Nusret AKYURTLAKLI, Maryam RASSOULZADEGAN

An investigation on the toxic effects of malathion (Organophosphate insecticide) on the Daphnia magna straus, 1820 (Crustacea, Cladocera)

Bu çalışmada Daphnia magna yavrularında (£ 24 saatlik) teknik düzeydeki (%95) malathion ve ticari (%25) malathionun toksik etkileri statik, akut toksisite denemeleriyle (24 ve 48 saatlik) araştırılmıştır. D. magna kültürü ve toksisite denemeleri, 24±1¼C sıcaklık ve 16 saat ışık / 8 saat karanlık fotoperiyodunda düzenlenmiştir. Sağlıklı bir populasyon elde etmek için, her bir dişi ferdin hayatı boyunca meydana getirdiği yavruların sayısı da araştırılmıştır. 24 / 48 saat %50 etki konsantrasyonunun (EC50) saptanması için dafnidlerin hareket durumu dikkate alınmıştır. EC50 değerleri, istatistiksel olarak probit metodu ile 24 / 48 saat sırasıyla, teknik malathion için 0.8 ve 0.028 ppm., ticari malathion için 0.11 ve 0.003 ppm bulunmuştur.

Malathion (Organik fosforlu insektisit)' un Daphnia magna Straus, 1820 (Crustacea, Cladocera) üzerindeki toksik etkilerinin araştırılması

The toxic effects of technical grade (95 %) malathion and commercial (25 %) malathion were investigated on young (£ 24 h old) Daphnia magna in static acute toxicity tests (24 and 48 h). The culture of D. magna and toxicity tests were performed at 24 $pm$ 1 $circ$C with a photoperiod of 16 h light and 8 h dark. To obtain a healthy population, the fecundity of each female in their life cycle was also investigated. The immobilization of daphnids was considered for determination of the 24 h / 48 h effective concentration (50 %). The EC50 values were found by the probit method 24 h /48 h for technical malathion as 0.8 and 0.028 ppm., and for commercial malathion as 0.11 and 0.003 ppm respectively.

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1. Cook, J.L., Bauman, P., Jackman, J. A., and Stevenson, D. Pesticide characteristics that affect water quality. Farm Chemicals HandbookÕ95, Meister Publishing Co, Willoughby, OH, 429 pp, 1995.
2. Forget, J., Pavillon, J. F., Beliaeff, B., Bocquene, G. Joint action of pollutant combinations (Pesticides and Metals) on survival (LC50 Values) and acetylcholinesterase activitiy of Tigriopus brevicornis (Copepoda, Harpacticoida). Environmental Toxicology and Chemistry, 18(5):912-18, 1999.
3. Azmi, M. A., Naqvi, S. N. H., Ahmad, Ü., Tabassum , R., Anbreen, B. Toxicity of neem leaves extract (NLx) compared with malathion (57E.C.) against late 3rd instar larvae of Culex fatigans (Wild strain) by WHO method. Tr. J. of Zoology, 22: 213-218, 1998.
4. Öztürk, S. Tarım İlaçları, AK Basımevi, İstanbul, s. 57, 1997.
5. Dodson, S. L., Hanazato, T. Commentary on effects of anthropogenic and natural organic chemicals on development, swimming behaviour and reproduction of Daphnia, a key member of aquatic ecosystems. Environ Health Perspect 103 (Suppl 4):7- 11, 1995.
6. Peters, R. H., De Bernardi, R. Daphnia. Mem. Dell. Ist. 1tal. Idrobiol.,45 Verbania Pallanza, 1987.
7. Green, J. In: A biology of Crustacea (Aspects of Zoology). 39-49, 62-87, 1961.
8. Martinez-Feronino-Fernando, Espinosa-Chavez-Felix, Villasenor- Rafael, Effect of culture volume and adult density on the neonate production of D. magna, as a test organism for aquatic toxicity tests. Environmental Toxicology, 15 (3) 155-159 2000.
9. Persoone, G, and Jansson, C. R, Freshwater invertebrate Toxicity tests, in Handbook of Ecotoxicology, Vol. 1, p.51-65, 1993.
10. Organization for Economic Cooperation and Development (OECD) (1984). Daphnia spp., acute immobilization and reproduction test, OECD Guideline for testing Chemicals, No: 202, Geneva.
11. Lilius, H., Isomaa, B., Holmstram, T. A comparison of the toxicity of 50 reference chemicals to freshly isolated rainbow trout hepatocytes and Daphnia magna. Aquatic Toxicology 30:47-60, 1994.
12. Gustavo, D., Rossini, B., and Ronco, A. E. Acute toxicity bioassay using Daphnia obtusa as a test organism. Environmental Toxicology and Water Quality: An International Journal, 11:255- 58, 1996.
13. Nelson, S. M., Roline, R. A. Evaluation of the sensitivity of rapid toxicity tests relative to Daphnia acute lethality tests. Bull. Environ. Contam. Toxical. 60:292-99, 1998.
14. Alberdi, J. L., Saenz, M. E., Dimarzio, W. D., Tortorelli, M. C. Comparative acute toxicity of two herbicides, paraquat and glyphasate, to D. magna and D. spinulata. Bull Environ. Contam. Toxicol. 57:229-35, 1996
15. Al-omar, M. A., Hassan, N. A. Simple and Rapid Method for the Detection of Early Signs of Toxicity in Daphnia magna Straus, Bull. Environ. Contam. Toxicol. 65: 553-559, 2000.
16. Bliss,C.I .The calculation of dosage mortality curve. Ann.appl.Bill.,22:134-167,1935.
17. Alpbaz, A. G., Temelli, B., Korkut, A. Y. Su piresi (D. magna Straus) yetiştiriciliği üzerinde bir araştırma. E.Ü.S.Ü. Yüksek Okulu, Bornova, İzmir. 16-76, 1989.
18. Gürbüz, H., nalan, S. K. Su piresi ( D. magna)'nın farklı kültür ortamlarında yetiştirilmesi. Tr. J. of Veterinary and Animal Sciences, 22: 237-242, 1998.
19. Elendt, B. P., Bias, W. R. Trace nutrient deficiency in Daphnia magna cultured in standard medium for toxicity testing. Effects of the optimization of culture conditions on life history parameters of D. magna. Wat. Res. Vol. 24, No: 9, p:1157-1167, 1990.
20. Sayım, F.,Akyurtlaklı,N. Malathion (Organik fosforlu insektisit)'un 25. Çevre Rana ridibunda (Amphibia:Anura) larvaları üzerindeki akut toksisitesi. E.Ü.Su ürünleri Dergisi ,cilt :16,sayı: 1-2:19-29,1999.
21. Enserink, L., Luttmer, W. and Diepeveen, H.M. Reproductive strategy of Daphnia magna affects the sensitivity of its progency in acute toxicology tests. Aquatic Toxicology, 17, 15-26, 1990.
22. Baird, D. J., Barber, I., Bradly, M., Calow, P. and Soares, A. M. V. M. The Daphnia bioassay: A critique hydrobiologia. 188/189, 403- 406, 1989.
23. Connell, D., Lam, P., Richardson and Wu, R. Introduction to Ecotoxicology. Blackwell Science, p: 111, 1999.
24. Rawash, IA., Gaaboub, IA, El-Gayar, E M., El-Shazli, A Y. Standard curves for nuvacron, malathion, sevin, DDT and kelthane tested against the mosquito Culex pipiens and the microcrustacean D. magna Straus. Toxicology May 4:2, 133-44, 1975.
25. Canyurt, M. A. Malathion'un Pulaterina (Mugil capito Cuvier, 1829) için lethal konsantrasyonu (LC50) üzerine araştırmalar. Ege Üniv. Ziraat Fak. Dergisi Cilt 26 Sayı 2, 1989
26. Ankley, G. T., Dierkes, J. R., Jensen, D. A Piperonyl butoxide as a tool in aquatic toxicological research with organophosphate insecticides. Ecotoxicology and Environmental Safety 21, 266-74, 1991.
27. Gaizick, L., Gupta, G., Bass, E. Toxicity of Chlorypyrifos to Rana pipiens Embryos. Bull. Environ. Contam. Toxicol. 66: 386-391, 2001.