Profiles of serum protein fractions pre-treatment and post-treatment in lambs with pica disorder

Profiles of serum protein fractions pre-treatment and post-treatment in lambs with pica disorder

Objective:The present study was planned to examine the changes in serum protein fractions of lambs with pica symptoms with electrophoresis.Materials and Methods:The three study groups were the control group that included healthy lambs, pre-treatment and post-treatment groups that included lamb with pica. Lambs with pica symptoms were then treated with a single capsule mineral complex orally and single dose vitamin complex. Serum protein fractions in blood samples were determined with cellulose-acetate electrophoresis. Results:It was determined that in terms of concentration, total protein, albumin and β-globulin, levels did not change, albumin percentage levels increased with pica, however reached control group levels with treatment and α1-Globulin and α2-Globulin levels reduced after treatment. Based on % gr, it was determined that α1-Globulin and β-Globulin did not change and α2-Globulin decreased. ɣ-Globulin level and percentage increased after treatment.Conclusion:Profiles of serum protein fractions may play an important role in the etiology of pica; it was suggested that the identification of serum protein fractions should be considered as a beneficial method in veterinary clinic for the detection, treatment and follow-up of certain diseases and certain nutrition-related conditions

___

  • Adkins JN, Varnum SM, Auberry KJ, et al. Toward A Human Blood Serum Proteome: Analysis By Multidimensional Separation Coupled With Mass Spectrometry. Mol Cell Proteomics 2002; 1:947–955.
  • Akdoğan KA, Bakirel U, Gönül R., Tan H, Vurusaner C. Serum protein electrophoresis in dogs with intestinal parasites. Turk J Vet Anim Sci 1999; 23: 457-459.
  • Akgul Y, Agaoglu ZT, Kaya A, Salin T. The relationship between the syndromes of wool eating and alopecia in akkaraman and morkaraman sheep fed corn silage and blood changes (haematological, biochemical and trace elements). Israel J Vet.Med 2000;56(1):23–37.
  • Apaydin, B, Dede S. Electrophoretic Profile of serum protein fractions from sheep naturally infected with babesia ovis. Re Méd Vét 2010; 161(2): 57-60.
  • Aytekin I, Onmaz AC, Serap UA, Vehbi G, Osman K. Changes in serum mineral concentrations, biochemical and hematological parameters in horses with pica. Biol Trace Elem Res 2011; 139: 301–307.
  • Aytekin I, Kalınbacak A. Calcium, phosphorus, magnesium, copper, zinc and ıron levels in soil eating habitats grown in Afyon region (in Turkish). Atatürk Üniversity J Vet Sci 2008; 3 (2): 34–42.
  • Elshahawy II, Aly MA. Some studies on deviated appetite (pica) in cattle. Alexandria Journal of Veterinary Sciences 2016; 51 (1): 97-101.
  • Erdal ME. Investigating cancerous serum protein levels and the frequency of some haptoglobin types with the Gel Disc Electrophoresis method (PhD Thesis) 1987. Diyarbakir: Dicle University, Institute of Medical Sciences.
  • Faye B, Bengoum M. Trace element status in camels. Biol Trace Element Re 1994; 41: 1-11.
  • Firyal S. Pica (Depraved Appetite; Allotrophagia) in domestic animals and man (Extension Article). Pakistan Vet J 2007; 27(4): 208-210.
  • Güllü A and Dede S. The Serum Protein Fractions in Thymoquinone Treated Rats. Afr J Tradit Complement Altern Med 2016; 13(6): 27–31
  • Jacobs JM, Adkins JN, Qian WJ, Liu T., Shen Y., Camp D. G., Smith R. D.: Utilizing human blood plasma for proteomic biomarker discovery. J Proteome Res 2005, 4 (4), 1073–1085.
  • Karagu¨l, H., Altintas, A., Fidanci, U. R., & Sel, T. (2000). Clinical biochemistry (pp. 161–164). Medisan Inc. Ankara.
  • Metzler DE, Metzler CM. Biochemistry: The chemical reactions of living cells. 2nd. Ed. Academic Press; 2001. p. 58.
  • Onat, T., Kaya, E., & Sozmen, E. Y.: Human biochemistry 2002 (pp. 184–218). Ankara: Palme Publishing.Singh K. P, Malik K. S., Sarup S.: Haemato-biochemical studies in camel suffering from pica. Indian J Vet Med 1986, 6(2), 79–81
  • Smith, B. P. large animal internal medicine, 5th edition 2015. Page 152. Mosby, ELSEVIER.
  • Şahin T., Cimtay I., Aksoy G.: Investigations on some biochemical parameters in lambs with pica and in healthy lambs. Turk J Vet Anim Sci 2001, 25, 603–606.
  • T. E. C., Jr.: The Origin of The Word Pica. Pediatrics 1969, 44 (4), 548.
  • Walker H. K., Hall W. D., Hurst J. W.: History, physical, and laboratory examinations 3rd edition: Serum albumin and globulin (Busher, J. T.) University School Of Medicine, Atlanta, Georgia Boston 1990, 101. Chapter.
  • Wıjnen P. A., Van Dieijen-Vısser M. P.: Capillary electrophoresis of serum proteins. reproducibility, comparison with agarose gel electrophoresis and a review of the literature. Eur J Clin Chem 1996, 34, 535-545.
  • Yuksek V., Dede S., Ceylan E.: The Electrophoretical determination of serum protein fractions in lycopene treated experimental diabetic rats. Cell Biochem. Biophys 2013, 67(3), 1283-1289.
  • Nikvand A. A, Rashnavadi M., Tabandeh M. R.: A Study of pica in cattle in Iran. J Vet Behav 2018, 23, 15-18