Hasan Sungur CİVELEK, Alper TONGUÇ, Bekir ÇÖL, Betül KAYA, Okan ÖZGÜL

The use of RAPD-PCR analysis in characterization of Liriomyza congesta (Becker 1903), Agromyza apfelbecki strobl,1902 and Chromatomyia horticola (Goureau, 1851) species collected from Turkey

Liriomyza trifolii (Burgess, 1880), Liriomyza congesta, (Becker, 1903) Agromyza apfelbecki Strobl, 1902 ve Chromatomyia horticola (Goureau, 1851) türleri Türkiye’nin tarımsal alanlarında önemli sebze zararlılarındandır. Bu türler morfolojik karakterleri ile kolaylıkla ayırt edilemezler. Bu çalışmanın amacı, bu türleri kesin bir şekilde ayırt edebilmek için genetik işaretleyicileri oluşturmak ve Türkiye’deki değişik coğrafik alanlardan toplanan L. trifolii bireyleri arasındaki genetik varyasyonları kavramaktır. Böylece 4 rastgele primer kullanılarak RAPD işaretleyicileri çalıştırılmıştır. Farklı RAPD profilleri farklı türlerin belirlenmesinde kullanılmış ve böylece RAPD-PCR analizlerinin, klasik metotlar yoluyla rutin bir şekilde ele alınarak bu türlerin ayırt edilmesine yardımcı olma doğrultusunda hızlı bir tarama için kullanışlı bir araç olarak başvurulabileceğini göstermiştir. Sebze ürünlerine zarar vererek önemli ekonomik kayıplara neden olduğundan dolayı bu zararlının yayılımını önlemeye yönelik kontrol stratejilerinin geliştirilmesi için bu türlerin biyotiplerinin tanımlanması önemlidir. Bu çalışmadan elde edilen sonuçlar RAPD’in, türlerin ve ırklarının geleneksel teşhislerine önemli bir alternatif olarak umut verici bir şekilde etkili, hızlı ve ekonomik bir yöntem olduğunu, göstermiştir.

Türkiye 'den toplanan Liriomyza trifolii (Burgess,1880), L.congesta (Becker,1903), Agromyza apfelbecki Strobl,1902 ve chromatomyia horticola (Goureau,1851) türlerinin karakterize edilmesinde RAPD-PCR analizinin kullanımı

The Liriomyza trifolii (Burgess, 1880), Liriomya congesta (Becker, 1903), Agromyza apfelbecki Strobl, 1902 and Chromatomyia horticola (Goureau, 1851) species have become important vegetable pests in the agricultural areas in Turkey. These species are not easily differentiated by their morphological characteristics. The aim of the present study was to obtain genetic markers to unambiguously distinguish these species and gain insight into genetic variation between the individuals of L. trifolii strains collected from various geographic locations in Turkey. Thus, four random primers were employed to generate RAPD markers. Different RAPD profiles were observed for the different species indicating that the RAPD-PCR analysis can be applied as a useful tool in quickly screening the strains to aid in discriminating these species that was routinely done via classical methods. Identification of biotypes of the species is crucial in order to designing control strategies to avoid the spread of the pests because of economic losses caused by the damages to vegetable crops. Our results show that RAPD is promisingly an effective, fast and economic way, hence proposed as a valuable alternative to traditional identification of the insect species and strains.

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