Mani KANNAN, Muthukalingan KRISHNAN, Sankarappan ANBALAGAN, Vimalanathan PRASANNA ARUN

The midgut bacterial fora of the hard tick Hyalomma anatolicum (Acari: Ixodidae) from South India as determined by molecular analyses

The midgut bacterial fora of post-blood meal and starved or digested blood meal of hard ticks (Hyalomma anatolicum )from goats were analyzed using BOX PCR, ERIC PCR and 16S rDNA gene sequencing. BOX PCR and ERIC PCR showed dissimilarband patterns between microbial isolates from the midgut of the ticks. In total, 9 bacterial species belonging to 2 genera (Bacillus andStaphylococcus) were identifed in the midgut of both types (post-blood meal and digested blood meal) of tick by using 16S rDNA genesequencing. The majority of the midgut bacterial species were Staphylococcus sp. (90%) and there was a high frequency of 3 species ofStaphylococcus ( S. chromogenes, S. epidermidis, and S. gallinarum ). Phylogenetic analysis based on 16S rDNA gene sequences showedthat all midgut isolates were monophyletic and showed high-frequency relationships with S. chromogenes and S. epidermidis. Furtherstudy is needed to understand the relationship between midgut bacterial community and tick pathogen interaction.

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