Molecular identification of invasive drosophilid Zaprionus tuberculatus Malloch (Diptera: Drosophilidae) in Çanakkale, Turkey

Molecular identification of invasive drosophilid Zaprionus tuberculatus Malloch (Diptera: Drosophilidae) in Çanakkale, Turkey

The vinegar or pomace fly, Zaprionus tuberculatus Malloch, is native to the Afrotropical region but has recently colonizednew areas in Turkey, Italy, Malta, Romania, and Greece. This invasive species was first detected in 2012 in Adana on banana and peachbaits and is considered a potential agricultural pest in fig production in Turkey. However, the European and Mediterranean PlantOrganization reports that no direct association of the fly with fruit damage has been documented so far because specimens weregenerally caught in traps. Specimens of Zaprionus sp. were monitored during surveys for cases of Drosophila suzukii (Matsumura)and Ceratitis capitata (Wiedemann) damaging experimental pomegranate and fig fields in Çanakkale, where we performed the fieldstudy. To better understand the species identification, a partial sequence of the mitochondrial cytochrome oxidase I (COI) gene regionwas used in this study. The barcode primer pairs were used to amplify the partial COI gene. PCR products were visualized and thendirectly sequenced. No insertion or deletion was observed in the 637-bp COI gene region. The average nucleotide frequencies of T, A,C, and G were observed as 39.1%, 29.6%, 15.2%, and 16.1%, respectively. Intraspecific pairwise divergence was low at 0.15%. Nucleotidesequences indicated that there were 2 substitutions, an A→G at 343 and a C→T at 592 positions, respectively. In conclusion, this studypresents the first molecular evidence and presence of Z. tuberculatus in Çanakkale, Turkey, which can help in resolving morphologicambiguities.

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  • Altschul SF, Gish W, Millet W, Myers EW, Lipman DJ (1990). Basic local alignment search tool. Journal of Molecular Biology 15 (3): 403-410.
  • Araripe LO, Klaczko LB, Moreteau B, David JR (2004). Male sterility thresholds in a tropical cosmopolitan drosophilid: Zaprionus indianus. Journal of Thermal Biology 29: 73-80.
  • Commar LS, Galego LGC, Ceron CR, Carareto CMA (2012). Taxonomic and evolutionary analysis of Zaprionus indianus and its colonization of Palearctic and Neotropical regions. Genetics and Molecular Biology 35: 395-406.
  • Joshi NK, Biddinger DJ, Demchak K, Deppen A (2014). First report of Zaprionus indianus (Diptera: Drosophilidae) in commercial fruits and vegetables in Pennsylvania. Journal of Insect Science 14: 1-4.
  • Kremmer L, David J, Borowiec N, Thaon M, Ris N et al. (2017). The African fig fly Zaprionus indianus: a new invasive pest in France? Bulletin of Insectology 70 (1): 57-62.
  • Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018). MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution 35 (6): 1547-1549.
  • Lachaise D, Tsacas L (1983). Breeding-sites in tropical African drosophilids. In: Ashburner M, Carson HL, Thompson JN, (editors). The Genetics and Biology of Drosophila. Vol. 3a. London, UK: Academic Press, pp. 221-332.
  • Lasa R, Tadeo E (2015). Invasive drosophilid pests Drosophila suzukii and Zaprionus indianus (Diptera: Drosophilidae) in Veracruz, Mexico. Florida Entomologist 98: 987-988.
  • Leivadaras J, Leivadara EI, Vontas J (2017). A new insect, drosophila Zaprionus tuberculatus (Diptera: Drosophilidae), affects the figs on Crete. In: The Panhellenic Entomological Congress; Athens, Greece. p. 27.
  • Mutun S, Atay G (2015). Phylogeography of Trigonaspis synaspis (Hymenoptera: Cynipidae) from Anatolia based on mitochondrial and nuclear DNA sequences. European Journal of Entomology 112 (2): 259-269.
  • Patlar B, Koc B, Yilmaz M, Ozsoy ED (2012). First records of Zaprionus tuberculatus (Diptera: Drosophilidae) from the Mediterranean Region, Turkey. Drosophila Information Service 95: 94-96.
  • Raspi A, Grassi A, Benelli G (2014). Zaprionus tuberculatus (Diptera Drosophilidae) first records from the European mainland. Bulletin of Insectology 67 (1): 157-160.
  • Rokas A, Willams BL, King N, Carroll SB (2003). Genomescale approaches to resolving incongruence in molecular phylogenies. Nature 425 (6960): 798-804.
  • Tsacas L, Lachaise D, David JR (1981). Composition and biogeography of the Afrotropical drosophilid fauna. In: Ashburner M, Carson HL, Thompson JN (editors). The Genetics and Biology of Drosophila. Vol. 3a. London, UK. Academic Press, pp. 197- 259.
  • Walsh DB, Bolda MP, Goodhue RE, Dreves AJ, Lee J et al. (2011). Drosophila suzukii (Diptera: Drosophilidae): invasive pest of ripening soft fruit expanding its geographic range and damage potential. Journal of Integrated Pest Management 2: 1-7.
  • Yassin A (2008). Molecular and morphometrical revision of the Zaprionus tuberculatus species subgroup (Diptera: Drosophilidae), with descriptions of two cryptic species. Annals of the Entomological Society of America 101: 978-988.
  • Yassin A, David JR (2010). Revision of the Afrotropical species of Zaprionus (Diptera, Drosophilidae), with descriptions of two new species and notes on internal reproductive structures and immature stages. ZooKeys 51: 33-72.