Halil TOKTAY, Mustafa İMREN, İ. Halil ELEKÇİOĞLU, Lieven WAEYENBERGE, Amer DABABAT, Nicole VIAENE

Molecular characterization of cereal cyst nematodes from the South Anatolian Region in Turkey using ITS-rDNA sequences

Tahıl kist nematodlarının (Heterodera avenae group) buğday köklerinde beslenen 12 farklı türü bilinmektedir. Türkiyede buğday yetiştiriciliğinin yapıldığı farklı alanlarda ekonomik olarak önemli üç tür, Heterodera avenae Wollenweber, 1924, Heterodera filipjevi (Madzhidov, 1981) Stelter, 1984 ve Heterodera latipons Franklin, 1969 bulunmaktadır. Bu çalışmada Güneydoğu Anadolu Bölgesi (Hatay, Kahramanmaraş, Gaziantep, Kilis ve Mardin) buğday alanlarından toplanan 47 adet Tahıl kist nematodu popülasyonuna ait ribozomal DNAnın transkripte olmayan (ITS-rDNA) bölgesi sekanslanarak moleküler teşhisi yapılmıştır. Filogenetik analiz sonuçlarına göre, H. avenae, H. filipjevi, H. latipons ve H. ciceri Vovlas, Greco & Di Vito, 1985 tanımlanmıştır. Çalışmada kullanılan nematod popülasyonunun % 76,5i H. latipons, %13ü H. filipjevi, % 8,5i H. avenae ve % 2si Heterodera ciceri olarak saptanmıştır.

Güney Anadolu Bölgesi Tahıl kist nematodu popülasyonlarının ribozomal DNA nın ITS bölgesi sekanslanarak moleküler karakterizasyonu

The Heterodera avenae group includes 12 species feeding on roots of cereals. Three species, Heterodera avenae Wollenweber, 1924, Heterodera filipjevi (Madzhidov, 1981) Stelter, 1984 and Heterodera latipons Franklin, 1969 are among the most economically important cyst nematode pests of cereals cultivated in different parts of Turkey. In this study, forty seven cereal cyst nematode isolates collected from cereal growing areas of the South Anatolian Region of Turkey (Hatay, Kahramanmaraş, Gaziantep, Kilis, Mardin and Adıyaman), were identified by using sequence analysis of the Internal Transcribed Spacer region of the ribosomal DNA (ITS-rDNA). Based on phylogenetic analysis using ITS-rDNA sequences, H. avenae, H. filipjevi, H. latipons and Heterodera ciceri Vovlas, Greco & Di Vito, 1985 were identified. 76,5 % of the isolates were characterized as H. latipons, 13 % H. filipjevi, 8,5 % H. avenae and 2 % H. ciceri.

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Abidou, H., A. El-Ahmed, J. M. Nicol, N. Bolat, R. Rivoal & A. Yahyaoui, 2005. Occurrence and distribution of species of the Heterodera avenae group in Syria and Turkey. Nematology Mediterranean, 33: 197-203.
Bekal, S., J. P. Gauthier & R. Rivoal, 1997. Genetic diversity among a complex of cereal cyst nematodes inferred from RFLP analysis of the ribosomal internal transcribed spacer region. Genome, 40: 479486.
Ferris, V. R., J. M. Ferris, J. FaghihI & M. Ireholm, 1994. Comparisons of isolates of Heterodera avenae using 2-D PAGE protein patterns and ribosomal DNA. Journal of Nematology, 26: 144-151.
İmren, M., A. Özarslandan, H. Toktay, A. Öcal, İ. H. Elekçioğlu, A. Dababat & J. Nicol, 2010. Preliminary survey study on Heterodera species in some provinces of Southeast Anatolia region of Turkey (19-23 September, Vienna, 2010). Proc. of 30th European Society of Nematologist, 68 pp.
İmren, M., H. Toktay, A. Özarslandan, A. Öcal & İ. H. Elekçioğlu, 2011. Doğu Akdeniz Bölgesi buğday alanlarında Tahıl kist nematodu (Heterodera avenae group) türlerinin belirlenmesi. Türkiye IV. Bitki Koruma Kongresi 28- 30 Haziran 2011- Kahramanmaraş. s 10.
Kort, J., 1960. A technique for the extraction of Heterodera cysts from wet soil and for the estimation of their egg and larval content. Verslagen en Medelingen Plantenziektenkundige Dienst. Wageningen. Netherlands, 233: 3-7.
Madani, M., N. Vovlas, P. Castillo, S. A. Subbotin & M. Moens, 2004. Molecular characterization of cyst nematode species (Heterodera spp.) from the Mediterranean basin using RFLPs and sequences of ITS-rDNA. Journal of Phytopathology, 152: 229-234.
Nicol J. M., 2002. Important nematode pests. Pp. 345366 in B. C. Curtis, S. Rajaram, and H. Gomez Macpherson, eds. Bread wheat: Improvement and production. Rome: FAO Publisher.
Nicol, J., R. Rivoal, S. Taylor & M. Zaharieva, 2004. Global Importance of Cyst (Heterodera spp.) and Lesion Nematodes (Pratylenchus spp.) on Cereals: yield loss, population dynamics, use of host resistance and integration of molecular tools. Nematology Monographs and Perspectives 2: 233-251.
Nicol J. M., N. Bolat, A. Bağcı, R. T. Trethowan, M. William, H. Hekimhan, A. F. Yıldırım, E. Şahin, İ. H. Elekcioğlu, H. Toktay, B. Tunalı, A. Hede, S. Taner, H. J. Braun, M. Van Ginkel, M. Keser, Z. Arısoy, A. Yorgancılar, A. Tülek, D. Erdurmuş, O. Büyük & M. Aydoğdu, 2005. The international breeding strategy for the incorporation of resistance in bread wheat against the soil borne pathogens (Dryland Root Rot and Cyst and Lesion Nematodes) Using Conventional and Molecular Tools. 7th International Wheat Congress, 2005, Mardel Plata, Argentina.
Nicol J. M & R. Rivoal, 2008. Global knowledge and its application for the integrated control and management of nematodes on wheat. In Integrated management and biocontrol of vegetable and grain crops nematodes. (Eds A Ciancio, KG Mukerji) pp. 243-287. (Springer Academic Publishing: The Netherlands)
Rivoal R., S. Valette, S. Bekal, J. P. Gauthier & A. Yahyaoui, 2003. Genetic and phenotypic diversity in the graminaceous cyst nematode complex, inferred from PCR-RFLP of ribosomal DNA and morphometric analysis. European Journal of Plant Pathology, 109: 227241.
Smiley R. W, G. P Yan & Z. A. Handoo, 2008. First record of the cyst nematode Heterodera filipjevi on wheat in Oregon. Plant Disease, 92: 1136.
Subbotin, S. A., L. Waeyenberge, I. A. Molokanova & M. Moens, 1999. Identification of Heterodera avenae group species by mophometrics and rDNA-RFLPs. Nematology, 1: 195-207.
Subbotin, S. A., L. Waeyenberge & M. Moens, 2000. Identification of cyst forming nematodes of the genus Heterodera (Nematoda: Heteroderidae) based on the ribosomal DNA-RFLP. Nematology, 2: 153-164.
Subbotin, S. A., D. Sturhan, H J. Rumpenhorst & M. Moens, 2003. Molecular and morphological characterization of the Heterodera avenae species complex (Tylenchida: Heteroderidae). Nematology 5: 515-538.
Şahin, E., J. M. Nicol, İ. H. Elekcioğlu, Ö. Yorgancılar, A. F. Yıldırım, A. Tülek, H. Hekimhan, A. Yorgancılar, A. T. Kılınç, N. Bolat & G. Erginbaş-Orakçı, 2009. Frequency and diversity of cereal nematodes on the Central Anatolian Plateau of Turkey. In Cereal cyst nematodes: status, research and outlook. (Eds IT. Riley, J. M. Nicol, A. A. Dababat) pp. 100-105.
Tamura K., D. Peterson, N. Peterson, G. Stecher, M. Nei & S. Kumar, 2011. MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution (In Press).
Tanha, M.T., S. A. Subbotin, M. Moens., 2003. Molecular identification of cyst-forming nematodes (Heteroderidae) from Iran and a phylogeny based on ITS-rDNA sequences. Nematology 5, 99111.
Thompson, J. D., T. J. Gibson, F. Plewniak, F. Jeanmougin & D. G. Higgins, 1997. The Clustal X Windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25: 4876-4882.
Vrain, T. C., D. A. Wakarchuk, A. C. Le´vesque & R. I. Hamilton, 1992. Intraspecific rDNA restiction fragment length polymorphism in the Xiphinema americanum group. Fundamental and Applied Nematology, 15: 563573.
Yüksel, H., 1973. Studies on the morphological and biological differences of Heterodera species (Nematoda: Heteroderidae) in Turkey. Atatürk Üniversity Ziraat Fakültesi Dergisi 4, pp. 5371.
Wendt, K. R., T. C. Vrain & J. M. Webster, 1993. Separation of three species of Ditylenchus and some host races of D. dipsaci by restriction fragment length polymorphism. Journal of Nematology, 25: 555563.
Wouts, W. M & D. Sturhan, 1995. Heterodera aucklandica sp. n. (Nematoda: Heteroderidae) from a New Zealand native grass, with notes on the species of the H. avenae group. New Zealand. Journal of Zoology 22: 199-207.
Zijlstra, C., J. B. Uenk & C. H. Silfhout, 1997. A reliable, precise method to differentiate species of root-knot nematodes in mixtures on the basis of ITS-RFLPs. Fundamental and Applied Nematology, 20: 5963.