Bitki gelişimini teşvik eden bazı bakterilerin marulun gelişimi ve Bakteriyel yaprak lekesi hastalığı üzerine etkileri

Dünyanın farklı bölgelerinde marul yaprak lekesi hastalığına neden olan Xanthomonas axonopodis pv. vitians, ülkemizde ilk kez 2000 yılında görülmüştür. Kimyasal pestisitler kullanılarak hastalığın kontrolü zordur. Ülkemizde, marulda görülen bakteriyel hastalıkların mücadelesi için ruhsatlandırılmış herhangi bir bakterisit de bulunmamaktadır. Bu çalışmada, MIS tanı sonuçlarına göre Pantoea (33 izolat), Bacillus (6 izolat) ve Paenibacillus (1 izolat) cinslerine mensup 40 aday bitki gelişimini teşvik edici rizobakteri (PGPR) ve bitki gelişimini teşvik edici endofitik (PGPE) bakterinin, hastalık şiddeti ve marulun gelişimi üzerine etkileri araştırılmıştır. Tüm izolatlar MIS (Mikrobiyal Tanı Sistemi) ve Biolog sistemleri ile tanılanmış, fosfatı çözme ve azotu fikse etme yetenekleri test edilmiştir. İzolatların tamamı azotsuz (N-free) besiyerinde gelişim göstermiş ve azotu fikse etme yetenekleri pozitif olarak değerlendirilmiştir. Yine izolatların cözülemez formda bulunan fosfatı 40.95-227.20 µg ml-1 arasında değişen değerlerde çözebildiği tespit edilmiştir. Bu izolatlar arasından fosfatı çözme yeteneği en fazla olan 11 tanesi ve bunların 4

Anahtar Kelimeler:

Xanthomonas axonopodis pv. vitians, PGPR, PGPE, marul

Effects of some plant growth promoting bacteria on growth of lettuce and Bacterial leaf spot disease

Bacterial leaf spot of lettuce caused by Xanthomonas axonopodis pv. vitians (Brown) Dye. is present in several parts of the world and was observed for the first time in Turkey in 2000. Management of the pathogen with chemical pesticides provides limited control. Presently, no bactericides are registered in Turkey for controlling bacterial diseases on lettuce. In this study, according to MIS (Microbial Identification System) identification result, forty candidate plant growth promoting rhizobacteria (PGPR) and plant growth promoting endophytic bacteria (PGPE) from the genera Pantoea (33 strains), Bacillus (6 strains) and Paenibacillus (1 strain) were tested for their ability to control the pathogen and on growth of lettuce. All of the bacterial strains were identified by MIS and Biolog System. All isolates were able to grow in N-free media indicating their abilities to produce nitrogen. This isolates were able to solubilize insoluble phosphate which ranged from 40.95-227.20 µg ml-1 . The eleven phosphate solubilizing strains and their four different combinations were selected for greenhouse experiment. Consequently, our results indicated that some of tested strains and their some combinations had a positive effect on lettuce growth and ability to suppress disease growth.

Keywords:

Xanthomonas axonopodis pv. vitians, PGPR, PGPE, lettuce,

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