Farklı bitki ekstraktlarının Spodoptera littoralis (Lepidoptera: Noctuidae) üzerinde beslenme engelleyici ve mide zehiri etkileri

Bu çalışmada 9 farklı bitkiden elde edilen bitki ekstraktının Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae) üzerindeki beslenmeyi engelleyici ve mide-zehiri etkileri araştırılmıştır. Denemelerde kullanılan ekstraktlar 2 1 mg/cm arasında en yüksek beslenmeyi engelleyici etki sırasıyla Delphinium consolida L. (% 68.96) Chrysanthemum segetum L. (% 62.67), Artemisia vulgaris L. (% 61.09) ve Tanacetum mucroniferum Hub.Mor.& Grierson, (% 55.87) bitkilerinden elde edilmiştir. Bu dört bitki ile yürütülen doz etki çalışmalarında uygulama dozu 2 2 mg/cm ’ye çıkarıldığında bitki ekstraktları % 68.91 ile % 82.20 arasında beslenmeyi engelleyici etki göstermiştir. 0.5 2 mg/cm dozda ise D. consolida aktivitesi % 48.92 T. mucreniferum aktivitesi ise % 45.02 olarak belirlenmiştir. Mide zehiri etki çalışmalarında 120 saat sonunda en yüksek aktivite % 74.24 ölüm oranı ile C. segetum ekstraktında belirlenmiş bu ekstraktı % 70.53 ve % 67.04 ölüm oranlarıyla D. consolida ve T. mucroniferum ekstraktları takip etmiştir. Mide zehiri etkisi çalışmalarında yüksek aktivite gösteren bitkilerle yapılan doz-ölüm çalışmasında en yüksek2 2toksisite 0.35 mg/cm LC50 değeri ve 3.85 mg/cm LC90 değeri ile C. segetum ekstraktında saptanmıştır.

Antifeedant and stomach poison effects of different plant extracts to Spodoptera littoralis (Lepidoptera: Noctuidae)

Antifeedant and stomach poison effects of 9 plant extracts were tested on Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae). Antifeedant effects of plant extracts varied from 22.44% to 68.96% and the most active plant extracts were Delphinium consolida (%68.96), Chrysanthemum segetum (62.67%), Artemisia vulgaris (61.09%) and Tanacetum mucroniferum (55.87%). Dose-response studies with the extracts showed that all extracts produced 2 strong antifeedant effects ranging from 68.91% to 82.20% at 2 mg/cm dose, D. consolida (48.92%) and T. mucroniferum (45.02%) also exhibited antifeedant properties at dose as low as 0.5 mg/cm2. In stomach posion effects studies, C. segetum was the most toxic extract with %74.24 mortality. It was followed by D. consolida and T. mucroniferum with 70.53% and 67.04% mortality, respectively. Dose-mortality studies with C. segetum showed that it 2 2 was the most toxic extract with 0.35 mg/cm LC50 and 3.85 mg/cm LC90 values.

PDF

___

Abbott, W.S., 1925. A metod for computing the effectivenes of an insecticide. Journal of Economic Entomology, 34: 1426-1432.
Alkan, M. & A. Gökçe, 2012. Tanacetum abrotanifolium (L.) ekstraktlarının Sitophilus oryzae ve Sitophilus granarius (Coleoptera: Curculionidae)’a uzaklaştırıcı ve beslenmeyi engelleyici etkileri. Türkiye Entomoloji Dergisi, 36(3): 377-389.
Aydın, H., 2002. Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae)’e Karşı Bazı Tanacetum spp. (Compositae) Ekstraktlarının Biyolojik Aktivitelerinin Belirlenmesi. Ankara Üniversitesi, Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi, Ankara, 100 s.
Cornelius, W.W., T. Akenga, G.O. Obiero, & K.P. Lutta, 2009. Antifeedant activities of the erythrinaline alkoloids from Erythrina latissima against Spodoptera littoralis (Lepidoptera noctuidae). Records of Natural Products, 3 (2): 96-103.
Dayan, F.E., C.L. Cantrell & S.O. Duke, 2009. Natural products in crop protection. Bioorganic & Medicinal Chemistry, 17 (2): 4022-4034.
Davidson, W.M., 1929. Insecticidal tests with oils and alkaloids of larkspur (Delphinium consolida) and stavesacre (Delphinium staphisagria). Journal of Economic Entomology, 22 (1): 226-234.
Dev, S. & O. Koul, 1997. Insecticides of Natural Origin. Harwood Academic Publishers, The Netherlands, 222 pp.
Er, M.K., A. Gökçe & M.E. Whalon, 2009. Contact and ingestion toxicities of plant extracts to Thaumetopoea solitaria Frey. (Lepidoptera:Thaumetopoeidae). Journal of Pest Science, 82 (1): 95-99.
Ferry, N., M. Edwards, J. Gatehouse & M. Gatehouse, 2004. Plant-insect interactions: molecular approaches to insect resistance. Current Opinion Biotechnology, 15 (2): 155–161.
Gonzalez-Coloma, A., A. Guadano, C. Gutierrez, R. Cabrera, E. Pena, G. Fuente, M. Reina, 1999. Antifeedant Delphinium diterpenoid alkaloids structure-activity relationships. Journal of Agricultural and Food Chemistry, 46: 286-290.
Gökçe, A., L.L. Stelinski & M.E. Whalon, 2005. Behavioral and electrophysiological responses of leafroller moths to selected plant extracts, Environmental Entomology 34 (6): 1426-1432.
Gökçe, A., R. Isaacs & M.E. Whalon, 2006. Behavioural response of Colorado potato beetle (Leptinotarsa decemlineata) larvae to selected plant extracts, Pest Management Science, 62: 1052–1057.
Gökçe, A., M.E. Whalon, H. Cam, Y. Yanar, İ. Demirtas & N. Gören, 2007. Contact and residual toxicities of 30 plant extracts to Colorado potato beetle larvae. Archives of Phytopathology and Plant Protection, 40 (6): 441–450.
Gökçe, A., L.L. Stelinski, M.E. Whalon & L.J. Gut, 2010. Toxicity and antifeedant activity of selected plant extracts against larval obliquebanded leafroller, Choristoneura rosaceana (Harris). The Open Entomology Journal, 4: 18-24.
Gökçe, A., R. Isaacs & M.E. Whalon, 2011. Ovicidal, larvicidal and anti-ovipositional activities of Bifora radians and other plant extracts on the grape berry moth Paralobesia viteana (Clemens). Journal of Pest Science, 84 (4): 487-493.
Gökçe, A., R. Isaacs & M.E. Whalon, 2012. Dose–response relationships for the antifeedant effects of Humulus lupulus extracts against larvae and adults of the Colorado potato beetle. Pest Management Science, 68: 476–481.
Harborne, J.B., 2001. Twenty-five years of chemical ecology. Natural Product Reports, 18 (4): 361-379.
Isman, M.B., 2002. Insect antifeedants. Pesticide Outlook 13: 152–157.
Isman, M.B., 2006. Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annual Review of Entomology, 51: 45-66.
Isman, M.B., 2008. Perspective botanical insecticides: for richer, for poorer. Pest Management Science, 64: 8-11.
Jannet, H.B., F. H-Skhiri, Z. Mighri, M.S.J. Simmonds & W.M. Blaney, 2001. Antifeedant activity of plant extracts and of new natural diglyceride compounds isolated from Ajuga pseudoiva leaves against Spodoptera littoralis. Industrial Crop and Products, 14: 213-222.
Kleeberg, H., 1992. “The NeemAzal-Conception: Test of Systemic Activity, 5-16”. In: Practice Oriented Results on Use and Production of Neem-Ingredients (Ed. H., Kleeberg). Druck & Graphic, Giessen, Germany, 127 pp.
Koul, O., 2005. Insect Antifeedants. CRC Press, Florida, 1005pp.
Leora Software, 1994. Polo-PC a user’s guide to Probit or Logit analysis, 1119 Shattuck Avenue, Berkeley, CA, 94707.
Mckenzie, J.D. & R. Goldman, 2005. The Student Guide to MINITAB Release 14 Manual. Pearson Education, Boston, MA.
Parakash, A. & J. Rao, 1996. Botanical Pesticides in Agriculture. CRC pres. LewisPublishers, 443 pp.
Pavela, R., 2004. Insecticidal activity of certain medicinal plants. Fitoterapia, 75 (7-8): 745-749.
Pavela, R., 2010. Antifeedant activity of plant extracts on Leptinotarsa decemlineata Say. and Spodoptera littoralis Bois. larvae. Industrial Crops and Products, 32: 213-219.
Pavela, R., 2011. “Natural Products as Allelochemicals in Pest Management,134-148”. In: Natural Products in Pest Management (Ed. N. K. Dubey). Cabi Head Office, Oxfordshire, UK, 312 pp.
Sadek, M.M., 2003. Antifeedant and toxic activity of Adhatoda vasica leaf extract against Spodoptera littoralis (Lep., Noctuidae). Journal of Applied Entomology, 127: 396–404.
Schmidt, G.H., A.I. Ahmed & M. Breuer, 1997. Effect of Melia azedarach extract on larval development and reproduction parameters of Spodoptera littoralis (Boisd.) and Agrotis ipsilon (Hufn.) (Lep. Noctuidae). Anzeiger für Schädlingskunde, 70 (1): 4–12.
Susurluk, H., Z. Calıskan, O. Gürkan, S. Kırmızıgül & N. Gören, 2007. Antifeedant activity of some Tanacetum species and bioassay guided isolation of the secondary metabolites of Tanacetum cadmeum ssp. cadmeum (Compositae). Industrial Crops and Products, 26: 220–228.
Wellsow, J., R.J. Grayer, N.C. Veitch, T. Kokubun, R. Lelli, G.C. Kite & M.S.J. Simmonds, 2006. Insect-antifeedant and antibacterial activity of diterpenoids from species of Plectranthus. Phytochemistry, 67: 1818-1825.
Zapata, N., F. Budia, E. Vinuela & P. Medina, 2009. Antifeedant and growth inhibitory effects of extracts and dirimanes of Driyms winteri stem bark against Spodoptera littoralis (Lep., Noctuidae). Industrial Crops and Products, 30: 119-125.
Zar, J.H., 1996. Biostatistical Analysis. 3rd Ed., Prentice Hall Inc. New Jersey, USA.