Behzad FATHINIA, Nasrullah RASTEGAR-POUYANI, Eskandar RASTEGAR-POUYANI, Fatemeh TOODEH-DEHGHAN, Mehdi RAJABIZADEH

Molecular systematics of the genus Pseudocerastes (Ophidia: Viperidae) based on the mitochondrial cytochrome b gene

The false horned vipers of the genus Pseudocerastes consist of 3 species; all have been recorded in Iran. These include Pseudocerastes persicus, P. fieldi, and P. urarachnoides. Morphologically, the taxonomic border between P. fieldi and P. persicus is not as clear as that between P. urarachnoides and P. persicus or P. fieldi. Regarding the weak diagnostic characters differentiating P. fieldi from P. persicus and very robust characters separating P. urarachnoides from both, there may arise some uncertainty in the exact taxonomic status of P. urarachnoides and whether it should remain at the current specific level or be elevated to a distinct genus. Based on our sequence data from the mitochondrial cytochrome b gene, the taxonomic status of the 3 false horned vipers is confirmed. Although P. fieldi has equal genetic distance from P. persicus and P. urarachnoides, the spider-tailed viper is more closely related to P. persicus than P. fieldi. There are also some subdivisions among the populations of P. persicus in Iran.

Anahtar Kelimeler:

Cytochrome b, genetic divergence, molecular systematics, Pseudocerastes, Viperidae, Iran

Molecular systematics of the genus Pseudocerastes (Ophidia: Viperidae) based on the mitochondrial cytochrome b gene

Keywords:

Cytochrome b, genetic divergence, molecular systematics, Pseudocerastes, Viperidae, Iran,

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Bostanchi H, Anderson SC, Kami HG, Papenfuss TJ (2006). A new species of Pseudocerastes with elaborate tail ornamentation from western Iran (Squamata: Viperidae). Proc Calif Acad Sci 4: 443– 450.
Fathinia B, Anderson SC, Rastegar-Pouyani N, Jahani H, Mohamadi H (2009). Notes on the natural history of Pseudocerastes urarachnoides (Squamata: Viperidae). Russ J Herpetol 16: 134–138.
Fathinia B, Rastegar-Pouyani N (2010). On the species of Pseudocerastes (Ophidia: Viperidae) in Iran. Russ J Herpetol 17: 275–279.
Felsenstein J (1985). Confidence limits on phylogenies: an approach using the bootstrap. Evol 39: 783–791.
Hall TA (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symp Ser 41: 95–98.
Latifi M (2000). Snakes of Iran. 3rd ed. Tehran, Iran: Department of the Environment Edition (in Farsi).
Leviton AE, Anderson SC, Adler K, Minton SA (1992). Handbook to Middle East Amphibians and Reptiles. Oxford, OH, USA: SSAR.
Librado P, Rozas J (2009). DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25: 1451– 1452.
Phelps T (2010). Old World Vipers: A Natural History of the Azemiopinae and Viperinae. 1st ed. Frankfurt, Germany: Edition Chimaira.
Saitou N, Nei M (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425.
Sambrook J, Fritsch EF, Maniatis T (1989). Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor, NY, USA: Cold Spring Harbor Laboratory Press.
Stümpel N, Joger U (2009). Recent advances in phylogeny and taxonomy of Near and Middle Eastern Vipers – an update. ZooKeys 31: 179–191.
Swofford DL (2001). PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Models), Version 4.0b10. Sunderland, MA, USA: Sinauer Associates.
Tamura K, Stecher G, Peterson D, Filipsky A, Kumar S (2013). MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 30: 2725–2729.
Wüster W, Peppin L, Pook CE, Walker DE (2008). A nesting of vipers: phylogeny and historical biogeography of the Viperidae (Squamata: Serpentes). Mol Phyl Evol 49: 445–459.