Transmission of Microsporidium sp. between different generations of Crepidopdera aurata (Coleoptera: Chrysomelidae)

Microsporidia are mostly very small, intracellular, spore-forming pathogens classified as Protista and reported in every majorgroup of animals. Insects are one of the most widely distributed hosts for microsporidia. Many entomopathogenic microsporidia causechronic infections in insects and their effects appear as reduced fertility and pupal weight, reduced fecundity and shortened longevity.Crepidopdera aurata (Coleoptera; Chrysomelidae) is one of the most destructive pests in poplar nurseries. The host-parasite relation inmicrosporidium infections is of great importance to understand the possibilities to use microsporidia in the host’s biological control. Inthe present study, the transmission of the microsporidian pathogen Microsporidium sp. was studied between different generations (inspring and summer) of C. aurata in 2015, 2016, 2018, and 2019. The results showed that there is considerable difference in the infectionrates of the offspring and parental beetles. The results confirm that the infection rates in the offspring beetles (new generation in thesame year) are higher than that in parental beetles (first generation in the same year) in the 2 populations for all investigated years. Onthe other hand, statistical analysis showed that Microsporidium sp. does not favour either of the sexes in C. aurata populations. Possibletransmission of the microsporidium between different generations is also discussed according to the literature.

PDF

___

Agnew P, Becnel JJ, Ebert D, Michalakis Y (2003). Symbiosis of microsporidia and insects. In: Bourtzis K, Millar TA, editors. Insect symbiosis. Boca Raton, FL, USA: CRC Press, pp. 145-163.
Becnel JJ, Andreadis TG (1999). Microsporidia in insects. In: Wittner M, Weiss LM, editors. The Microsporidia and Microsporidiosis. Washington DC, USA: ASM Press, pp. 447-501.
Brooks WM (1988). Entomogeneous protozoa. In: Ignoffo CM (editor). Handbook of Natural Pesticides. Boca Raton, FL, USA: CRC Press, pp. 1-149.
Hausmann K, Hülsmann N, Radek N (2003). Protistology. 3rd completely revised edition. E. Schweizerbart’sche Verlagsbuchhandlung (Nägele u. Obermiller). Berlin, Stuttgart, Germany: ISBN 3–510–65208–8, hardback, pp 379.
Lipa JJ, Hokkanen HMT (1992). Nosema meligethi I. & R. (Microsporida) in populations of Meligethes spp. in Europe. Biocontrol Science and Technology 2: 119-125.
Malone LA, Wigney PJ (1980). The distribution of Nosema carpocapsae, a protozoan pathogen of the codling moth, Cydia pomonella (Lepidoptera: Tortricidae), in New Zealand. New Zealand Entomologist 7: 151-153.
Rosicky B (1951). Nosematosis of Otiorrhynchus ligustici, II. The influence of the parasitation by Nosema otiorrhynchi W. on the susceptibility of the beetles to insecticides. Vestnik ceskoslovenskogo Zoologickoj Spolecnosti 15: 219-230.
Sprague V, Becnel JJ, Hazard EI (1992). Taxonomy of phylum microspora. Critical Reviews in Microbiology 18: 285-395.
Urban J (2011). Occurrence, bionomics and harmfulness of Crepidodera aurata (Marsh.) (Coleoptera, Alticidae). Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 59: 263-278.
Weiser J (1961). A new microsporidian from the bark beetle Pityokteines curvidens Germ. (Col., Scolyt.) in Czechoslovakia. Journal of Insect Pathology 3: 324-329.
Yaman M (2007). Distribution of Nosema meligethi I. & R. (Microsporida) in populations of Meligethes aeneus (Coleoptera: Nitidulidae) in Turkey. Entomological Research 37: 298-301.
Yaman M (2008). First results on the distribution of Nosema chaetocnemae (Microspora) in the populations of Chaetocnema tibialis (Coleoptera, Chrysomelidae) in Turkey. Turkish Journal of Parasitology 32: 94-98.
Yaman M, Ertürk Ö (2016). Isolation, identification and insecticidal effects of entomopathogenic bacteria from the willow flea beetle, Crepidodera aurata (Coleoptera; Chrysomelidae). Progress in Plant Protection 56: 225-229.
Yaman M, Algı G, Güner BG, Ünal S (2015). Distribution and occurrence of microsporidian pathogens of the willow flea beetle, Crepidodera aurata (Coleoptera, Chrysomelidae) in North Turkey. Entomologica Fennica 26: 171-176.
Yaman M, Eroğlu M, Radek R (2016). Occurrence of a microsporidium in the predatory beetle Calosoma sycophanta L. (Coleoptera: Carabidae). Turkish Journal of Agriculture and Forestry 40: 420-424.
Yaman M, Algı G, Radek R (2019a). Morphological, ultrastructural and molecular identification of a new microsporidian pathogen isolated from Crepidodera aurata (Coleoptera, Chrysomelidae). Turkish Journal of Zoology 43: 407-415.
Yaman M, Uzuner S, Güner BG, Ayar Ö, Ertürk Ö et al. (2019b). Distribution of microsporidial infection in the predator beetle, Calosoma sycophanta (Coleoptera:Carabidae)-rearing laboratories in Turkey. Turkish Journal of Agriculture and Forestry 43: 586-592.