Sema SERTER KOÇOĞLU, Levent ELMAS, Mücahit SEÇME

Erianinin HT29 Kolorektal Kanser Hücrelerinin Proliferasyonu ve Koloni Oluşumu Üzerine Etkilerinin Araştırılması

Kolorektal kanser, dünyada kanser ilişkili ölümlerin en yaygın dördüncü sebebidir. Erianin antioksidan ve anti-tümör etkilere sahip Dendrobium ekstraktından elde edilen yeni bir dibenzil bileşiğidir. Bu çalışmada, erianinin HT29 kolorektal kanser hücreleri üzerine olan terapötik etkileri araştırılmıştır. Erianinin HT29 hücre canlılığı üzerine etkileri XTT test ile koloni oluşumu üzerine etkileri ise koloni formasyonu ile değerlendirilmiştir. Erianinin HT29 hücrelerinde IC50 değeri 48. saatte 59.05 μM olarak belirlenmiştir. HT29 hücre dizisinde erianin uygulanan grupta koloni sayısı 67±33 iken kontrol grubunda 350±89 olarak hesaplanmıştır. Erianin, HT29 kolorektal kanser hücrelerinde koloni oluşumunu ise anlamlı derecede azaltmıştır. Yapılan çalışmaların sonuçları, erianinin kolorektal kanser tedavisinde doğal elde edilen bir bileşik olarak güvenli, kolay ulaşılabilir ve umut veren terapötik bir ilaç olabileceğini destekler niteliktedir. Gelecekte erianinin kolorektal kanser hücreleri üzerindeki etki mekanizmasını aydınlatacak daha kapsamlı ve çok merkezli desteklenecek ileri düzeyde klinik çalışmalara ihtiyaç vardır.

Anahtar Kelimeler:

erianin, HT29, kolorektal kanser

Investigation of the effects of erianin on proliferation and colony formation of HT29 colorectal cancer cells

Colorectal cancer (CRC) is the 4th most common cause of cancer-related death in the global world. Erianin, a novel dibenzyl compound in Dendrobium extract, has antioxidative and antitumor activities. In this study, the therapeutic effects of erianin on HT29 colorectal cancer cells were investigated. Effects of erianin on cell viability were evaluated by XTT test. Effects of erianin on colony formation were evaluated by colony formation analysis. IC50 values of Erianin on HT29 cells were determined as 59.05 μM at 48th hour. While the number of colonies in the HT29 cell line was 67±33 in the erianin treated group, it was calculated as 350±89 in the control group. Erianin significantly reduced colony formation in HT29 colorectal cancer cells. The results of the presented studies support that erianin as a natural product in the treatment of colorectal cancer can be a safe, easily accessible and promising therapeutic drug. In the future, more comprehensive and multi-center supported clinical studies are needed to elucidate the mechanism of action of erianin on colorectal cancer cells.

Keywords:

erianin, HT29, colorectal cancer,

PDF

___

An BC, Hong S, Park HJ, vd. Anti-Colorectal Cancer Effects of Probiotic-Derived p8 Protein. Genes 2019; 10(8). doi:10.3390/genes10080624
Weng W, Goel A. Curcumin and colorectal cancer: An update and current perspective on this natural medicine. Seminars in Cancer Biology 2020. doi:10.1016/j.semcancer.2020.02.011
Huang X mei, Yang Z jie, Xie Q, Zhang Z kang, Zhang H, Ma J ying. Natural products for treating colorectal cancer: A mechanistic review. Biomedicine and Pharmacotherapy 2019; 117: 109142. doi:10.1016/j.biopha.2019.109142
Chung SS, Dutta P, Chard N, vd. A novel curcumin analog inhibits canonical and non-canonical functions of telomerase through STAT3 and NF-κB inactivation in colorectal cancer cells. Oncotarget 2019; 10(44): 4516–4531. doi:10.18632/oncotarget.27000
Gamage CDB, Park SY, Yang Y, vd. Deoxypodophyllotoxin exerts anti-cancer effects on colorectal cancer cells through induction of apoptosis and suppression of tumorigenesis. International Journal of Molecular Sciences 2019; 20(11): 2612. doi:10.3390/ijms20112612
Wang S, Wang L, Zhou Z, vd. Leucovorin Enhances the Anti-cancer Effect of Bortezomib in Colorectal Cancer Cells. Scientific Reports 2017; 7(1): 682 doi:10.1038/s41598-017-00839-9
Wang X, Li T. Ropivacaine inhibits the proliferation and migration of colorectal cancer cells through ITGB1. Bioengineered 2021; 12(1): 44–53. doi:10.1080/21655979.2020.1857120
Hlosrichok A, Sumkhemthong S, Sritularak B, Chanvorachote P, Chaotham C. A bibenzyl from Dendrobium ellipsophyllum induces apoptosis in human lung cancer cells. Journal of Natural Medicines 2018; 72(3): 615–625. doi:10.1007/s11418-018-1186-x
Guo Z, Zhou Y, Yang J, Shao X. Dendrobium candidum extract inhibits proliferation and induces apoptosis of liver cancer cells by inactivating Wnt/β-catenin signaling pathway. Biomedicine and Pharmacotherapy 2019; 110: 371–379. doi:10.1016/j.biopha.2018.11.149
Yang L, Hu Y, Zhou G, Chen Q, Song Z. Erianin suppresses hepatocellular carcinoma cells through down-regulation of PI3K/AKT, p38 and ERK MAPK signaling pathways. Bioscience reports 2020; 40(7): BSR20193137. doi:10.1042/BSR20193137
Li M, He Y, Peng C, Xie X, Hu G. Erianin inhibits human cervical cancer cell through regulation of tumor protein p53 via the extracellular signal-regulated kinase signaling pathway. Oncology Letters 2018; 16(4): 5006–5012. doi:10.3892/ol.2018.9267
Zhu Q, Sheng Y, Li W, vd. Erianin, a novel dibenzyl compound in Dendrobium extract, inhibits bladder cancer cell growth via the mitochondrial apoptosis and JNK pathways. Toxicology and Applied Pharmacology 2019; 371: 41–54. doi:10.1016/j.taap.2019.03.027
Wang H, Zhang T, Sun W, vd. Erianin induces G2/M-phase arrest, apoptosis, and autophagy via the ROS/JNK signaling pathway in human osteosarcoma cells in vitro and in vivo. Cell Death and Disease 2016; 7(6): e2247. doi:10.1038/cddis.2016.138
Chen P, Wu Q, Feng J, vd. Erianin, a novel dibenzyl compound in Dendrobium extract, inhibits lung cancer cell growth and migration via calcium/calmodulin-dependent ferroptosis. Signal Transduction and Targeted Therapy. 2020; 5(1): 51. doi:10.1038/s41392-020-0149-3
Sun J, Fu X, Wang Y, vd. Erianin inhibits the proliferation of T47D cells by inhibiting cell cycles, inducing apoptosis and suppressing migration. American journal of translational research. 2016;8(7):3077–3086.
Rajendran V, Jain MV. In vitro tumorigenic assay: colony forming assay for cancer stem cells. Içinde: Methods in Molecular Biology 2018; 1692: 89–95. doi:10.1007/978-1-4939-7401-6_8
Franken NAP, Rodermond HM, Stap J, Haveman J, van Bree C. Clonogenic assay of cells in vitro. Nature Protocols 2006; 1(5): 2315–2319. doi:10.1038/nprot.2006.339
Brenner H, Kloor M, Pox CP. Colorectal cancer. The Lancet 2014; 383: 1490–1502. doi:10.1016/S0140-6736(13)61649-9
Ruiz de Porras V, Layos L, Martínez-Balibrea E. Curcumin: A therapeutic strategy for colorectal cancer? Seminars in Cancer Biology 2021; 73: 321–330. doi:10.1016/j.semcancer.2020.09.004
Khan I, Mahfooz S, Ansari IA. Antiproliferative and Apoptotic Properties of Andrographolide Against Human Colon Cancer DLD1 Cell Line. Endocrine, Metabolic & Immune Disorders - Drug Targets 2019; 20(6): 930–942. doi:10.2174/1871530319666191125111920
Liu YT, Hsieh MJ, Lin JT, vd. Erianin induces cell apoptosis through ERK pathway in human nasopharyngeal carcinoma. Biomedicine and Pharmacotherapy 2019; 111: 262–269. doi:10.1016/j.biopha.2018.12.081
Zhu Q, Sheng Y, Li W, vd. Erianin, a novel dibenzyl compound in Dendrobium extract, inhibits bladder cancer cell growth via the mitochondrial apoptosis and JNK pathways. Toxicology and Applied Pharmacology 2019; 371: 41–54. doi:10.1016/j.taap.2019.03.027
Cho JG, Lim KH, Park SG. MED28 increases the colony-forming ability of breast cancer cells by stabilizing the ZNF224 protein upon DNA damage. Oncology Letters 2018; 15(3): 3147–3154. doi:10.3892/ol.2017.7718