Netty Maria NAIBAHO, Widya FATRIASARI, Irawan Wijaya KUSUMA, Enos Tangke ARUNG

PHYTOCHEMICAL SCREENING, ANTIOXIDANT AND ANTI-INFLAMMATORY PROPERTIES OF SEVERAL STINGLESS BEE POLLENS PROCESSED USING DIFFERENT DRYING METHODS

Antioxidants play a crucial function in fighting free radicals that can harm biomolecules in the human body and damage cells, which can lead to immunological disorders including inflammation. In this research, we evaluated bee pollen extracts from six stingless bees (Heterotrigona itama, Tetragonula reepeni, Tetragonula testaceitarsis, Tetragonula fuscobalteata, Tetragonula iridipennis, and Tetragonula pagdeni) processed using different drying methods for antioxidant and anti-inflammatory activity. Each sample was divided into two specimens, where one was processed by ovendrying at 40ºC and the second was chilled at 4 ºC, then extracted by ethanol. The extracts of T. fuscobalteata had the highest antioxidant activity when dried in an oven with an IC50 value of 36.47 µg/mL, while T. reepeni using a chiller was 41.30 mg/mL. The highest anti-inflammatory activity was for oven-dried T. fuscobalteata with an IC50 of 39.70 mg/mL, while chilled T. reepeni was 34.30 µg/mL. Different drying techniques can affect the antioxidant and anti-inflammatory activity of bee pollen extracts as well as their potential as food, pharmaceutical and cosmetic ingredients.

Anahtar Kelimeler:

Antioxidant, Anti-inflammatory, Bee pollen, Drying method, Stingless bees

PHYTOCHEMICAL SCREENING, ANTIOXIDANT AND ANTI-INFLAMMATORY PROPERTIES OF SEVERAL STINGLESS BEE POLLENS PROCESSED USING DIFFERENT DRYING METHODS

Keywords:

Antioxidant, Anti-inflammatory, Bee pollen, Drying method, Stingless bees,

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Almeida-Muradian LB, Pamplona LC, Coimbra S, Barth OM. Chemical composition and botanical evaluation of dried bee pollen pellets. Journal of Food Composition and Analysis, 2005;18(1):105–111. doi:https://doi.org/10.1016/j.jfca.2003.10.008
Anjos, Ofélia VP, Delgado T, Estevinho L. Influence of the storage conditions on the quality of bee pollen. Zemdirbyste-Agriculture, 2019;106(1):87–94, doi:10.13080/z-a.2019.106.012
AOAC. Official Methods of Analysis of the Association of Official Analytical Chemists. Marlyand: the Association of Official Analytical Chemists, 2006.
Arung ET, Shimizu K, Kondo R. Inhibitory effect of isoprenoid-substituted flavonoids isolated from Artocarpus heterophyllus on melanin biosynthesis. Planta Med, 2008;72(9):847–850. https://doi.org/10.1055/s-2006-931606
Arung ET, Ramadhan R, Khairunnisa B, Amen Y, Matsumoto M, Nagata M, Wijaya IK, et al. Cytotoxicity effect of honey, bee pollen, and propolis from seven stingless bees in some cancer cell lines. Saudi Journal of Biological Sciences, 2021;28:7182-7189. https://doi.org/10.1016/j.sjbs.2021.08.017
Bai R, Yao C, Zhong Z, Ge J, Bai Z, Ye X, Xie T, Xie Y. Discovery of natural anti-inflammatory alkaloids: Potential leads for the drug discovery for the treatment of inflammation. European Journal of Medicinal Chemistry, 2021;213:18–22 https://doi.org/10.1016/j.ejmech.2021.113165
Bansal Y, Sethi P, Bansal G. Coumarin: a potential nucleus for anti-inflammatory molecules. Med Chem Res, 2012;1-12. doi:DOI 10.1007/s00044-012-0321-6
Barajas J, Cortes-Rodriguez M, Sandova R. Effect of temperature on the drying process of bee pollen from two zones of Colombia. Journal of Food Process Engineering, 2012;35:134–148
Campos MGR, Bogdanov S, Almeida-Muradian LB, Szczesna T, Mancebo Y, Frigerio C, Ferreira F. Pollen composition and standardisation of analytical methods. Journal of Apicultural Research, 2015;47(2):154–161.http://dx.doi.org/10.1080/00218839.2008.11101443
Castagna A, Benelli G, Conte G, Sgherri C, Signorini F, Nicolella C, Ranieri A, Canale A. Drying Techniques and Storage: Do They Affect the Nutritional Value of Bee-Collected Pollen? Molecules, 2020;25:4925. http://dx.doi.org/10.3390/molecules25214925
Cinkmanis I, Dimins F, Mikelsone V. Influence of Lyophilization and convective type drying. Foodbalt, 2017;201-203. doi:10.22616/foodbalt.2017.038
Denisow B, Denisow-Pietrzyk M. Biological and therapeutic properties of bee pollen: a review. Journal Science Food Agric, 2016:1–8. doi:10.1002/jsfa.7729
Dias LG, Tolentino G, Pascoal A, Estevinho LM. Effect of processing conditions on the bioactive compounds and biological properties of bee pollen. Journal of Apicultural Research, 2016;55(5):357–365. http://dx.doi.org/10.1080/00218839.2016.1248109
Diaz P, Chul SJ, Lee S, Khoo C, Rao SK. Antioxidant and anti-inflammatory activities of selected medicinal plants and fungi containing phenolic and flavonoid compounds. Chinese Medicine, 2012;7(26):2–9. http://www.cmjournal.org/content/7/1/26
Fuenmayor C, Zuluaga CD, Díaz MC, Quicazán CM, Cosio M, Mannino S. Evaluation of the physicochemical and functional properties of Colombian bee pollen. Rev MVZ Córdob, 2014;19(1):4003–4014. https://revistamvz.unicordoba.edu.co/article/view/120/189
Harbone JB. Metode Fitokimia (Terjemahan). 2. Bandung: ITB Bandung, 1987
Hassan A, Akmal Z, Khan N. The Phytochemical Screening and Antioxidants Potential of Schoenoplectus triqueter L. Palla. Journal of Chemistry, 2020:1–8. doi:https://doi.org/10.1155/2020/3865139
Isik A, Ozdemir M, Doymaz I. Effect of hot air drying on quality characteristics and physicochemical properties of bee pollen. Food Science and Technology, 2018;1-9. https://doi.org/10.1590/fst.02818
Kahraman HA, Tutun H, Kaya MM, Usluer MS, Tutun S, Yaman C, Sevin S, Keyvan E. Ethanolic extract of Turkish bee pollen and propolis: phenolic composition, antiradical, antiproliferative and antibacterial activities. Biotechnology & Biotechnological Equipment, 2022;36(1):45–56 https://doi.org/10.1080/13102818.2022.2045217
Kalaycıoglu Z, Kaygusuz H, Doker S, Kolaylı S, Erim FB. Characterization of Turkish honeybee pollens by principal component analysis based on their individual organic acids, sugars, minerals, and antioxidant activities. LWT – Food Science and Technology, 2017;84:402–408. http://dx.doi.org/10.1016/j.lwt.2017.06.003
Kocot J, Małgorzata K, Luchowska-Kocot D, Kurzepa J, Musik I. Antioxidant Potential of Propolis, Bee Pollen, and Royal Jelly: Possible Medical Application, Oxidative Medicine and Cellular Longevity, 2018;29:29. https://doi.org/10.1155/2018/7074209
Kokate CK. Pharmacognosy. 16. Mumbai: Niali Prakasham, 2001
Lopes AJO, Vasconcelos CC, Garcia JBS, Pinheiro MSD, Pereira FAN, Camelo DS , Morais SV, et al. Anti‐Inflammatory and Antioxidant Activity of Pollen Extract Collected by Scaptotrigona affinis postica: in silico, in vitro, and in vivo Studies. Antioxidant MDPI, 2020;9 (103):1–16. doi:10.3390/antiox9020103
Martemucci G, Costagliola C, Mariano M, Andrea L, Napolitano P, D’Alessandro AG. Free Radical Properties, Source and Targets, Antioxidant Consumption and Health. Oxygen, 2022;2: 48–78. https://doi.org/10.3390/oxygen2020006
Minarti S, Junus M, Radiati LE, Jaya F, Fa'izah S, Landa F, Handayani I, Warasi DM, Fitriyono MA. Drying Methods Effect on Chemical Content of Pollen Study of Oil Palm Pollen as Honeybee Feed. Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021). Atlantis Press International, 2022;502–505
Mohammad SM, Mahmud-Ab-Rashid NK, Zawawi N. Stingless Bee-Collected Pollen (Bee Bread): Chemical and Microbiology Properties and Health Benefits. Molecules, 2021;26 (957):1–29 https://doi.org/10.3390/molecules26040957
Oyedapo O, Akinpelu B, Akinwunmi K, Adeyinka M, Sipeolu F. Red blood cell membrane stabilizing potentials. International Journal of Plant Physiology and Biochemistry, 2010;2: 46–5. http://www.academicjournals.org/ijppb
Özcan MM, Aljuhaim F, Babiker EE, Uslu N, Ceylan DA, Ghafoor K, Mete MÖ, et al. Determination of antioxidant activity, phenolic compound, mineral contents and fatty acid compositions of bee pollen grains collected from different locations. J. Apic. Sci, 2019;63:69–79. doi:10.2478/JAS-2019-0004
Ranieri A, Benelli G, Castagna A, Sgherri C, Signorini F, Bientinesi M, Nicolella C, Canale A. Freeze-drying duration influences the amino acid and rutin content in honeybee-collected chestnut pollen. Saudi Journal of Biological Sciences, 2017;26(2):252–255. https://doi.org/10.1016/j.sjbs.2017.08.011
Rao GHR. Metabolic Disease Pandemic: Call for Action. EC Endocrinology and Metabolic Research, 2020;5(9):1–3
Rzepecka-Stojko A, Drzal A, Stojko J, Buszman E. Effect of storage conditions on antioxidant activity of bee pollen extracts. Med. Weter, 2014;70(12):740–744
Saral Ö, Kilicarslan M, Sahin H, Yildiz O, Dincer B. Evaluation of antioxidant activity of bee products of different bee races in Turkey. Turkish Journal of Veterinary and Animal Sciences, 2019;43:41–447. doi:10.3906/vet-1901-3
Sari AM, Rosamah E, Suwinarti W, Kusuma IW, Arung ET. Aktivitas antioksidan dan antibakteri dari ekstrak bee pollen lebah kelulut (Tetragonula sarawakensis). Jurnal Riset Industri Hasil Hutan, 2021;13(2):123–132. http://dx.doi.org/10.24111/jrihh.v13i2.7050
Senthilmurugan G, Vasanthe VB, Suresh K. Screening and antibacterial activity analysis of some important medicinal plants. International Journal of Innovation and Applied Studies, 2013;2(2):146–152. http://www.issr-journals.org/ijias/
Singh H, Sarkar BK, Arya JC, Pal S, Kumar R, Gupta V, Singh R, Verma SC. Phytochemical and Anti-Inflammatory Evaluation of Herbal Gel Prepared from Bark Extract of Mesua Ferrea Linn. Journal of Drug Delivery and Therapeutics, 2019;9(5-s):53–56. http://dx.doi.org/10.22270/jddt.v9i5-s.3638
Spulber S, Raciti M, Dulko-Smith B, Lupu D, Rüegg J, Nam K, Ceccatelli S. Methylmercury interferes with glucocorticoid receptor: Potential role in the mediation of developmental neurotoxicity. Toxicology and Applied Pharmacology, 2018;1–7. https://doi.org/10.1016/j.taap.2018.02.021
Sukandar D, Nurbayt S, Rudiana T, Husna TW. Isolation and Structure Determination of Antioxidants Active Compounds from Ethyl Acetate Extract of Heartwood Namnam (Cynometra cauliflora L.).
Kimia Terap. Indonesia, 2017;19(1):11–17. http://inajac.lipi.go.id
Tutun H, Kaya MM, Usluer MS, Kahraman HA. Bee Pollen: Its Antioxidant Activity. U. Arı D. – U. Bee J, 2021;21(1):119–131. doi: 10.31467/uluaricilik.896045