Characterization of branchial Na,K-ATPase from three freshwater fish species (Oreochromis niloticus, Cyprinus carpio, and Oncorhynchus mykis)

Bu çalışmada farklı ekolojik gereksinimleri olan üç farklı tatlı su balığında (O. niloticus, C. carpio, O. mykiss) solungaç Na,KATPaz enzimlerinin karakterizasyonu yapılmıştır. Aynı zamanda solungaçlarda Na+, K+ ve Cl- düzeyleri de belirlenmiştir. En yüksek Na,K-ATPaz aktivitesi 100 mM Na+, 20 mM K+, 3-4 mM Mg+2, 1 mM Ouabain derişimlerinde gözlenmiştir. O. mykiss’da (1,07 μmol Pi/mg prot/h), O. niloticus (7,25 μmol Pi/mg prot/h) ve C. carpio (7,44 μmol Pi/mg prot/h) ile karşılaştırıldığında gözlenen en düşük maximal hız (Vmax) değeri, solungaçlardaki yüksek iyon düzeyleri ile ilişkilendirilebilir. Buna karşılık, O. niloticus ve C. carpio’da yarı Vmax değerine sahip substrat derişimleri (Km) ise, benzer Vmax değerlerine karşılık farklılık göstermektedir. O. niloticus’da C. carpio’ya oranla gözlenen düşük Km değerinin, değişen ortam koşullarında O. niloticus’un daha dirençli olmasından kaynaklanabileceği bildirilmiştir.

Tatlı su balıklarındaki (Oreochromis niloticus, Cyprinus carpio, Oncorhynchus mykiss) solungaç Na,K-ATPaz enzimlerinin karakterizasyonu

Branchial Na,K-ATPase activity was characterized in 3 freshwater fish species (Oncorhynchus mykiss, Oreochromis niloticus, and Cyprinus carpio) with different ecological needs. Na+, K+, and Cl- concentrations in the gills were also measured. The maximal Na,K-ATPase activity was observed at 100 mM Na+, 20-40 mM K+, 3-4 mM Mg2+, and 1 mM ouabain. The maximal velocity (Vmax) of Na,K-ATPase isolated from O. mykiss (1.07 μmol Pi/mg prot/h) was lower than that isolated from O. niloticus (7.25 μmol Pi/mg prot/h) and C. carpio (7.44 μmol Pi/mg prot/h). Nevertheless, Na+, K+, and Cl- levels in O. mykiss were higher than the others. The lowest Vmax value can be attributed to the highest ion concentrations in the gills of O. mykiss. However, substrate concentrations, which give half of Vmax (Km) of Na,K-ATPase in the gills of O. niloticus and C. carpio, were different although they exhibited similar Vmax values. The low Km value in the gills of O. niloticus compared to C. carpio may be related to high resistance to changing environmental factors.

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