J.A. Pérez-Álvarez. 2010. Pomegranate and its many functional components as related to human health: a review. Comprehensive Reviews in Food Science and Food Safety, 9 (6): 635-654.
Yılmaz, B. ve C. Usta. 2010. Narın (Punica granatum) terapötik etkileri. Türk Aile Hekimliği Dergisi, 14 (3): 146-153.
Wang, R., Y. Ding, R. Liu, L. Xiang and L. Du. 2010. Pomegranate: constituents, bioactivities and pharmocokinetics. Fruit, Vegetable and Cereal Science and Biotechnology, 2: 77-87.
Zhang, L., X. Yang, Y. Zhang, L.Wang and R. Zhang. 2011. In vitro antioxidant properties of different parts of pomegranate flowers. Food and Bioproducts Processing, 89 (3): 234-240.
Zhao, X., Z. Yuan, Y. Fang, Y. Yin, and L. Feng. 2013. Characterization and evaluation of major anthocyanins in pomegranate (Punica granatum L.) peel of different cultivars and their development phases. Europe Food Research and Technology, 236: 109-117.