İlker KEPENEKCİ, Tuba Kati ÇEKENGİL, Fatma Dolunay ERDOĞUŞ, Pervin ERDOĞAN, Hayriye Didem SAĞLAM

Beş Farklı Bitki Ekstraktının Domateste Zararlı Kök-Ur Nematod (Meloidogyne incognita Irk 2 ve M. arenaria Irk 2) (Tylenchida: Meloidoginidae)'larına Karşı Sera Koşullarındaki Etkisinin Belirlenmesi

Sebzelerin önemli zararlılarından biri de bitki köklerinde urlar meydana getirerek ekonomik değerde ürün kayıplarına neden olan kök-ur nematodları (Meloidogyne spp.) (KUN)'dır. Bu zararlı Türkiye’nin kıyı bölgeleri başta olmak üzere örtü altı sebze yetiştiriciliği yapılan tüm alanlarda yaygın olarak görülmektedir. Bu çalışmada, biber (Capsicum frutescens L.), ban otu (Hyoscyamus niger L.) (Solanaceae), pıtrak (Xanthium strumarium L.), civanperçemi (Achillea wilhelmsii C. Koch) (Asteraceae) ve tespih ağacı (Melia azedarach L.) (Meliaceae) bitkilerinden elde edilen ekstraktların 3 farklı konsantrasyonunun (%3, 6 ve 12) kök-ur nematod (Meloidogyne incognita ırk 2 ve M. arenaria ırk 2)'larına karşı etkileri araştırılmıştır. Çalışma kapsamında saksılara şaşırtılan domates (SC-2121 çeşidi) fidelerinin köklerine, M. incognita ve M. arenaria'ya ait 1000 larva (L2) ve 3000 yumurta ile birlikte bitkisel ekstraktlar uygulanmıştır. Denemeler Mart-Mayıs (2013) ayları arasında sera koşullarında yürütülmüştür. İnokulasyondan yaklaşık dokuz hafta sonra bitkiler sökülerek değerlendirmeler yapılmıştır. Denemeler sonunda; her bir bitki kökündeki kök-ur nematodlarına ait yumurta paketi sayısı, bitkinin boyu, bitkinin yaş ve kuru ağırlığı, kök yaş ve kuru ağırlığı parametreleri değerlendirilmiştir. Çalışma sonucunda M. arenaria’nin yumurta açılımına H. niger’in %12 (14,8±4,4) ve X. strumarium’un %12 (15±4,6)’lik konsantrasyonları etkili bulunmuş, M. incognita’nin yumurta açılımına ise X. strumarium’un %12 (11,4±5,1)’lik konsantrasyonu etkili olduğu belirlenmiştir. M. arenaria ve M. incognita’nin L2'lerine karşı M. azedarach’ın %12 (7,4±3,4 ve 7,4±2,5 sırasıyla)’lik konsantrasyonunun etkili olduğu tespit edilmiştir. Elde edilen veriler kullanılan bitki ekstraktlarının kök-ur nematodlarını baskılamada etkili olduğunu göstermiştir

Anahtar Kelimeler:

Kök-ur nematodu, Meloidogyne incognita, M. arenaria, bitkisel ekstraktlar, domates

The Effect of Five Different Plant Extracts on Root-Knot Nematodes [Meloidogyne incognita Race 2 and M. arenaria Race 2 (Tylenchida: Meloidoginidae)] Infesting Tomato under Greenhouse Conditions

Root-knot nematodes (Meloidogyne spp.) (RKNs) is one of the major pests of vegetables, which causes economic losses to vegetable production by bringing knots in the roots. RKNs are generally seen in the greenhouse vegetable growing areas of the coastal regions in Turkey. Herein, the effects of plant extracts (concentration of 3, 6 and 12%) from five different plant species; pepper (Capsicum frutescens L.), henbane (Hyoscyamus niger L.) (Solanaceae), common cocklebur (Xanthium strumarium L.), yarrow (Achillea wilhelmsii C. Koch) (Asteraceae) and bead-tree (Melia azedarah L.) (Meliaceae) were evaluated against the RKNs (Meloidogyne incognita race 2 and M. arenaria race 2). 1000 juvenile (J2) and 3000 eggs of M. incognita and M. arenaria were applied to the roots of tomato plants (SC-2121 variety) under greenhouse conditions. Plant extracts were applied at the same time with RKNs to the roots. Bioassay experiments were conducted during March to May (2013). The plants were pulled off 9 weeks after the application of plant extracts and assessed for the efficacy of the extracts. Different parameters such as total number of egg mass; fresh and dry root weight; fresh and dry weight of the above ground strata of the plants and plant height were measured at harvest. As a result, 12% of concentration H. niger (14,8±4,4) and 12% of X. strumarium (15±4,6) significantly suppressed the eggs of M. arenaria. The 12% concentration of X. strumarium (11,4±5,1) was found to be the most effective against M. incognita eggs. It was found that the 12% concentration of M. azedarach significantly suppressed the J2 of M. arenaria and M. incognita (7,4±3,4 and 7,4±2,5 respectively). The results showed that these plant extracts could be utilized for suppressing the RKNs used in this study

Keywords:

Root-knot nematode, Meloidogyne incognita, M. arenaria, plant extracts, tomato,

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Abbas S, Dawar S, Tariq M, Zaki MJ (2009). Nematicidal activity of spices against Meloidogyne javanica (Treub) Chitwood. Pakistan Journal of Botany, 41: 2625-2632.
Abbasi WM, Ahmed N, Zaki N, Shaukat SS (2008). Effect of Barleria acanthoides Vahl. on root-knot nematode infection and growth of infected okra and brinjal plants. Pakistan Journal of Botany, 40: 2193-2198.
Adegbite AA (2003). Comparative effects of Carbofuran and water extract of Chromolaena odorata on growth, yield and food components of root-knot nematode-infested soybean (Glycine max (L.) Merrill). University of Ibadan PhD Thesis, Nigeria.
Adegbite AA, Adesiyan OS (2005). Root extract of plant to control root-knot nematode on edible soybean. World Journal of Agricultural Sciences, 1: 18-21.
Ahmed S, Grainge M (1988). Handbook of Plants with Pest Control Properties. John Wiley & Sons Limited, New York, 470 pp.
Anonim (2010). Ruhsatlı Bitki Koruma Ürünleri, Tarım ve Köyişleri Bakanlığı, Koruma ve Kontrol Genel Müdürlüğü, Pulat Basımevi, Ankara, 398s.
Ardekani AS, Parhizkar S (2012). Inhibitory effects of Teucrium polium L., Artemisia sieberi Besser. and Achillea wilhelmsii C. Koch on Meloidogyne incognita (Kofoid and White) Chitwood (in vitro and under greenhouse conditions). Int. J. Med. Arom. Plants, 2: 596-602.
Bala SK, Bhattacharyya P, Mukherjee KS, Sukul NC (1986). Nematicidal properties of plant Xanthium strumarium L. and Parthenium histerophorus. Enviromental and Ecology, 4: 139-141.
Baytop T (1999). Türkçe Bitki Adları Sözlüğü. Türk Dil Kurumu Yayınları.
Bello LY, Chindo PS, Marley PS, Alegbejo MD (2006). Effects of some plant extracts on larval hatch of the rootknot nematode, Meloidogyne incognita. Archives of Phytopathology and Plant Protection, 39: 253-257.
Brauer M, Devkota B (1990). Control of Thaumatopoea piyocampa(Den.&Schiff) by extracts of Melia azedarach L.
(Meliaceae). Journal of Applied Entomology, 110: 128-135.
Chitwood DJ (2002). Phytochemical based strategies for nematode control. Annual Review of Phytopathology, 40: 221-249.
Çalmaşur Ö, Aslan İ, Şahin F (2006). Insecticidal and acaricidal effect of three Lamiaceae plant essential oils against Tetranychus urticae Koch and Bemisia tabaci.Genn. Industrial Crops and Products, 23: 140-146.
Çetinsoy S, Tamer A, Aydemir M (1998). Investigations on repellent and insecticidal effects of Xanthium strumarium L. on Colorado Potato Beetle Leptinotrsa decemlineata Say (Col: Chrysomelidae). Turkish Journal of Agriculture and Forestry, 22: 543-552.
Daykin ME, Hussey RS (1985). Staining and histopathological techniques in nematology. In: Barker, K.R., Carter, C.C., Sasser, J.N. (Eds.), An advanced treatise on Meloidogyne Volume II: Methodology. North Carolina State University Graphics, Raleigh, North Carolina, USA, 39-48 pp.
Douda O, Zouhar M, Mazakova J, Novakova E, Pavela R (2010). Using essences as alternative mean for northern root-knot nematode (Meloidogyne hapla) management. Journal of Pest Science, 83: 217-221.
Elekcioğlu İH, Uygun N (1994). Occurrence and distribution of plant parasitic nematodes in cash crops in eastern mediterranean region of Türkiye. Proc. of 9th Congress of the Mediterranean Phytopathological Union, 18-24
September 1994, Kuşadası-Aydın-Türkiye, pp. 409-410.
Erdoğan P, Toros S (2005). Melia azaderach L. (Meliaceae) ekstraktlarının Patates böceği [Leptinotarsa decemlineata Say (Col.:Chrysomelidae)] larvalarının gelişimi üzerine etkisi. Bitki Koruma Bülteni, 45: 99- 118.
Erdoğan P, Toros S (2007). Investigations on the effects of Xanthium strumarium L. extraracts on Colorado potato beetle [(Leptinotarsa decemlineata Say. Col.:Chrysomelidae)] Munis Entomology & Zoology, 2: 423-432.
Erdoğan P, Yıldırım A, Saltan G, Sever B (2010). Civanperçemi Achillea wilhelmsii C) Ekstraktının İki noktalı kırmızı örümcek [Tetranychus urticae Koch.(Arachnida: Tetranychidae)]’e Etkisi Üzerinde Araştırmalar.
Türkiye VI.Organik Tarım Sempozyumu Bildiriler Kitabı 70-75.Erdoğan P, Toros S (2013). Azadirachta indica A. Juss ile Melia azedarach L. Bitkilerinden Elde Edilen İnsektisitlerin Özellikleri ve Zararlılara Etkisi. Karaelmas Fen ve Mühendislik Dergisi, 3: 14-25.
Erdoğan P, Yıldırım A (2013). İki farklı bitki ekstraktının Yeşil şeftali yaprakbiti [(Myzus (N.) persicae Sulzer) (Hemiptera: Aphididae)]’ne insektisit etkileri üzerinde araştırmalar. Bitki Koruma Bülteni , 53: 33-42.
Erdoğan P, Yıldırım A (2015). Capsicum annuum L. (Solanaceae) ve Allium sativum L. (Amaryllidaceae) ekstraktlarının Myzus persicae (Sulzer) (Hemiptera:Aphididae) üzerine insektisit etkisi. Bitki Koruma Bülteni, 55: 305-315.
Erdoğan P, Yıldırım A (2016). Insecticidal Activity of Three Different Plant Extracts on the Green Peach Aphid [(Myzus persicae Sulzer) (Hemiptera: Aphididae)]. Journal Entomology Research Society, 8: 27-35.
Hackney RW, Dickerson OJ (1975). Marigold, castor bean, and chrysanthemum as control of Meloidogyne incognita and Pratylenchus alleni. Journal of Nematology, 7: 84-90.
Hasabo SA, Noweer EMA (2005). Management of Root-Knot Nematode Meloidogyne incognita on Eggplant with some plant extracts. Egyptian Journal of Phytopathology, 33: 65-72.
Hussey RS, Barker KR (1973). A comparison of methods of collecting inocula of Meloidogyne spp., including a new technique. Plant Disease Reporter, 57: 1025-1028.
Javed N, Anwar SA, Inam-ul-haq M, Ahmad R, Khan HU (2006a). Effect of neem formulations applied as soil drenching on invasion and development of root-knot nematode, Meloidogyne javanica. In: Proceeding of International Symposium on Sustainable Crop Improvement and Integrated Management. University of Agriculture, 14-16 September 2006, Faisalabad, Pakistan. 244-247pp.
Javed N, Gowen SR, Inam-ul-haq M, Abdullah K, Shahina F (2006b). Systemic and persistent effect of neem(Azadirachta indica) formulations against root-knot nematodes, Meloidigyne javanica and their storage life. Crop Protection, 26: 911-916.
Kepenekci I, Saglam HD (2015). Extracts of some indigenous plants affecting hatching and mortality in the root-knot nematode [Meloidogyne javanica (Treub) Chitwood]. Egyptian Journal of Biological Pest Control, 25: 39-44.
Kepenekci I, Erdoğuş D, Erdoğan P (2016). Effects of some plant extracts on root-knot nematodes in vitro and in vivo conditions. Turkish Journal of Entomology, 40: 1-12.
Lee MJ (1990). The effect of extracts of Melia azedarach on Meloidogyne incognita. Quarterly Journal of Chinese Forestry, 20: 1-7.
Malik MSN, Sanfwan K, Bahatti KSDS (1988). Nematicidal activity of exracts of Xanthium strumarium L. Weeb Abstract 37: 16733p.
Melakeberhan H (1997). Effect of temperature and nitrojen source on tomato genotypes responce to Meloidogyne incognita infection. Fundamental and applied nematology, 20: 1-8.
Melakeberhan H, Jones AL, Bırd GW (2000). Effects of soil pH and Pratylenchus penetrans on the mortality of “Mazzard” cherry seedlins and their susceptibility to Pseudomonas syringae pv syringae. Canadian Journal Plant Pathology, 22: 131-137.
Mennan S, Ecevit O, Mennan H (2000). Bazı bitki ekstraktlarının kök-ur nematodu (Meloidogyne incognita) (Konfoid ve White, 1919)’na nematisit etkilerinini araştırılması. Türkiye Herboloji Dergisi, 3: 1-9.
Mohammad AB, Eskandar Z, Saeid S, Mohammad J, Fariba M (2007). Evaluation of sulfosulfuran for broadleaved and grass weed control in wheat (L.) in Iran. Crop Protection, 26: 1385-1389.
Nakasone KK, Peterson SW, Jong SC (2004). Preservation and Distribution of Fungal Cultures. In: Mueller G.M., Bills G.F. and Foster M.S. (eds) Biodiversity of fungi, Inventory and Monitoring Methods. Elsevier Academic Press pp. 47-37.
Nandal SN, Bhatti DS (1986). The effect of certain edaphic factors on the nematicidal activity of plant extracts. Nematologia Mediterrana, 14: 295-298.
Netscher C, Sikora RA (1990). Nematode parasites of vegetables. In Plant Parasitic nematodes in Subtropical and Tropical Agriculture. Edited by M. Luc, RA. Sikora and J. Bridge. CAB International, Wellingford, U.K. pp. 237-284.
Oka Y, Nacar S, Putievsky E, Ravid U, Yaniv Z, Spiegel Y (2000). Nematicidal activity of essential oils and their components against the root-knot nematodes. Journal of Phytopathology, 90: 710-715.
Orisajo SB, Okeniyi MO, Fademi OA, Dongo LN (2007). Nematicidal effects of water extracts of Acalypha ciliate, Jatropha gosssypifolia, Azadiractha indica and Allium ascalonicum on Meloidogyne incognita infection on cacao seedlings. Journal of Research in Biosciences, 3: 49-53.
Öncüer C (2000). Tarımsal zararlılarla savaş yöntem ve ilaçları (4. Baskı). Adnan Menderes Üniversitesi Yayınları. No:13, 333 s.
Prakash A, Rao J (1996). Botanical Pesticides in Agriculture. CRC Pres, Lewis Publishers, 461 pp
Rehman B, Ganai MA, Siddiqui KPMA, Usman A (2012). Management of Root Knot Nematode, Meloidogyne incognita Affecting Chickpea, Cicer arientinum for Sustainable Production. Biosciences International, 1:1-5.
Sasser JN, Carter CC (1985). An Advanced Treatise on Meloidogyne. Vols 1 and 2. North Carolina State University, Raleigh, North CarolinaShanker C, Solanki KR (2000). Botanical insecticides: A historical perspective. India, Asian Agrihistory, 4: 21-30.
Shaukat SS, Siddiqui IA (2001). Nematicidal activity of some weed extracts against Meloidogyna javanica (Treub.) Chitwood. Pakistan Journal of Biological Sciences, 4: 1251-1252.
SPSS I (2012). Statistics for windows, version 21.0. IBM Corp., Armonk, NY. Taba S, Sawada J, Moromizato Z (2008). Nematicidal activity of Okinawa Island plants on the root-knot nematode Meloidogyne incognita (Kofoid and White) Chitwood. Plant Soil, 303: 207-216.
Tariq M, Dawar S, Mehdi FS, Zaki MJ (2006). Use of Avicennia marina (Forsk.) Vierh in the Control of Root Knot Nematode Meloidogyne javanica (Treub) Chitwood on Okra and Mash Bean. Turkish Journal of Biology, 31: 225-230.
Taylor DP, Netscher C (1974). An improved technique for preparing perineal patterns of Meloidogyne spp. Nematologica, 20: 268-269.
Tsay TT, Wu ST, Lin YY (2004). Evaluation of Asteraceae Plants for Control of Meloidogyne incognita. Journal of Nematology, 36: 36-41.
Yelekçi K, Acımış M, Soran H (1981). Melia azedarach L. meyvelerinden çıkarılan özütlerin çam keseböceği Thaumetopoea pityocampa Schiff (Lepidoptera: Thaumetopoeidae) Tırtıllarına Etkisi. Doğa Bilim Dergisi, Temel Bilim, 5: 69-71.