Aims: The aim of this study is to determine the level of information regarding radiation safety and the side effects related to the use of fluoroscopy among a group of teaching assistants, research assistants, medical students, nurses, technicians and caretakers working at Trakya University Health Center for Medical Research and Practice.Methods: A descriptive, cross-sectional survey study was conducted from December 2014 to April 2015 at Trakya University Health Center for Medical Research and Practice. The questionnaire consisted of 24 questions and was applied to 53 people. Responses regarding radiation safety and the side effects of fluoroscopy were analyzed in Statistical Package for the Social Sciences (SPSS) 20.0 using descriptive statistics including frequencies and percentages.Results: 98.08% of the participants responded “yes” to the question ”Do you think fluoroscopy is hazardous to health?”. On the other hand, the results showed that only 32.69% of the participants wore lead apron and 32.77% used thyroid shields in every operation. 88.46% of the participants reported that they would like to attend a training regarding fluoroscopy and radiation safety.Conclusion: The results indicated that the operating room staff of Trakya University Health Center for Medical Research and Practice does not possess adequate knowledge regarding radiation safety