Meltem KÖKDENER1, Nevran Eylem AKMAN GÜNDÜZ, Ünal ZEYBEKOĞLU

The impact of chilling on selected attributes of the blowfly, Calliphora vicina (Robineau-Desvoidy, 1830) (Diptera: Calliphoridae), under laboratory conditions

The purpose of this study was to evaluate the effects of four different chilling periods, varying from 24 to 120 h, on the mean survival rate, larval and pupal development durations and the pupal and adult weight of Calliphora vicina (Robineau-Desvoidy) (Diptera: Calliphoridae) under laboratory conditions at 4 oC in 2018 at Ondokuz Mayıs University. Chilling reduced the survival rate of the pupae of all developmental stages. The adult eclosion rate of first instar larvae and pupae was lower, especially after 72 and 120 h chilling periods, but it increased for the second instar, third instar and postfeeding larvae with increasing chilling period. In addition, the larval development period was increased by increased chilling period for all developmental stages except for postfeeding larvae for 120 h. Pupal and adult weight were also affected by chilling period, and the postfeeding larval and pupal stages were the most intolerant groups to chilling. Overall, the effects of chilling on the life history parameters of C. vicina were substantial and should be considered to provide a more reliable estimate of postmortem interval.

Keywords:

Calliphora vicina, chilling period development duration, survival rate, weight,

PDF

___

Açıkgöz HN, Köse SK, Açıkgöz A (2017). Determination of early colonizer urban blowfly species using small bait traps on a university campus. Pakistan Journal of Zoology 49 (1): 121- 128. doi: 10.17582/journal.pjz/2017.49.1.117.124
Ahmed AB, Samson JG (2016). Estimation of postmortem interval using the blowfly Phaenicia (Lucilia) sericata (Diptera: Calliphoridae) in Kaduna, Northern Nigeria. International Journal of Research in Medical Sciences 4 (8): 3417-3420. doi: 10.18203/2320-6012.ijrms20162304
Alipour H, Shahriari-Namadi M, Raz A, Moemenbellah-Fard MD (2018.) Cold-preservation of Lucilia sericata (Diptera: Calliphoridae) pupae and adult products as a new venture to adults rearing. Journal of Experimental Biology and Agricultural Sciences 6 (3): 544-549. doi: 10.18006/2018.6(3).544.549
Amendt J, Krettek R, Zehner R (2004). Forensic entomology. Naturwissenschaften 91 (2): 51-65. doi: 10.1007/s00114-003- 0493-5
Amendt J, Campobasso CP, Gaudry E, Reiter C, LeBlanc HN et al. (2007). Best practice in forensic entomology–standards and guidelines. International Journal of Legal Medicine 121: 90- 104. doi: 10.1007/s00414-006-0086-x
Ames C, Turner B (2003). Low temperature episodes in development of blowflies: implications for postmortem interval estimation. Medical and Veterinary Entomology 17: 178-186. doi: 10.1046/j.1365-2915.2003.00421.x
Anderson GS, VanLaerhoven SL (1996). Initial studies on insect succession on carrion in southwestern British Columbia. Journal of Forensic Science 41: 617-625. doi: 10.1529/JFS13964
Bucher GE, Cameron B, Mac JW, Wilkes A (1948). Studies on the housefly (Musca domestica L.). II. The effects of low temperatures on laboratory-reared puparia. Canadian Journal of Research Section D Zoological Sciences 26: 26-56. doi: 10.1139/cjr48d-004
Carriço C, Caetano RL, Barbosa JV, Pinto ZT (2019). Cooling and freezing effects on development of flies of forensic importance. Entomo Brasilis 12 (2): 57-62. doi: 10.12741/ebrasilis.v12i2.823
Dekeirsschieter J, Verheggen FJ, Gohy M, Hubrecht F, Bourguignon L et al. (2009). Cadaveric volatile organic compounds released by decaying pig carcasses (Sus domesticus L.) in different biotopes. Forensic Science International 189 (1-3): 46-53. doi: 10.1016/j.forsciint.2009.03.034
Donovan SE, Hall MJR, Turner BD, Moncrieff CB (2006). Larval growth rates of the blowfly, Calliphora vicina, over a range of temperatures. Medical Vetarinary Entomology 20: 106-114. doi: 10.1111/j.1365-2915.2006.00600.x
Goff ML, Flynn MM (1991). Determination of postmortem interval by arthropod succession: a case study from the Hawaiian Island. Journal of Forensic Science 36: 607-614. doi: 10.1097/00000433-198809000-00009
Introna F, Campobasso CP, Di Fazio A (1998). Three case studies in forensic entomology from Southern Italy. Journal of Forensic Science 43 (1): 210-214. doi: 10.1520/JFS16117J
Johl HK, Anderson GS (1996). Effects of refrigeration on development of the blow fly, Calliphora vicina (Diptera: Calliphoridae) and their relationship to time of death. Journal of the Entomological Society of British Columbia 93: 93-98.
Kökdener M (2012). Adli entomolojide kullanılan sinek türlerinin Samsun’da mevsimlere göre durumunun belirlenmesi. PhD, İstanbul University, İstanbul, Turkey (in Turkish). Kökdener M (2016). Application of entomology in forensic sciences. Turkish Bulletin of Entomology 6 (3): 269-275. doi: 10.16969/ teb.90382
Leopold RA (2000). Short- term cold storage of house fly (Diptera: Muscidae) embryos: survival and quality of subsequent stages. Annals of the Entomological Society of America 93 (4): 884- 889. doi: 10.1603/0013-8746(2000)093[0884:STCSOH]2.0. CO;2
Leopold RA, Rojas RR, Atkinson PW (1998). Post pupariation cold storage of three species of flies: increasing chilling tolerance by acclimation and recurrent recovery periods. Cryobiology 36: 213-224. doi: 10.1006/cryo.1998.2081
Magni PA, Dhaliwal S, Dadour IR (2016). Effect of continuous and cyclic exposure to a cold environment on the development of larvae of Lucilia sericata (Diptera: Calliphoridae) in different sized larval masses. Journal of Medical Entomology 53 (4): 1-8. doi:10.1093/jme/tjw036
Myskowiak JB, Doums C (2002). Effects of refrigeration on the biometry and development of Protophormia terraenovae (Robineau–Desvoidy) (Diptera: Calliphoridae) and its consequences in estimating post-mortem interval in forensic investigations. Forensic Science International 125: 254-261. doi: 10.1016/S0379-0738(02)00003-8
Pellitero JM, Saloña Bordas MI (2007). Development of Calliphora vicina Robineau-Desvoidy (Diptera, Calliphoridae) under starvation conditions. Boletín Sociedad Entomológica Aragonesa 141: 413-417. doi: 10.1007/s00414-014-1068-z
Salimi M, Rassi Y, Oshaghi M, Chatrabgoun O, Limoee M, Rafizadeh S (2018). Temperature requirements for the growth of immature stages of blowflies species, Chrysomya albiceps and Calliphora vicina, (Diptera: Calliphoridae) under laboratory onditions. Egyptian Journal of Forensic Sciences 8: 28-36. doi: 10.1186/s41935-018-0060-z
Sanford MR (2017). Insects and associated arthropods analyzed during medicolegal death investigations in Harris County, Texas, USA: January 2013- April 2016. PLoS ONE 12 (6): e0179404. doi: 10.1371/journal.pone.0179404
Saunders DS, Hayward SAL (1998). Geographical and diapause- related cold tolerance in the blow fly, Calliphora vicina. Journal of Insect Physiology 44 (7-8): 541-551. doi: 10.1016/S0022- 1910(98)00049-3
Schoenly K, Reid W (1987). Dynamics of heterotrophic succession in carrion arthropod assemblages: discrete seres or a continuum of change? Oecologia 73: 192-202. doi: 10.1007/BF00377507
Sumodan PK (2002). Insect detectives. Resonance 7 (8): 51-58.
Warren JA, Anderson GS (2013). The development of Protophormia terraenovae (Robineau-Desvoidy) at constant temperatures and its minimum temperature threshold. Forensic Science International 233: 374-379. doi: 10.1016/j.forsciint.2013.10.012