Meltem OZGUNER, Ismet Faruk OZGUNER, Namik DELIBAS

Tek Taraflı İnmemiş Testis Modelinde hcg Tedavisi Sonrası Biyokimyasal ve Histopatolojik Değişiklikler

Amaç: Prepubertal sıçan kullanılarak oluşturulan unilateral inmemiş testis modelinde Human Koriyonik Gonadotropin (hCG) tedavisinin etkilerinin serum testosteron ve antisperm antikor (ASA) düzeyleri saptanarak ve stereolojik metodlar kullanılarak değerlendirilmesi. Yöntem ve Gereçler: Toplam 40 adet prepubertal (30 günlük) erkek Spraque-Dawley sıçan Sham, Sham+ hCG tedavisi, İnmemiş testis ve İnmemiş testis+ hCG tedavisi gruplarına ayrıldı. Sıçanlar 30 günlük inmemiş testis ve 30 günlük hCG tedavisi sonrasında sakrifiye edildi. Serum ASA ve testosteron düzeylerini saptamak için kan örnekleri alındı. Testis dokuları rutin histopatolojik işlemlere alındı. Seminifer tübül (parankim) ve interstisiyel alan (stroma) (P/S) oranları stereolojik yöntemler kullanılarak hesaplandı. Bulgular: Testosteron düzeyleri Sham grubunda 689.08 ± 96.08 ng/dl , Sham + hCG grubunda 1062. 32 ± 78.5 ng/dl , İnmemiş testis grubunda 176.09 ± 23.35 ng/dl ve İnmemiş testis+ hCG grubunda 328.75 ± 43.49 ng/dl ölçüldü. ASA düzeyleri Sham grubunda 2.94 ± 0.56 U/ml , Sham + hCG grubunda 5.45 ± 1.58 U/ml, İnmemiş testis grubunda 20.87 ± 4.76 U/ml ve İnmemiş testis+ hCG grubunda 22.12 ± 6.99 U/ml ölçüldü. Testosteron ve ASA düzeyleri açısından Sham grupları ile İnmemiş testis grupları arasında fark saptandı. Parankim/stroma (P/S) oranları karşılaştırıldığında Sham grupları ile İnmemiş testis grupları arasında istatistiksel anlamlı fark saptandı (p<0.05). Ayrıca, P/S oranları açısından değerlendirildiğinde İnmemiş testis ve İnmemiş testis+ hCG grubunun arasında da istatistiksel fark saptandı. Sonuç: Çalışmada hCG tedavisinin P/S oranını parankim lehine çevirdiği bulunmuştur. İnmemiş testis gruplarında ASA düzeylerinin daha yüksek bulunması, çok işleme tabi tutulan testislerde ASA üretiminin artabileceğini düşündürmüştür. hCG tedavisi inmemiş testis grubunda testosteron düzeyini arttırmasına rağmen ASA düzeyini azaltamamıştır. Bulgularımız sonucunda inmemiş testis grubunda hormonal tedavinin morfolojik değişiklikleri ve testosteron düzeylerini etkilediği ancak artmış ASA düzeyini indiremediği saptanmıştır, bu konuya açıklık getirmek için planlanan deneysel çalışmalar unilateral inmemiş testiste karşı testisi de etkileyen patolojik mekanizmaların açıklanmasında yol gösterici olacaktır.

Anahtar Kelimeler:

ASA, hCG, stereoloji, testosteron, inmemiş testis

BIOCHEMICAL AND HISTOPATHOLOGICAL ALTERATIONS IN A UNILATERAL CRYPTORCHIDISM MODEL AFTER hCG TREATMENT

Purpose: To determine the effects of Human Chorionic Gonadotropin (hCG) treatment in unilateral undescended testis model by determining serum testosterone and antisperm antibody (ASA) levels and using stereological methods in prepubertal rats.Methods: A total of 40 prepubertal (30-day old) male Sprague-Dawley rats were separated into Sham, Sham+ hCG treatment, Undescended testis and Undescended testis+ hCG treatment groups. The rats were sacrificed following a 30-day undescended testis duration and 30-day hCG treatment duration. Blood samples were obtained to determine the ASA and testosterone levels. The testes were prepared following routine histological procedures. The seminiferous tubule (parenchyma) and insterstitium (stroma) (P/S) ratios were calculated using stereological methods.Results: The testosterone levels were 689.08±96.08 ng/dl in the Sham group, 1062.32±78.5 ng/dl in the Sham+hCG group, 176.09±23.35 ng/dl in the Undescended testis group and 328.75±43.49 ng/dl in the Undescended testis+hCG group. ASA results were 2.94±0.56 U/ml in the Sham group, 5.45±1.58 U/ml in the Sham+hCG group, 20.87±4.76 U/ml in the Undescended testis group, and 22.12±6.99 U/ml in the Undescended testis+hCG group. A statistically significant difference was detected between the Undescended testis and Sham groups. The comparison of the parenchyma/stroma (P/S) ratios revealed statistically significant difference between Sham groups and Undescended testis groups (p<0.05). There was also statistically significant difference in P/S ratios of Undescended testis and Undescended testis+HCG treatment groups (p<0.05).Conclusion: In this study, it was found that hCG changed the P/S ratio in favor of the parenchyma. The higher level of ASA in groups with an undescended testis indicated that ASA production could be higher in testes that had undergone more handling. Although hCG treatment improved testosterone levels, this treatment did not decrease ASA production in unilateral cryptorchidism. Our findings lead to the conclusion that hormonal treatment effects morphological changes and testosterone levels but could not eliminate elevated ASA production. Further experimental studies can be performed in order to elucidate the mechanisms that effect the other testis in unilateral cryptorchidism

Keywords:

ASA, hCG, stereology, testosterone, undescended testis,

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