Sibel SALMAN YORULMAZ, Reccep AY, Fatma AYDINLI

Predator akar Neoseiulus californicus (McGregor) (Acari: Phytoseiidae)'un dört farklı popülasyonunun spirodiclofen, hexythiazox, etoxazole karşı direnç düzeyleri ve direnç mekanizmalarının belirlenmesi

Bu çalışmada, Isparta ili elma bahçelerinden 2013 yılında toplanan Neoseiuluscali fornicus (McGregor) (Acari: Phytoseiidae) popülasyonlarının spirodiclofen,hexythiazox ve etoxazole karşı duyarlılık düzeyleri biyoassay yöntemlerle belirlenmiştir. N.cali fornicus popülasyonlarının LC50 değeri ilaçlama kulesi kullanılarak yaprak disk metoduile bulunmuştur. 2013 yılında elma bahçelerinden toplanan N. cali fornicus'un Gönen,Esinyurt, Gelendost ve Ağılköy beldelerinden toplanan popülasyonlarında spirodiclofen'ekarşı sırasıyla 6.36, 7.52, 5.12 ve 6.57 kat; hexythiazox‟a karşı 7.75, 8.02, 5.23 ve 8.06 katve etoxazole karşı 11.47, 13.31, 6.59 ve 11.75 kat direnç belirlenmiştir. Ayrıca N.cali fornicus popülasyonlarında PBO, IBP ve DEM sinerjistlerinin akarisiler ile sinerjistiketkileri incelenmiştir. PBO, IBP ve DEM sinerjistik etki sonuçları Gönen popülasyonu için˂1-1.37 kat, 1.02-1.35 kat ve ˂1 kat; Esinyurt popülasyonu için ˂1-1.26 kat, 1.23-1.33 katve ˂1 kat; Gelendost popülasyonu için ˂1, ˂1-1.01 ve ˂1 kat; Ağılköy popülasyonu için˂1-1.05 kat, 1.02-1.72 kat ve ˂1 kat değerleri arasında belirlenmiştir. N. cali fornicus popülasyonlarında glutathion S-transferaz (GST) ve monooksigenaz (P450) enzimlerikinetik yöntemle, esteraz enzimi elektroforez ve kinetik yöntemlerle belirlenmiştir. Esteraz,glutathion S-transferaz (GST) ve monooksigenaz (P450) enzim aktiviteleri sırasıyla 8.98- 11.05, 2.05-2.62 ve 0.0239-0.0426 mOD min-1 mg -1 protein olarak bulunmuştur.

Resistance levels and resistance mechanisms of four different populations of the predatory mite Neoseiulus californicus (McGregor) (Acari:Phytoseiidae) against spirodiclofen, hexythiazox and etoxazole

In this study, the sensitivity levels of Neoseiulus cali fornicus (McGregor) (Acari: Phytoseiidae) populations collected from apple orchards in Isparta province in 2013to spirodiclofen, hexythiazox and etoxazole were determined by means of bioassay. TheLC50 values of N. cali fornicus populations were determined with the leaf disk method and aspray tower. In the populations of N. cali fornicus collected from apple orchards in Gönen,Esinyurt, Gelendost and Ağılköy towns, 6.36-, 7.52-, 5.12- and 6.57-fold resistance tospirodiclofen was found, respectively; 7.75-, 8.02-, 5.23- and 8.06-fold resistance tohexythiazox was found, respectively; and 11.47-, 13.31-, 6.59- and 11.75-fold resistance toetoxazole was found, respectively. Furthermore, the effects of the synergists PBO, IBP andDEM were studied in N. cali fornicus populations. The results for the synergistic effects ofPBO, IBP and DEM were ˂1-1.37, 1.02-1.35 and ˂1-fold, respectively, for the Gönenpopulation; ˂1-1.26, 1.23-1.33 and ˂1 fold, respectively, for the Esinyurt population; ˂1,˂1-1.01 and ˂1fold, respectively, for the Gelendost population; and ˂1-1.05, 1.02-1.72 and ˂1 fold for the Ağılköy population. In the populations of N. cali fornicus, glutathioın S- transferase (GST) and monooxygenase (P450) enzymes were identified with the kineticmethod and the esterase enzyme was identified through electrophoresis and the kineticmethod. The enzyme activities of esterase, glutathione S-transferase (GST) andmonooxygenase (P450) were 8.98-11.05, 2.05-2.62 and 0.0239-0.0426 mOD min-1 mg -1 protein, respectively

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