The Sicilian rock partridge: latest data on genetic integrity from four different relict areas
The Sicilian rock partridge: latest data on genetic integrity from four different relict areas
Abstract: Sicily (Italy) hosts a “relict”, endemic population of the birds Alectoris graeca whitakeri commonly known as Sicilian Rock Partridge. In the last decades, due to the risk of restocking with other European and Asiatic species for hunting purpose, a study was carried out to investigate the potential risk of hybridisation. The mtDNA control-region and nuclear microsatellites were genotyped. Due to the importance of the species, samples were mainly characterized by feather and stool samples, and rarely by carcasses found in the environment, from year 2011 to 2012. A panel of 7 microsatellite loci was validated. Three multiplexes that allowed the simultaneous amplification of 3 microsatellites, and 2 for other two microsatellites, for a total of 7 markers, were utilized. Results showed the occurrence of hybridization both towards the Middle Eastern species, A. chukar and the Northern European species, A. rufa. A total of 18.5% of the samples were collected from the wild environment showed a high degree of hybridization. This fact, even if linked to a small number of samples, highlights a potential risk of hybridization in 4 Sicilian provinces and underlines the importance of further investigations to understand the entity of the problem.Key words: Alectoris, genetic diversity, microsatellites, Sicily
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- Barbanera F, Guerrini M, Khan AA, Panayides P, Hadjigerou P et al. (2009). Human-mediated introgression of exotic chukar (Alectoris chukar, Galliformes) genes from East Asia into native Mediterranean partridges. Biological Invasions 11: 333-348.
- Barilani M, Sfougaris A, Giannakopoulos A, Mucci N, Tabarroni C (2007). Detecting introgressive hybridisation in rock partridge populations (Alectoris graeca) in Greece through Bayesian admixture analyses of multilocus genotypes. Conservation Genetics 343-354.
- Casas F, Mougeot F, Sanchez-Barbudo I, Davila JA, Vinuela J (2012). Fitness consequences of anthropogenic hybridization in wild red- legged partridge (Alectoris rufa, Phasianidae) populations. Biological Invasions 14: 295-305.
- Del Hoyo J, Collar NJ, Christie DA, Elliott A, Fishpool LDC (2014). HBW and BirdLife International Illustrated Checklist of the Birds of the World. Lynx Edicions BirdLife International, Barcelona, Spain and Cambridge, UK.
- Evanno G, Regnaut S, Goudet J (2005). Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Molecular Ecology 14: 2611-2620.
- Falush D, Stephens M, Pritchard JK (2003). Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies. Genetics 164: 1567-1587.
- Francis RM (2017). Pophelper: an R package and web app to analyse and visualize population structure. Molecular Ecology Resources 17 (1): 27-32. doi:10.1111/1755-0998.12509
- Kalinowski ST, Taper ML, Marshall TC (2007). Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Molecular Ecology 16: 1099-1106.
- Madge S, McGowan P (2002). Pheasants, Partridges and Grouse. Including buttonquails, sandgrouse and allies. Helm Identification Guides, Christopher Helm, London.
- Martínez-Fresno M, Henriques-Gil N, Arana P (2008). Mitochondrial DNA sequence variability in red-legged partridge, Alectoris rufa, Spanish populations and the origins of genetic contamination from A. chukar. Conservation Genetics 9: 1223-1231.
- Monaco A, Carnevali L, Toso S (2010). Linee guida per la gestione del cinghiale (Sus scrofa) nelle aree protette. II edizione. Quad. Cons. Natura, 34, Min. Ambiente – ISPRA.
- Palumbo G, Lo Valvo M (2002). Management Statement for the Sicilian Rock Partridge (Alectoris graeca whitakeri). BirdLife International/European Commission, T-PVS/Inf 18.
- Pritchard JK, Stephens M, Donnelly P (2000). Inference of population structure using multilocus genotype data. Genetics 155: 945–959.
- Randi E (2006). Evolutionary and conservation genetics of the rock partridge Alectoris graeca. Acta Zoologica Sinica 52: 370- 374.
- Randi E, Lucchini V (1998). Organization and Evolution of the Mitochondrial DNA Control Region in the Avian Genus Alectoris. Journal of Molecular Evolution 47: 449-462.
- Randi E, Tabarroni C, Rimondi S, Lucchini V, Sfougaris A (2003). Phylogeography of the Rock Partridge (Alectoris graeca). Molecular Ecology 12: 2201-2214.
- Raymond M, Rousset F (1995). GENEPOP (version 4.0.11): population genetics software for exact tests and ecumenicism. Journal of Heredity 86: 248-249.
- Rondinini C, Battistoni A, Peronace V, Teofili C (compilatori) (2013). Lista Rossa IUCN dei Vertebrati Italiani. Comitato Italiano IUCN e Ministero dell’Ambiente e della Tutela del Territorio e del Mare, Roma.
- Schiebel G (1934). Alectoris graeca whitakeri subsp. nova. Steinhuhn von Sizilien. Falco 30: 2-3.
- Sorace A, Artese C, Antonucci A, Bernoni M, Bonani M et al. (2013). Status and distribution of rock partridge Alectoris graeca in Apennine areas. Avocetta 37 (2): 111-118.
- Tejedor MT, Monteagudo L, Arruga V (2008). Microsatellite markers for the analysis of genetic variability and relatedness in red-legged partridge (Alectoris rufa) farms in Spain. Research in veterinary science doi: 10.1016/j.rvsc.2007.08.004.
- Tejedor MT, Monteagudo LV, Mautner S, Hadjisterkotis E, M Arruga V (2007). Introgression of Alectoris chukar Genes into a Spanish Wild Alectoris rufa Population. Journal of Heredity 98 (2): 179-182. doi: 10.1093/jhered/esm001.