Fatma Dolunay ERDOĞUŞ, Durmuş ERDURMUŞ, Mürşide YAĞCI, Harun BAYRAKTAR

Bazı Metarhizium anisopliae (Metschn.) Sorokin, 1883 (Hypocreales: Clavicipitaceae) izolatlarının laboratuvar koşullarında kök-ur nematodları üzerine etkileri

Bu çalışmanın amacı, 2019 yılında Sakarya İli yayla ve meralarından alınan toprak örneklerinden elde edilen entomopatojen fungusların izolasyonunun, teşhisinin yapılması ve Metarhizium anisopliae (Metschn.) Sorokin, 1883 (Hypocreales: Clavicipitaceae) olarak teşhisi yapılan beş adet izolatın (S43/1, S43/2, S43/3, S42/1, S42/2), Meloidogyne incognita (Kofoid & White, 1919) ve Meloidogyne javanica (Treub, 1885) (Tylenchida: Meloidogynidae) ile mücadelede kullanım olanaklarının araştırılmasıdır. Denemeler 2020 yılında Türkiye Cumhuriyeti Tarım ve Orman Bakanlığı, Zirai Mücadele Merkez Araştırma Enstitüsü Müdürlüğü Nematoloji Laboratuvarı’nda laboratuvar koşullarında yürütülmüştür. Uygulamadan 24, 48 ve 72 saat sonra ölü larva sayımları yapılmış ve ölüm oranları hesaplanmıştır. S43/1 izolatı M. javanica’nın kontrolünde en etkili izolat olmuş, 108 cfu/ml dozunda 24 saat sonunda %98,5 ve 48 saat sonunda ise %100 ölüme neden olmuştur. S42/2 izolatı M. incognita’ya karşı en etkili izolat olmuş 24 saat sonunda %97.1 ve 72 saat sonunda %100 ölüme neden olmuştur.

Anahtar Kelimeler:

Biyolojik savaş, Meloidogyne incognita, Meloidogyne javanica, Metarhizium anisopliae

Effects of some Metarhizium anisopliae (Metschn.) Sorokin, 1883 (Hypocreales: Clavicipitaceae) isolates on root-knot nematodes under laboratory conditions

The aim of this study to isolate and diagnose entomopathogenic fungi obtained from soil samples taken from the plateaus and pastures of Sakarya Province in 2019, and to investigate the use of five isolates (S43/1, S43/2, S43/3, S42/1, S42/2) of the diagnosed as Metarhizium anisopliae (Metschn.) Sorokin, 1883 (Hypocreales: Clavicipitaceae) for control Meloidogyne incognita (Kofoid & White, 1919) and Meloidogyne javanica (Treub, 1885) (Tylenchida: Meloidogynidae). The study was conducted at the Laboratory of Nematology in Plant Protection Central Research Institute (Republic of Turkey Ministry of Agriculture and Forestry) in 2020. The experiments were performed under laboratory conditions. Counts of dead larvae were made 24, 48 and 72 h post application and mortality rates calculated. Isolate S43/1 was the most effective isolate for control M. javanica at 108 cfu/ml causing 98.5% mortality after 24 h and 100% after 48 h. Isolate S42/2 was the most effective against M. incognita at 108 cfu/ml and with 97.1% mortality after 24 h and 100% after 72 h.

Keywords:

Biocontrol, Meloidogyne incognita, Meloidogyne javanica, Metarhizium anisopliae,

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Abbasi, M. W., N. Ahmed, M. J. Zaki & S. S. Shuakat, 2018. Nematicidal effects of Bacillus subtilis and Bacillus pumilus against Meloidogyne incognita infecting pea. Advances in Agricultural Science, 6 (4): 52-59.
Abdollahi, M., 2015. Effect of Metarhizium anisopliae and Trichoderma harzianum on root knot nematode Meloidogyne javanica. Biological Control Pest and Diseases, 3 (1): 67-76.
Abdollahi, M., 2018. Application of Metarhizium anisopliae against Meloidogyne javanica in soil amended with oak debris. World Academy of Science, Engineering and Technology International Journal of Agricultural and Biosystems Engineering,12 (2): 40-46.
Bakr, R. A., M. E. Mahdy & E. M. Mousa, 2014. Biological control of root-knot nematode Meloidogyne incognita by Arthrobotrys oligospora. Egyptian Journal Plant Protection, 9 (1): 1-11.
Bruck, D. J., 2005. Ecology of Metarhizium anisopliae in soilless potting media and the rhizosphere: implications for pest management. Biological Control, 32 (1): 155-163.
Butt, T. M., C. Jackson & N. Magan, 2001. “Fungal Biological Control Agents: Progress, Problem and Potential, 1-9”. In: Fungi as Biocontrol Agents (Eds. T. M. Butt, C. Jackson & N. Magan). CABI International, Wallingford, Oxon, U.K., 390 pp.
Devi, G., 2018. Nematophagous fungi:Metarhizium anisopliae. International Journal of Environment, Agriculture and Biotechnology (IJEAB), 3 (6): 2110-2113.
Dijksterhuis, J., M. Veenhuis, W. Harder & B. Nordbring-Hertz, 1994. Nematophagous fungi: physiological aspects and structure-function relationships. Advances in Microbial Physiology, 36: 111-143.
Driver, F., R. J. Milner & J. W. H. Trueman, 2000. A taxonomic revision of Metarhizium based on a phylogenetic analysis of rDNA sequence data. Mycological Research, 104 (2): 143-150.
Er, M. K., 2013. Gaziantep, Adıyaman ve Kahramanmaraş Antepfıstığı bahçelerinde bulunan entomopatojen fungusların tespiti. Türkiye Biyolojik Mücadele Dergisi, 4 (2): 155-163 (in Turkish with abstract in English).
Ghayedi, S. & M. Abdollahi, 2013. Biocontrol potential of Metarhizium anisopliae (Hypocreales:Clavicipitaceae), isolated from suppressive soils of Boyer-Ahmad region, Iran, against J2s of Heterodera avenae. Journal of Plant Protection Research, 53 (2): 165-171.
Griffin, C. T., R. Chaerani, D. Fallon, A. P. Reid & M. J. Downes, 2000. Occurrence and distribution of the entomopathogenic nematodes Steinernema spp. and Heterorhabditis indica. Journal of Helminthology, 74 (2): 143-150.
Gürlek, S. A., F. S. Milletli Sezgin & E. Sevim, 2018. Isolation and characterization of Beauveria and Metarhizium spp. from walnut fields and their pathogenicity against the codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae). Egyptian Journal of Biological Pest Control, 28 (50): 1-6.
Güven Ö., D. Çayır, R. Baydar & İ. Karaca, 2015. Entomopatojen fungus Beauveria bassiana (Bals.) Vull. izolatlarının patates böceği [Leptinotarsa decemlineata Say. (Coleoptera: Chrysomelidae)] üzerindeki etkisi. Türkiye Biyolojik Mücadele Dergisi, 6 (2): 105-114 (in Turkish with abstract in English).
Hoe, P. K., C. F. J. Bong, K. Jugah & A. Rajan, 2009. Evaluation of Metarhizium anisopliae var. anisopliae (Deuteromycotina: Hyphomycete) isolates and their effects on subterranean termite Coptotermes curvignathus (Isoptera: Rhinotermitidae). American Journal of Agricultural and Biological Sciences, 4 (4): 289-297.
Hsiao, Y. M. & J. L. Ko, 2001. Determination of destruxins, cyclic peptide toxins produced by different strains of Metarhizium anisopliae and their mutants induced by ethyl methane sul-fonate and ultraviolet using HPLC method. Toxicon, 39 (6): 837-841.
Humber, R. A., 1997. “Fungi: Identification, 153-185”. In: Manual of Techniques in Insect Pathology (Ed. L. A. Lacey). Academic Press, San Diego, Chapter V-3, 409 pp.
Jahanbazian, L., M. Abdollahi & M. Hussienvand, 2014. Inhibitory effect of Metarhizium anisopliae against Meloidogyne incognita, the causal agent of root knot of tomato, under laboratory condition. National Conference of Modern Topic in Agriculture,12 (2): 40-46.
Kaya, H. K. & S. P. Stock, 1997. “Techniques in Insect Pathology, 281-324”. In: Manual of Techniques in Insect Pathology (Ed. L. A. Lacey). Academic Press, San Diego, Chapter V-3, 409 pp.
Kershaw, M. J., E. R. Moorhouse, R. Bateman, S. E. Reynolds & A. K. Charnley, 1999. The role of destruxins in the pathogenicity of Metarhizium anisopliae for three species of insect. Journal of Invertebrate Pathology, 74 (3): 213-223.
Keskin Y., S. Karabörklü & N. Altın, 2019. Bazı yerel entomopatojen fungusların toprak koşullarındaki etkinliklerinin Tenebrio molitor L. (Col.: Tenebrionidae) larvaları kullanılarak araştırılması. Türkiye Teknoloji ve Uygulamalı Bilimler Dergisi, 2 (1): 26-31 (in Turkish with abstract in English).
Kumar, S., G. Stecher & K. Tamura, 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33 (7): 1870-1874.
Leger, St. R., 2008. Studies on adaptations of Metarhizium anisopliae to life in the soil. Journal of Invertebrate Pathology, 98 (3): 271-276.
Liu, B. L., T. M. Rou, Y. K. Rao & Y. M. Tzeng, 2007. Effect of pH and aeration rate on the production of Destruxins A and B from Metarhizium anisopliae. International Journal of Applied Science and Engineering, 5 (1): 17-26.
Mokhtari, S., N. Sahebani & H. R. Etebarian, 2009. Study on biological control and systemic induction of peroxidase enzyme activity in tomato plant infected with root-knot nematode (Meloidogyne javanica) by Pseudomonas fluorescens CHA0 antagonist. Journal of Agriculture, 11 (1): 151-161.
Mracek, Z. & S. Becvar, 2000. Insect aggregations and entomopathogenic nematode occurrence. Nematology, 2 (3): 297-301.
Richards, M. G. & P. B. Rodgers, 1990. Commercial development of insect biocontrol agents. The exploitation of micro -organisms in applied biology. Aspects of Applied Biology, 318 (1189): 357-373.
Roberts, D. W., 1966. Toxins from the entomogenous fungus Metarhizium anisopliae. 1. Production in submerged and surface cultures, and in inorganic and organic nitrogen media. Journal of Invertebrate Pathology, 8 (2): 212-221.
Saruhan, I., I. C. Erper Tuncer & I. Akca, 2015. Efficiency of some entomopathogenic fungi as biocontrol agents against Aphis fabae Scopoli (Hemiptera:Aphididae). Pakistan Journal of Agricultural Sciences, 52 (2): 273-278.
Sevim, A., M. Höfte & Z. Demirbağ, 2012. Genetic variability of Beauveria bassiana and Metarhizium anisopliae var. anisopliae isolates obtained from the Eastern Black Sea Region of Turkey. Turkish Journal of Biology, 36 (3): 255-265.
Siddiqui, Z. A. & I. Mahmood, 1996. Biological control of plant parasitic nematodes by fungi: a review. Bioresource Technology, 58 (3): 229-239.
Sun, M. H., L. Gao, Y. X. Shi, B. J. Li & X. Z. Liu, 2006. Fungi and actinomycetes associated with Meloidogyne spp. eggs and females in China and their biocontrol potential. Journal of Invertebrate Pathology, 93 (1): 22-28.
Tribhuvaneshwar, S. M. K. & S. Bhargava, 2008. Efficacy of green muscardine fungi, Metarhizium anisopliae against reniform nematode, Rotylenchulus reniformis on tomato. Indian Journal of Nematology, 38 (2): 242-244.
Tuncer, C., R. Kushiyev, J. Liu & İ. Akça, 2018. Metarhizium anisopliae ve Beauveria bassiana izolat ve preparatlarının Curculio nucum’a karşı etkinlikleri. Anadolu Tarım Bilimleri Dergisi, 33 (2):116-123 (in Turkish with abstract in English).
Viaene, N. M. & G.S. Abawi, 2000. Hirsutella rhossiliensis and Verticillium hlamydosporium as biocontrol agents of the root-knot nematode Meloidogyne hapla on Lettuce. Journal of Nematology, 32 (1): 85-100.
White, T. J., T. Bruns, S. Lee & J. Taylor, 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: A Guide to Methods and Applications, 18 (1): 315-322.
Woodring, J. L. & H. K. Kaya, 1988. Steinernematid and Heterorhabditid Nematodes: A Handbook of Biology and Techniques. Arkansas Agricultural Experiment Station, Fayetteville, 30 pp.
Youssef, M. M. A., W. M. A. Nagdi & D. E. M. Lotfy, 2020. Evaluation of the fungal activity of Beauveria bassiana, Metarhizium anisopliae and Paecilomyces lilacinus as biocontrol agents against root-knot nematode, Meloidogyne incognita on cowpea. Bulletin of the National Research Centre, 44 (112): 1-11.
Zimmermann, G., 1986. The “Galleria bait method‟ for detection of entomopathogenic fungi in soil. Journal Applied Entomology, 102 (1-5): 213-215.