The effects of in ovo ınsulin-like growth factor-1 on embryonic development of musculus longus colli dorsalis in japanese quail
Özet: Bu çalışmanın amacı; Japon bıldırcını embriyolarının m. longus colli dorsalis (m. spinalis thoracis) kasının thoracic kısmının histolojik gelişiminde, insulin-benzeri büyüme faktörü-Tin etkilerini değerlendirmekti. Rekombinant insan IGF-l'i (rhIGF-1), yumurtanın küt ucunda, dental drill bit ile açılan delikten, bir defa olmak üzere 100 ng/her bir embriyo oranında chorio-allantoik membrana girmeksizin enjekte edildi. Histolojik değerlendirmeler için dokular, embriyonik gelişimin 7 ile 16. günleri arasında günlük olarak toplandı. RhIGF-1 uygulamasının embriyonik gelişimin 7, 9, 10, 11 ve 13. günlerinde kas tellerinin sayı ve çapında anlamlı bir artışa neden olduğu gözlendi (P < 0,001). Sonuç olarak; rhIGF-1'in Japon bıldırcını embriyolarının iskelet kası gelişimini hızlandırdığı sonucuna varıldı.
Japon bıldırcınlarında musculus longus colli dorsalis' in embriyonik gelişimiüzerinde in ovo insulin-benzeri büyüme faktörü-1'in etkileri
Abstract: The objective of the present study was to evaluate the effects of insulin-like growth factor-1 (IGF-1) on the histological development of the thoracic part of the m. longus colli dorsalis (m. spinalis thoracis) in Japanese quail embryos. A single in ovo dose of recombinant human IGF-1 (rhIGF-1) (100 ng embryo"1) was administered through the blunt end of eggs via a single hole made with a dental drill bit, without penetrating the chorioallantoic membrane. For histological evaluation, the embryos were collected daily from days 7 to 16 of embryonic development (E). In ovo administration of rhIGF-1 increased the diameter of muscle fibers on E7, 9, 10, 11, and 13. Additionally, in ovo rhIGF-1 also increased the number of muscle fibers (P < 0.001). It was concluded that •• rhIGF-1 accelerated skeletal muscle development in the quail embryos.
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