Fahriye Sümer ERCAN, Sevcan ÖZTEMİZ, Aydın Suzu Tunçbilek (195-201)

Trichogramma euproctidis Girault ve Trichogramma brassicae Bezdenko (Hymenoptera:Trichogrammatidae)'nin ayırımında mitokondriyal ve ribozomal DNA dizi analizi

Ribosomal bölgeler dizi varyasyonu: nuclear ribosomal gen dizisinin ikinci genler arası bölgesi (ITS2), 28S ribosomal altünitenin D2 bölgesi (28SD2) ve mitokondrial sitokrom oksidaz I (COI) Trichogramma euproctidis Girault ve Trichogramma brassicae Bezdenko (Hymenoptera: Trichogrammatidae) popülasyonlarının ayırımı için incelenmiştir. ITS2 bölgesinin uzunluğu 378 bç ile 406 bç arasında değişmiştir. 28SD2 ve COI dizilerinin boyutu sırasıyla T. euproctidis için 522 bç ile 669 bç ve T. brassicae için 530 bç ile 672 bç olarak farklı bulunmuştur. Filogenetik ilişki her bir marker için maximum likelihood yöntemi kullanılarak oluşturulmuştur. ITS2 gen bölgesi, T. euproctidis ve T. brassicae’yi 28SD2 ve COI genlerinden daha net ayırt etmiştir.

Anahtar Kelimeler:

Trichogramma euproctidis, Trichogramma brassicae, ITS2, 28SD2, COI

Mitochondrial and ribosomal DNA sequence analysis for discrimination of Trichogramma euproctidis Girault and Trichogramma brassicae Bezdenko (Hymenoptera: Trichogrammatidae)

Sequence variation of ribosomal regions, namely the second internal transcribed spacer region of the nuclear ribosomal gene cluster (ITS2), the D2 domain of 28S ribosomal subunit (28SD2), and the mitochondrial cytochrome oxidase I (COI) were examined for differentiation of Trichogramma euproctidis Girault and Trichogramma brassicae Bezdenko (Hymenoptera: Trichogrammatidae) populations. The length of ITS2 region ranged from 378 bp to 406 bp. The sequences of 28SD2 and COI were 522 bp and 669 bp for T. euproctidis and 530 bp and 672 bp for T. brassicae, respectively. A phylogenetic relationship was constructed by using the maximum likelihood method for each marker. The ITS2 gene region more clearly differentiated between T. euproctidis and T. brassicae than the 28SD2 and COI genes.

Keywords:

Trichogramma euproctidis, Trichogramma brassicae, ITS2, 28SD2, COI,

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Cruickshank, R.H., 2002. Molecular markers for the phylogenetics of mites and ticks. Systematic & Applied Acarology, 7: 3-14.
Doganlar, O., M. Doganlar & A. Frary, 2010. The current status of thrips parasitoids Ceranisus (Hymenoptera: Eulophidae) species in Turkey based on morphology and 28S D2 rDNA. Turkish Journal of Entomology, 34 (1): 89-104.
Ercan, F.S., S. Oztemiz, A.S. Tuncbilek & R. Stouthamer, 2011. Sequence analysis of the ribosomal DNA ITS2 region in two Trichogramma species (Hymenoptera: Trichogrammatidae). Archives of Biological Sciences, 63 (4): 949-954.
Gillespie, J.J., J.B. Munro, J.M. Heraty, M.J. Yoder, A.K. Owen & A.E. Carmichael, 2005. A secondary structural model of the 28S rRNA expansion segments D2 and D3 for Chalcidoid wasps (Hymenoptera: Chalcidoidea). Molecular Biology and Evolution, 22(7): 1593-1608.
Hall, T.A., 1999. BioEdit: a user-friendly biological sequence alignment [ed.], and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41: 95-98.
Karimi, J., R. Darsouei, M. Hosseini & R. Stouthamer, 2012. Molecular characterization of Iranian Trichogrammatids (Hymenoptera: Trichogrammatidae) and their Wolbachia endosymbiont. Journal of Asia-Pacific Entomology, 15: 73-77.
Landry, B.S., L. Dextraze & G. Boivin, 1993. Random amplified polymorphic DNA markers for DNA fingerprinting and genetic variability assessment of minute parasitic wasp species (Hymenoptera: Mymaridae and Trichogrammatidae) used in biological control programs of phytophagous insects. Genome, 36: 580-587. Mardulyn, P. & J.B. Whitfield, 1999. Phylogenetic Signal in the COI, 16S, and 28S genes for inferring relationships among genera of Microgastrinae (Hymenoptera; Braconidae): Evidence of a high diversification rate in this group of parasitoids. Molecular Phylogenetics and Evolution. 12(3): 282-294.
Nagarkatti, S. & H. Nagaraja, 1971. Redescriptions of some known species of Trichogramma (Hym., Trichogrammatidae), showing the importance of the male genitalia as a diagnostic character. Bulletin of Entomological Research, 61(1): 13-31.
Oztemiz, S., 2007. Trichogramma species egg parasitoids of Lepidoptera in the Eastern Mediterranean Region of Turkey. Proceedings of the Entomological Society of Washington, 109: 718-720.
Polaszek, A., P.F. Rugman-Jones, R. Stouthamer, E. Hernandez-Suarez, T. Cabello, & M.P. Pe´rez, 2012. Molecular and morphological diagnoses of five species of Trichogramma: biological control agents of Chrysodeixis chalcites (Lepidoptera: Noctuidae) and Tuta absoluta (Lepidoptera: Gelechiidae) in the Canary Islands. BioControl, 57: 21-35.
Rugman-Jones, P.F., R. Wharton, T. Noort, & R. Stouthamer, 2009. Molecular differentiation of the Psyttalia concolor (Szépligeti) species complex (Hymenoptera: Braconidae) associated with olive fly, Bactrocera oleae (Rossi) (Diptera: Tephritidae), in Africa, Biological Control, 49: 17-26.
Rugman-Jones, P.F., M.S. Hoddle, & R. Stouthamer, 2010. Nuclear-mitochondrial barcoding exposes the global pest western flower thrips (Thysanoptera: Thripidae) as two sympatric cryptic species in it’s native California. Journal of Economic Entomology, 103(3): 877- 886.
Sayed, S.M., A.M. El-Shehawi & S.A. Al-Otaibi, 2011. Molecular and biological characterization of Trichogramma turkestanica (Hymenoptera: Trichogrammatidae) which inhabits Taif governorate at the west of Saudi Arabia, African Journal of Biotechnology, 10 (46): 9467-9472.
Smith, S.M., 1996. Biological control with Trichogramma: advances, successes, and potential of their use. Annual Review of Entomology, 41: 375-406.
Stouthamer, R., R.F. Luck & W.D. Hamilton, 1990. Antibiotics cause parthenogenetic Trichogramma (Hymenoptera: Trichogrammatidae) to revert to sex. Proceedings of the National Academy of Sciences, 87: 2424-2427. Stouthamer, R., H. Jianguo, F.J.P.M. van Kan, G.R. Platner, & J.D. Pinto. 1999. The utility of internally transcribed spacer 2 DNA sequences of the nuclear ribosomal gene for distinguishing sibling species of Trichogramma. BioControl, 43: 421-440.
Stouthamer R., Y. Gai, A.B. Koopmanschap, J.D. Pinto, & G.R. Platner, 2000. ITS-2 sequences do not differ for the closely related species Trichogramma minutum and T. platneri. Entomologia Experimentalis et Applicata, 95: 105Tamura, K, J. Dudley, M. Nei & S. Kumar, 2007. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24: 1596-1599.
Vickerman, D.B., M.S. Hoddle, S. Triapitsyn & R. Stouthamer, 2004. Species identity of geographically distinct populations of the glassy-winged sharpshooter parasitoid Gonatocerus ashmeadi: morphology, DNA sequences, and reproductive compatibility, Biological Control, 31: 338-345.
Wagener, B., A. Reineke, B. Löhr & C.P.W. Zebitz, 2006. Phylogenetic study of Diadegma species (Hymenoptera: Ichneumonidae) inferred from analysis of mitochondrial and nuclear DNA sequences. Biological Control, 37: 131-1 A