Nispi nemin yumurta parazitoidi Trichogramma pintoi Voegele (Hymenoptera: Trichogrammatidae)'nin farklı biyolojik dönemlerine etkisi

Özet: Bu çalışmada yumurta parazitoidi Trichogramma pintoi Vogelé (Hymenoptera:Trichogrammatidae) 25±1ºC sıcaklık % 60-70 orantılı nem ve 16: 8 aydınlık: karanlıkkoşulların sağlandığı iklim odasında yetiştirilmiştir. Denemelerde parazitoidin ergindönemi hariç diğer gelişme dönemleri farklı farklı nispi nem oranlarına (% 20-30, % 50-60, % 70-80) tabi tutulmuş ve parazitoitin gelişimi incelenmiştir. Parazitoid yumurta ve larvadönemindeyken, farklı nem koşulları parazitoidin gelişimini etkilememiştir. Ancakparazitoid pre-pupa ve pupa dönemindeyken nispi nemin düşürülmesi parazitoid gelişiminiolumsuz etkilemiştir. Parazitoid pre-pupa dönemindeyken nispi nemin % 20-30‟adüşürülmesi çıkış oranında kontrole göre % 9.68‟lik azalmaya neden olmuştur. Benzerşekilde parazitoid pupa dönemindeyken de nispi nemin % 20-30 ile % 50-60‟a düşürülmesi çıkış oranında sırası ile % 22.82 ve % 7.62‟lik bir azalmaya neden olmuştur. Diğer taraftan aynı nispi nem oranları gelişme süresinde sırası ile % 17.74 ve % 2.60‟lık bir artışa nedenolmuştur. Nispi nem parazitoidin farklı gelişme dönemleri için son derece önemlidir. Bu çalışmanın sonuçları, laboratuvar koşullarında uygun nem değerinin sağlanması parazitoidT.pintoi‟nin kitle üretim tekniğinin iyileştirilmesine katkı sağlayabilecektir.

Effect of relative humidity of the different developmental stages of the egg parasitoid Trichogramma pintoi Voegele´ (Hymenoptera: Trichogrammatidae)

Abstract: In this study, the egg parasitoid Trichogramma pintoi Vogelé (Hymenoptera:Trichogrammatidae) was reared in a controlled climate room at 25±1 ºC, 60-70% RH and16 L:8D. During the experiment, different stages, except for adults, were subjected tovarious relative humidity levels (20-30%, 50-60%, 70-80%) and its development wasexamined. During the egg and larval stages, the various relative humidities did not have anyeffect on the parasitoid‟s development. However, its development was adversely affectedby reduced relative humidity levels during the pre-pupal and pupal stages. Decreasinghumidity levels to 20-30% at the pre-pupal stage resulted in a 9.68% reduction in theemergence ratio of adults compare with the control. Similarly, the percentage of adultemergence from the pupal stage was decreased by 22.82% and 7.62% by lowering relativehumidity levels to 20-30% and 50-60%, respectively. On the other hand, the same order ofhumidity levels prolonged pupal development time by 17.74% and 2.60 %, respectively. Relative humidity is therefore very important for the different developmental stages of theparasitoid. The results of this study showed that maintaining suitable humidity levels underlaboratory conditions can help to improve mass-rearing techniques for T. pintoi.

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Akbari F., A. Askarianzadeh, A. A. Zamani & M.H. Hosseinpour 2012. Biological characteristics of three Trichogramma species on the eggs of diamondback moth (Plutella xylostella L.). Archives of Phytopathology and Plant Protection, 45(19), 2364- 2368. Bulut H. & N. Kılınçer 1989. Ankara ilinde meyve ağaçlarında zarar yapan önemli lepidopterlerin yumurta parazitlerinden Trıchogramma türleri (Hym: Trichogrammatidae) ve bunların yayılıĢı üzerinde araĢtırmalar. Bitki Koruma Bülteni, 29 (1-2):19-46. Chapman R.F. 1973. The Insect: Structure and Function. The English Universities Press LTd London, Pp 819. Cônsoli F.L., J.R.P. Parra & S.A. Hassan 1998. Side-effects of insecticides used in tomato fields on the egg parasitoid Trichogramma pretiosum Riley (Hym., Trichogrammatidae), a natural enemy of Tuta absoluta (Meyrick) (Lep., Gelechiidae). Journal of Applied. Entomology, 122: 4347. Croft P & M.J.W. Copland 1994. The ınfluence of humidity on emergence in Dacnusa sibirica Telenga (Hymenoptera:Braconidae) Biocontrol scıence and Technology, 4:347- 351. Ebrahimi E. 2004. Investigation and Identification of species of Trichogramma Westwood in Iran. In: Azema M. & A. Mirabzadeh, editors. Issues on Di fferent Aspects of Applying Natural Enemies for Biological Control of Insect Pests, Nashre Markaz, Tehran, Iran, Pp. 171. Farghaly H.T.H. & Z.A. Ragab 1984. Relationships between relatıve humidity and adult biology of Bracon hebetor Say. Bulletin Societe Entomologique, 65, 131-135. Fathipour Y. & H. D. Moghanloo 2003. Comparative biology of Trichogramma pintoi Voegele wasps reared on two of laboratory hosts. Iranian Journal of Agricultural Sciences, 34 (4): 881-888. Gross H.R. 1988. Effect of temperature, relative humidity, and free water on the number and normalcy of Trichogramma pretiosum Riley (Hymenoptera, Trichogrammatidae) emerging from eggs of Heliothis zea (Boddie) (Lepidoptera: Noctuidae). Environmental Entomology, 17: 470-475. Keita F.B. 1988. Formation of laboratory populations of Trichogramma maidis (Pintureau - Voegele) and Trichogramma pintoi (Pintureau - Voegele) Hymenoptera - Trichogrammatidae. Rasteniev'dni Nauki, 25(9):115-120. Knutson A. 1998. The Trichogramma Manual. Agricultural Communications, Agricultural Extension Service. A&M University System College Station, TX, USA. Pp: 37. Koçlu T. & Y. Karsavuran 1998. Manisa ilinde Helicoverpa armigera (Huebner) (Lepidoptera: Noctuidae)‟nın yumurtalarının parazitoidleri ve doğal etkinlikleri. Türkiye Entomoloji Dergisi, 22(4): 269-283. Patil G.M. & R. Govindan 1986. Investigation on certain factors governing the biotic potential of the uzi-fly, Exorista sorbillans (Weidemann) (Diptera, Tachinidae) Indian Journal of Sericulture, 25: 45-53. Shojai M., H. Ostovan, A.R. Khodaman, M. Hosseini, M. Daniali, M. Seddighfar, A. A. Nasrollahi, Y. Labbafi, F. Ghavam & S. Honarbakhsh 1998. An investigation on beneficial species of Trichogramma spp. (Hym., Trichogrammatidae), active in apple orchards, and providing optimum conditions for mass production in laboratory cultures. Journal of Agricultural Sciences, Islamic Azad University, 16: 539. Smith S.M. 1996. Biological control with Trichogramma: advances, successes, and potential of their use. Annual Review of Entomology, 41: 375406. Sorokina A.P. 1999. Trophic links of species of the genus Trichogramma West. (Hym. Trichogrammatidae) of the world fauna. Entomological Review,79: 125132. Suh C.P.C., D.B. Orr & J.W.V. Duyn 2000. Effect of insecticides on Trichogramma exigum (Hymenoptera: Trichogrammatidae) preimaginal development and adult survival. Journal of Economic Entomology, 93: 577583. Wang F.C. & S.Y. Zhang 1988. Studies on Trichogramma pintoi (Hym.: Trichogrammatidae): its deuterotokous reproduction, artificial propagation and field releases. Chinese journal of Biological Control, 4 (4):149-151. Wang F.C. & S.Y. Zhang 1991. ,Trichogramma pintoi (Hym.: Trichogrammatidae): deuterotoky, laboratory multiplication and field releases. Trichogramma pintoi (Hym.: Trichogrammatidae): deuterotoky, laboratory multiplication and field releases. Colloques de l' INRA, 56: 155-157. Xequi, G. 1988. Bionomics of Trichogramma ostriniae Pang et Chen. Les Colloques de l INRA, 43: 191-195. Yadav R.P. & J.P. Chaudhary 1986. Longevity and development of the parasitoid Cheiloneurus pyrillae (Hymenoptera: Encyrtidae) at different combinations of temperature and relative humidity. Entomophaga, 31: 107-111.