Asiye İzem SANDAL, Mehmet Can GÜNDÜZ, Sinem Özlem ENGİNLER, Alper BARAN, Çağatay TEK, Ramazan ARICI, Israa Faris MOHAMMED, Elif Merve ÇINAR, Özen Banu ÖZDAŞ, Ezgi ERTÜRK

The effect of oviductal cells on in vitro maturation of canine oocytes in diferent culture media

The aims of this study were to investigate the effect of oviductal cells on in vitro maturation (IVM) of canine oocyte in TissueCulture Medium 199 (TCM-199) or synthetic oviductal fuid (SOF) supplemented with bovine serum albumin (BSA) or fetal calfserum (FCS) and to compare the maturation rates of oocytes from the diestrus and anestrus stages. Following ovariohysterectomy, 13pairs of ovaries were collected from bitches in anestrus (n = 10) or diestrus (n = 3) and oocytes were harvested by slicing. Te oviductswere fushed with TCM-199 containing 10% FCS and were scraped and squeezed into a tube in order to obtain oviductal cells. Selectedoocytes were divided into groups for IVM over 48 h for each of the diestrus and anestrus stages as follows: Group Ia, SOF+BSA; GroupIb, SOF+BSA+oviductal cells; Group IIa, SOF+FCS; Group IIb, SOF+FCS+oviductal cells; Group IIIa, TCM-199+BSA; Group IIIb,TCM-199+BSA+oviductal cells; Group IVa, TCM-199+FCS; and Group IVb, TCM-199+FCS+oviductal cells. Aferwards, oocytes werefxed with acetic acid-ethyl alcohol and stained with aceto-orcein to determine nuclear maturation. When compared between anestrusand diestrus stages for all parameters (undetermined nuclear material, germinal vesicles, germinal vesicle break down, metaphase I,metaphase II, and degenerated) in diferent media, the diferences were found to be signifcant statistically in Group IIa (22.9%) andGroup IIIb (35.7%) for the germinal vesicle stage (P < 0.05) as compared to the other groups. In conclusion, in the oocytes obtained frombitches in diestrus and anestrus supplemented with FCS or BSA in SOF medium without oviductal cells, more positive effects were seenon canine oocyte maturation than with TCM-199 medium supplemented with same protein sources and oviductal cells.

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