Vitex agnus-castus L. (Verbenaceae) uçucu yağının kimyasal bileşeni ve Thaumetopoea pityocampa (Denis & Schiffermüller, 1775) (Lepidoptera: Notodontidae) larvaları üzerindeki larvasidal etkinliği

Bu çalışmada, hayıt (Vitex agnus-castus L.) (Verbenaceae) bitkisinden elde edilen uçucu yağın Gaz Kromatografisi-Kütle Spektrometresi (GC-MS) yöntemiyle kimyasal bileşeni analiz edilmiştir ve onun 250, 500 ve 1000 μL/L dozlarında 24., 48., 72. ve 96. saatlerde uygulanmasıyla, çam keseböceğinin (Thaumetopoea pityocampa (Denis & Schiffermüller, 1775) (Lepidoptera: Notodontidae)) beş farklı dönem larvalarına karşı larvasidal etkinliği araştırılmıştır. Bu çalışma, 2015-2017 yılları arasında Muğla Sıtkı Koçman Üniversitesi’nde (Fethiye A.S.M.K. Meslek Yüksekokulu’nda) laboratuvar şartlarında (25±2ºC), %65±5 ON ve 14/10 A/K) yürütülmüştür. Her bir larvasidal etkinlilik testi üç kez tekrarlanmıştır. β-caryophyllene (%16,8), germacrene D (%14,7), 1,8-cineole (%14,5) ve ζ-gurjunene (%11,8) bu uçucu yağın ana bileşenleri olarak kaydedilmiştir. Çalışmanın 96. saatinde, uçucu yağ %13,30 ile %96,6 arasında ölümlere sebep olmuştur. 250, 500 ve 1000 μL/L dozlarındaki en yüksek ölüm oranları sırasıyla, L1 dönemi için %70,0, L3 dönemi için %80,0 ve L2, L3, L4 dönemleri için ise %96,6 olarak kaydedilmiştir. Bütün bunlar göz önüne alındığında, V. agnus-castus uçucu yağının kontrollerle kıyaslandığında, T. pityocampa larvalarının kontrolü için potansiyel bir alternatif olduğu sonucuna varılabilir.

Chemical composition of Vitex agnus-castus L. (Verbenaceae) essential oil and its larvicidal effectiveness on Thaumetopoea pityocampa (Denis & Schiffermüller, 1775) (Lepidoptera: Notodontidae) larvae

In this study, the chemical composition of the essential oil obtained from chaste tree (Vitex agnus-castus L.) (Verbenaceae) was analyzed by gas chromatography-mass spectrometry (GC-MS) and its larvicidal effectiveness at 250, 500 and 1000 μL/L, and 24, 48, 72 and 96 h against the five instars of the pine processionary moth, Thaumetopoea pityocampa (Denis & Schiffermüller, 1775) (Lepidoptera: Notodontidae) investigated. The study was conducted at Muğla Sıtkı Koçman University (Fethiye A.S.M.K. Vocational High School) under laboratory conditions (25±2ºC, 65±5% RH and 14:10 h L:D photoperiod) in 2015-2017. Each larvicidal effectiveness test was repeated three times. β-caryophyllene (16.8%), germacrene D (14.7%), 1,8-cineole (14.5%) and ζ-gurjunene (11.8%) were identified as main compounds of the essential oil. Ninety-six h after treatment, the essential oil caused between 13.3 and 96.6% mortality. The highest mortality at 250, 500 and 1000 μL/L doses were 70.0% for L1, 80.0% for L3 and 96.6% for L1, L3 and L4 instars after 96 h, respectively. It was concluded that the V. agnus-castus essential oil has potential as an alternative for control of T. pityocampa larvae.

Keywords:

Chaste tree, essential oil pine processionary moth, toxicity,

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