The geographic range of Piseinotecus soussi Tamsouri, Carmona, Moukrim & Cervera, 2014 is here updated, since it was found in two new localities along the Tyrrhenian coast of the Mediterranean Sea. Furthermore, to confirm the identity of specimens recorded, a DNA barcoding approach was carried out using mitochondrial cytochrome c oxidase subunit I (COI) as it is the molecular marker mostly used in barcoding analysis in nudibranchs.