The Effects of Aflatoxin and Sodium Bentonite Combined and Alone on Some Blood Electrolyte Levels in Broiler Chickens

The aim of the study is to determine the effects of aflatoxin and sodium bentonite given alone and in combination on some of blood electrolyte levels in broiler chicks. For this aim, 72 male one-day-old Avian broiler chicks were used. The animals were separated into 6 groups, as one control (Group 1) and the others trial (Groups 2 to 6). While commercial feed was given to Group 1 (control), the other groups received feed containing 0.25% sodium bentonite, 0.50% sodium bentonite, 1 ppm aflatoxin (approximately, 85% B1, 10% B2, 3% G1 and 2% G2), 1 ppm aflatoxin with 0.25% sodium bentonite and 1 ppm aflatoxin with 0.50% sodium bentonite (Groups 2-6), respectively, for 45 days. Blood samples were taken on the 15th, 30th and 45th days of the study and the sodium, potassium, chloride, phosphorus and calcium levels in serum were analyzed. At the end of the study, it was determined that aflatoxin caused a decrease in calcium and phosphorous levels. On the other hand, it was observed that sodium bentonite in the feed caused a decrease in both of these electrolyte levels, even though these decreases were higher in the groups that received aflatoxin alone.

The Effects of Aflatoxin and Sodium Bentonite Combined and Alone on Some Blood Electrolyte Levels in Broiler Chickens

The aim of the study is to determine the effects of aflatoxin and sodium bentonite given alone and in combination on some of blood electrolyte levels in broiler chicks. For this aim, 72 male one-day-old Avian broiler chicks were used. The animals were separated into 6 groups, as one control (Group 1) and the others trial (Groups 2 to 6). While commercial feed was given to Group 1 (control), the other groups received feed containing 0.25% sodium bentonite, 0.50% sodium bentonite, 1 ppm aflatoxin (approximately, 85% B1, 10% B2, 3% G1 and 2% G2), 1 ppm aflatoxin with 0.25% sodium bentonite and 1 ppm aflatoxin with 0.50% sodium bentonite (Groups 2-6), respectively, for 45 days. Blood samples were taken on the 15th, 30th and 45th days of the study and the sodium, potassium, chloride, phosphorus and calcium levels in serum were analyzed. At the end of the study, it was determined that aflatoxin caused a decrease in calcium and phosphorous levels. On the other hand, it was observed that sodium bentonite in the feed caused a decrease in both of these electrolyte levels, even though these decreases were higher in the groups that received aflatoxin alone.

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  • Huff, W.E., Kubena, L.F., Harvey, R.B., Hagler, W.M., Swanson, S.P., Phillips, T.D., Creger, C.R.: Individual and combined effects of aflatoxin and deoxynivalenol (DON, Vomitoxin) in broiler chickens. Poult. Sci., 1986; 65: 1291-1298.
  • Dalvi, R.R.: An overview of aflatoxicosis of poultry: Its
  • characteristics, prevention and reduction. Vet. Res. Commun., 1986; 10: 429-443.
  • Kaya, S.: Küflenmeden flüpheli yem ve yem hammaddelerinde aflatoksinler. Ankara Üniv. Vet. Fak. Derg., 1985; 32: 1-12. 4. Oğuz, H., Kurtoğlu, F., Kurtoğlu, V., Birdane, Y.O.: Evaluation of biochemical characters of broiler chickens during dietary aflatoxin (50 and 100 ppb) and clinoptilolite exposure. Res. Vet. Sci., 2002; 73: 101-103.
  • Oğuz, H., Kurtoğlu, V., Coflkun, B.: Preventive efficacy of
  • clinoptilolite in broilers during chronic aflatoxin (50 and 100 ppb) exposure. Res. Vet. Sci., 2000; 69: 197-201.
  • Quezeda, T., Cuellar, H., Jaramillo-Juarez, F., Valdivia, A.G., Reyes, J.L.: Effect of aflatoxin B1on the liver and kidney of broiler chickens during development. Comp. Biochem. Physiol. Part C., 2000; 125: 265-272.
  • Ortatatlı, M., Çiftçi, M.K., Tuzcu, M., Kaya, A.: The effects of aflatoxin on the reproductive system of roosters. Res. Vet. Sci., 2002; 72: 29-36.
  • Harvey, R.B., Kubena, L.F., Phillips, T.D.: Evaluation of
  • aluminosilicate compounds to reduce aflatoxin residues and toxicity to poultry and livestock: a review report. Sci. Total. Environ., 1993; 2: 1453-1457.
  • Phillips, T.D., Clement, B.A., Kubena, L.F., Harvey, R.B.:
  • Detection and detoxification of aflatoxins: prevention of aflatoxicosis and aflatoxin residues with hydrated sodium calcium aluminosilicate. Vet. Hum. Toxicol., 1990; 32: 15-19.
  • Schell, T.C., Lindemann, M.D., Kornegay, E.T., Blodgett, D.J.: Effects of feeding aflatoxin-contaminated diets with and without clay to weanling and growing pigs on performance, liver function, and mineral metabolism. J. Anim. Sci., 1993; 71: 1209-1218.
  • Diaz, D.E., Hagler, W.M. Jr., Hopkins, B.A., Whitlow, L.W.: Aflatoxin binders I: in vitro binding assay for aflatoxin B1by several potential sequestering agents. Mycopathologia, 2002; 156: 223-226.
  • Demet, Ö., Oğuz, H., Çelik, İ., Nizamlıoğlu, F.: Pirinçte aflatoksin üretilmesi. Vet. Bil. Derg., 1995; 11: 19-23.
  • Shotwell, O.L., Hesseltine, C.W., Stubblefield, R.D., Sorenson, W.G.: Production of aflatoxin on rice. Appl. Microbiol., 1966; 14: 425-428.
  • Nabney, J., Nesbit, B.F.: A spectrophotometric method for determination of the aflatoxins. Analyst, 1965; 3: 155-159.
  • Anon.: SPSS 9.05 for Windows Package Program.
  • Oğuz, H., Keçeci, T., Birdane, Y.O., Onder, F., Kurtoğlu V.: Effect of clinoptilolite on serum biochemical and haematological characters of broiler chickens during aflatoxicosis. Res. Vet. Sci., 2000; 69: 89-93.
  • Oğuz, H., Kurtoğlu, V.: Effect of clinoptilolite on performance of broiler chickens during experimental aflatoxicosis. Br. Poult. Sci., 2000; 41: 512-517.
  • Glahn, R.P., Beers, K.W., Bottje, W.G., Wideman, R.F., Huff, W.E., Thomas, W.: Aflatoxicosis alters avian renal function, calcium, and vitamin D metabolism. J. Toxicol. Environ. Health., 1991; 34: 309-321.
  • Keçeci, T., Oğuz, H., Kurtoğlu, V.: Effects of polyvinylpolypyrrolidone, synthetic zeolite and bentonite on serum biochemical and hematological characters of broiler chickens during aflatoxicosis. Br. Poult. Sci., 1998; 39: 452-458.
  • Huff, W.E., Harvey, R.B., Kubena, L.F., Rottinghaus, G.E.: Toxic synergism between aflatoxin and T-2 toxin in broiler chickens. Poult. Sci., 1998; 67: 1418-1423.
  • Stul, M.S., Vliers, D.P., Uytterhoven, J.B.: In vitro adsorption- desorption of phenethylamines and phenylimidazoles by a bentonite and a resin. J. Pharm. Sci., 1984; 73: 1372-1375.
  • Smith, E.E., Kubena, L.F., Braithwaite, C.E., Harvey, R.B., Phillips, T.D., Reine, A.H.: Toxicological evaluation of aflatoxin and cyclopiazonic acid in broiler chickens. Poult. Sci., 1992; 71: 1136-1144.
  • Giroir, L.E., Huff, W.E., Kubena, L.F., Harvey, R.B., Elissalde, M.H., Witsel, D.A., Yersin, A.G., Ivie, G.W.: The individual and combined toxicity of kojic acid and aflatoxin in broiler chickens. Poult. Sci., 1991; 70: 1351-1356.
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK