Molecular diagnosis of Globodera rostochiensis Wollenweber, (Tylenchida: Heteroderidae) in the potato growing areas of Aegean Region, Turkey

Ege Bölgesi’nde patates alanlarından 2006-2008 yıllarında 8 adet kist nematodu populasyonu toplanarak, elde edilen kistlerden DNA izolasyonu yapılmıştır. Türe özgü spesifik primerler ile gerçekleştirilen Multipleks PCR sonucunda Globodera rostochiensis Wollenweber, (Tylenchida: Heteroderidae)’e özgü 435 bp de bant oluşumu gözlenmiştir. Aynı örneklerin rDNA2 ve rDNA1.58S primerleri ile PCR yapılarak Hinf l enzimi kesimi sonucunda G. rostochiensis’e özgü 233bp ve 522bp.de bant verdiği tespit edilmiştir. Bu çalışmada patates kist nematodlarından Globodera pallida Stone, 1973 tespit edilmemiştir. Türkiye’de daha önce morfometrik yöntemlerle saptanan, G. rostochiensis’ in varlığı moleküler yöntemlerle de ilk defa belirlenmiştir

Ege Bölgesi patates alanlarında Globodera rostochiensis Wollenweber, (Tylenchida: Heteroderidae)’in moleküler yöntemlerle saptanması

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Türkiye Entomoloji Dergisi-Cover
  • ISSN: 1010-6960
  • Başlangıç: 1977
  • Yayıncı: Galip KAŞKAVALCI
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