KADMİYUM, KURŞUN VE ÇİNKO METALLERİNİN MARUL (Lactuca sativa L.) TOHUMLARININ ÇİMLENME ÖZELLİKLERİ ÜZERİNE ETKİSİ

Bitkiler, toprak ve yeraltı suyu/atmosfer arasındaki en büyük arabirim olarak görev yapmakta olup, yaşamları boyunca birçok stres faktörü ile karşı karşıya kalırlar. Bitki gelişimi için gerekli olan mikro besin elementleri (bakır (Cu), çinko (Zn), demir (Fe) vb.) ile toprakta bulunan ağır metaller (kobalt (Co), kurĢun (Pb), kadmiyum (Cd) vb.) belirli konsantrasyonların üzerinde ve çözünürlüklerine bağlı olarak fitotoksik etkilere sahiptirler. Bu metaller birçok farklı yolla ekosisteme dahil olurlar. Endüstriyel kaynaklı metallerin baĢında Cu, nikel (Ni), Pb, Zn ve Cd gelmektedir. En önemli sorun bu metallerin besin zincirine girme olasılığıdır. Bu çalıĢmada farklı konsantrasyonlardaki Zn (5, 10, 20, 40, 80, 160 mg L -1 ), Cd (0, 1, 2, 4, 8, 16, 32 mg L-1 ) ve Pb (0, 2, 4, 8, 16, 32, 64, 128, 256 mg L-1 ) metallerinin marul (Lactuca sativa L.) bitkisinin tohum çimlenmesi, kök ve gövde uzaması ve bitki biyokütlesi üzerine etkileri araĢtırılmıştır. Sonuçta Zn ve Cd uygulamaları tohum çimlenmesini azaltmıĢtır. Pb uygulaması ise düĢük konsantrasyonlarda çimlenmeyi arttırırken yüksek konsantrasyonlarda tohum çimlenmesinde inhibisyona sebep olmuştur. Her üç metal maruziyetinde kök ve gövde uzamasında inhibisyonun olduğu ve kök inhibisyonunun gövdeye göre daha duyarlı olduğu belirlenmiştir. Bitki kuru kütlelerinde kontrole kıyasla büyük bir değişiklik gözlenmezken, yaş biyokütlelerde özellikle Zn maruziyetinde önemli azalmalar saptanmıştır.

Effects of Cadmium, Lead and Zinc on Seed Germination Characteristics of Lettuce (Lactuca sativa L.)

Plants are the largest interface between soil and groundwater/atmosphere. Plants face many stress factors throughout their lives. The micronutrients (copper (Cu), zinc (Zn), iron (Fe), etc.) required for plant growth and some toxic metals (cobalt (Co), lead (Pb), cadmium (Cd) etc.) found in soil have phytotoxic effects depending on their specific solubility and concentrations. These heavy metals are involved in the ecosystem in many different ways. At the beginning of industrial welded metals are Cu, nickel (Ni), Pb, Zn and Cd. The most important problem is the entering possibility of these heavy metals to the food chain. In this study, different doses of Zn (5, 10, 20, 40, 80, 160 mg L -1 ), Cd (0, 1, 2, 4, 8, 16, 32 mg L -1 ) and Pb (0, 8, 16, 32, 64, 128, 256 mg L -1 ) effects on the seed germination, root and shoot elongation and plant biomass of lettuce were studied. As a result, Zn and Cd treatments reduced seed germination. Pb treatment was increased the seed germination at low concentrations, whereas it was inhibited the seed germination at high concentrations. Inhibition of root and shoot elongation was observed at concentration of all metals and it was found that roots are more sensitive than shoot. While there was no significant change in plant dry masses compared to control, significant decreases were detected in wet biomass, especially Zn exposure.

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  • Acero P, MandadoJ M.A., Gomez J., Gimeno M., Auque L. ve Torrijo F. (2003) Environmental impact of heavy metal dispersion in the Huerva River (Iberian range, NE Spain), Environmental Geology, 43, 950–956.
  • Afrakhteh S., Frahmandfar E., Hamidi A. ve Ramandi H.D. (2013) Evaluation of growth characteristics and seedling vigor in two cultivars of soybean dried under different temperature and fluidized bed dryer, International Journal of Agriculture and Crop Sciences, 5(21), 2537-2544.
  • Anonim, 2008, https://www.canada.ca/en/environment-climate-change/services/wildliferesearch-landscape-science/biological-test-method-publications/terrestrial-plantscontaminants-soil.html, Erişim Tarihi: 17.04.2018, Konu: Biological test method for measuring terrestrial plants exposed to contaminants in soil: chapter 1
  • ATSDR (2017) https://www.atsdr.cdc.gov/spl/index.html, Erişim Tarihi: 25.03.2018, Konu: Agency for Toxic Substances and Disease Registry (ATSDR)'s Substance Priority List.
  • Ayaz F.A. ve Kadıoğlu A. (1997) Effects of metals (Zn, Cd, Cu, Hg) on the soluble protein bands of germinating Lens esculenta L. Seeds, Turkish Journal of Botany, 21, 85–88.
  • Barman S.C., Sahu R.K., Bhargava S.K. ve Chaterjee C. (2000) Distribution of metals in wheat, mustard, and weed grown in field irrigated with industrial effluents, Bulletin of Environmental Contamination and Toxicology, 64, 489–496.
  • Barrameda-Medina Y., Montesinos-Pereira D., Romero L., Ruiz J. M. ve Blasco B. (2014) Comparative study of the toxic effect of Zn in Lactuca sativa and Brassica oleracea plants: I. Growth, distribution, and accumulation of Zn, and metabolism of carboxylates, Environmental and Experimental Botany, 107, 98–104.
  • Claire L.C., Adriano D.C., Sajwan K.S., Abel S.L., Thoma D.P. ve Driver J.T. (1991) Effects of selected trace metals on germinating seeds of six plant species, Water, Air, and Soil Pollution, 59, 231-240.
  • Delgado M., Bigeriego M. ve Guardiola E. (1993) Uptake of Zn, Cr and Cd by water hyacinths, Water Research, 27(2), 269-272.
  • Edelstein M. ve Ben-Hur M. (2018) Heavy metals and metalloids: Sources, risks and strategies to reduce their accumulation in horticultural crops, Scientia Horticulturae, 234, 431-444.
  • Espen L., Pirovano L. ve Cocucci S.M (1997) Effects of Ni2+ during the early phases of radish (Raphanus sativus) seed germination, Environmental and Experimantal Botany, 38, 187–197.
  • Fargasova A. (1994) Effect of Pb, Cd, Hg, As and Cr on germination and root growth of Sinapsis alba seeds, Bulletin of Environmental Contamination and Toxicology, 52, 452–456.
  • Foy C.D., Chaney R. L. ve White M.C. (1978) The physiology of metal toxicity in plants, Annual Review of Plant Physiology., 29, 511-66.
  • Gharebaghi A., Haghighi A.M.H. ve Arouiee H. (2017) Effect of cadmium on seed germination and earlier basil (Ocimum basilicum L. and Ocimum basilicum var. purpurescens) seedling growth, Trakia Journal of Sciences, 1, 1-4.
  • Jibril S.A., Hassan S.A., Ishak, C.F. ve Wahab P.E.M. (2017) Cadmium toxicity affects phytochemicals and nutrient elements composition of lettuce (Lactuca sativa L.), Advances in Agriculture, 1- 7.
  • Judia C. ve Fulekar M.H. (2008) Phytoremediation: the application of vermicompost to remove zinc, cadmium, copper, nickel and lead by sunflower plant, Environmental Engineering and Management Journal, 7(5), 547-558.
  • Khan S., Cao Q., Zheng Y.M., Huang Y.Z. ve Zhu Y.G. (2008) Health risks of heavy metals in contaminated soils and food crops irrigated with wastewater in Beijing, China, Environmental Pollution, 152, 686-692.
  • Khan S. U. ve Moheman A. (2006) Effect of heavy metals (Cadmium and Nickel) on the seed germination, growth and metals uptake by chilli (Capsicum frutescens) and sunflower plants (Helianthus annuus), Pollution Research, 25, 99-104.
  • Kapustka L.A. ve Reporter M. (1993) Terrestrial primary producers. In: Peter Calow (ed) Handbook of ecotoxicology, vol. 1. Blackwell Scientific Publications, 278–298.
  • Karakaş S., Çullu M.A. ve Dikilitaş M. (2015) In vitro koĢullarda halofit bitkilerden salsola soda ve portulaca oleracea’nın nacl stresine karşı çimlenme ve gelişim durumları, Harran Tarım ve Gıda Bilimleri Dergisi, 19(2), 66-74.
  • Lyu J., Park J., Pandey K.L., Choid S., Leed H., Saeger J.D., Depuydt S. ve Han T. (2018) Testing the toxicity of metals, phenol, effluents, and receiving waters by root elongation in Lactuca sativa L., Ecotoxicology and Environmental Safety, 149, 225-232.
  • Lidon F.C. ve Henriques F.S. (1998) Role of rice shoot vacuoles in copper toxicity regulation, Environmental and Experimental Botany, 39, 197-202.
  • Ma W.Z., Ma W.M., Du Y.Y., Dan Q.P., Yin B., Dai S. S. ve Hao X. (2018) Toxicity Effect of Cr Stress on Seed Germination and Seedling Growth in Lactuca Sativa, IOP Conf. Series: Earth and Environmental Science 128, 012131.
  • Mahmoodzadeh H., Nabavi M. ve Kashefi H. (2013) Effect of nanoscale titanium dioxide particles on the germination and growth of canola (Brassica napus). Journal of Ornamental Horticultural Plants, 3, 25–32.
  • Mesmar M.N. ve Jaber K. (1991) The toxic effect of lead on seed germination, growth, chlorophyll and protein contents of wheat and lens, Acta Biologica Hungarica, 42, 331-344.
  • Mishra A., Choudhuri M.A. (1998) Amelioration of lead and mercury effects on germination and rice seedling growth by antioxidants, Biologia Plantarum, 41, 469-473.
  • Moustakas M., Lanaras T., Symeonidis L. ve Karataglis S. (1994) Growth and some photosynthetic characteristics of field grown Avena sativa under copper and lead stress, Photosynthetica, 30, 389-396.
  • Munzuroğlu Ö. ve Geçkil H. (2002) Effects of metals on seed germination, root elongation, and coleoptile and hypocotyl growth in Triticum aestivum and Cucumis sativus, Archives of Environmental Contamination and Toxicology, 43, 203-213.
  • Nedelkoska T.V. ve Doran P.M. (2000) Characteristics of heavy metal uptake by plants species with potential for, phytoremediation and phytomining, Minerals Engineering, 13, 549-561.
  • Okcu M., Tozlu E., Kumlay A.M. ve Pehluvan M. (2009) Ağır Metallerin Bitkiler Üzerine Etkileri, Alınteri, 17(B), 14-26.
  • Öncel I., Keles Y. ve Üstün A.S. (2000) Interactive effects of temperature and heavy metal stress on the growth and some biochemical compounds in wheat seedlings, Environmental Pollution, 107, 315-320.
  • Peralta J.R., Gardea-Torresdey J.L., Tiemann K.J., Gomez E., Arteaga S., Rascon E. ve diğ. (2001) Uptake and effects of five heavy metals on seed germination and plant growth in alfalfa (Medicago sativa L.), Bulletin of Environmental Contamination and Toxicology, 66, 727-734.
  • Phalsson A.M.B. (1989) Toxicity of heavy metals (Zn, Cu, Cd, Pb) to vascular plants, Water, Air, Soil Pollution, 47, 287-319.
  • Priac A., Badot P.M. ve Crini G. (2017) Treated wastewater phytotoxicity assessment using Lactuca sativa: Focus on germination and root elongation test parameters, Comptes Rendus Biologies, 340, 188–194.
  • Poschenrieder, C., Gunse B. ve Barcelo J. (1989) Influence of cadmium on water relations, stomatal resistance and abscisic acid content in expanding bean leaves, Environmental and Stress Physiology, 90, 1365-1371.
  • Prasad T.N.V.K.V., Sudhakar P., Sreenivasulu Y., Latha P., Munaswamy V., Reddy K.R., Sreeprasad T.S., Sajanlal P.R. ve Pradeep T. (2012) Effect of nanoscale zinc oxide particles on the germination, growth and yield of peanut, Journal of Plant Nutrition, 35(6), 905-927.
  • Ratsch H.C. ve Johndro D. (1984) Comparative toxicity of six test chemicals to lettuce using two root elongation test methods, Environmental Monitoring and Assessment, 6, 267- 276.
  • Ross S.M. (1994) Toxic Metals in Soil–Plant Systems, John Wiley and Sons, Chis Chester.
  • Saether O.M., Krog R., Segar D. ve Storroe G. (1997) Contamination of soil and ground water at former industrial site in Trondheim, Norway, Applied Geochemistry, 12, 327-332.
  • Sacristán D., Recatalá L. ve Viscarra Rossel R.A. (2015) Toxicity and bioaccumulation of Cu in an accumulator crop (Lactuca sativa L.) in different Australian agricultural soils, Scientia Horticulturae 193, 346–352.
  • Sresty T.V.S. ve Madhova K.V. (1999) Ultrastructural alterations in response to zinc and nickel stress in the root cell of pigeonpea, Environmental and Experimental Botany, 41, 3- 13.
  • Silva S., Silva P., Oliveira H., Gaivao I., Matos M., Pinto-Carnide O. ve Santos C. (2017) Pb low doses induced genotoxicity in Lactuca sativa plants, Plant Physiology and Biochemistry 112, 109-116.
  • Silveira G.L., Lima M.G.F., Barreto dos Reis G., Palmieri M.J. ve Andrade-Vieria L.F. (2017) Toxic effects of environmental pollutants: Comparative investigation using Allium cepa L. and Lactuca sativa L., Chemosphere, 178, 359-367.
  • Tiquia S.M., Tam N.F.Y. ve Hodgkiss I.J. (1996) Effects of composting on phytotoxicity of spent pig-manure sawdust litter, Environmental Pollution, 93, 249-256.
  • TKKY, (2005) http://www.resmigazete.gov.tr/eskiler/2005/05/20050531-6.htm, EriĢim Tarihi: 17.04.2018, Konu: Toprak Kirliliği kontrol Yönetmeliği.
  • Vojtechova M. ve Leblova S. (1991) Uptake of lead and cadmium by maize seedlings and the effects of heavy metals on the activity of phosphoenolpyruvate carboxilase isolated from maize. Biologia Plantarum, 33, 386-394.
  • Wolf M., Baretta D., Becegato V.A., Almeida V.C. ve Paulino A.T. (2017) Copper/Zinc Bioaccumulation and the Effect of Phytotoxicity on the Growth of Lettuce (Lactuca sativa L.) in Non-contaminated, Metal-Contaminated and Swine Manure-Enriched Soils, Water Air Soil Pollution, 228, 152, 1-9.
  • Xiong Z. T. (1998) Lead uptake and effects on seed germination and plant growth in a Pb hyperaccumulator Brassica pekinensis Rupr. Bulletin of Environmental Contamination and Toxicology, 60, 285-291.