Aydın HİM, Nimet Burcu DENİZ, Mehmet Emin ÖNGER

The effect of caffeine on neuron number of rats exposed to 900-MHz electromagnetic field

Previous studies have shown that the electromagnetic field (EMF) emitted by cell phones decreased the number of neurons in some parts of the brain. It was also shown that caffeine protects the nerve cells. This study examines if caffeine has any effects on preventing neuron loss in rats exposed to EMF. Rats were exposed to a 900-MHz EMF for 60 min a day for 28 days. The rats that were exposed to EMF were fed 1 mg/L caffeinated water instead of tap water. The rats in the sham group were put inside the exposure chamber but were not exposed to EMF. The changes in the hippocampal and cerebellar neuron numbers were examined with stereological histological methods. EMF application caused a significant decrease in the number of the cerebellar Purkinje neurons and the hippocampal pyramidal neurons. In the group that received caffeine with EMF exposure, the decrease in neuron number was prevented in both the hippocampus and the cerebellum. The results show that caffeine protects the cerebellum and the hippocampus from neural damage induced by EMF exposure.

Keywords:

Electromagnetic field, rat Purkinje cell, pyramidal neuron, stereology,

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Hossmann KA, Hermann DM. Effects of electromagnetic radiation of mobile phones on the central nervous system. Bioelectromagnetics 2003; 24: 49-62.
Çeliker M, Özgür A, Tümkaya L, Terzi S, Yılmaz M, Kalkan Y, Erdoğan E. Effects of exposure to 2100MHz GSM-like radiofrequency electromagnetic field on auditory system of rats. Braz J Otorhinolaryngol 2017; 83: 691-696.
Sonmez OF, Odaci E, Bas O, Kaplan S. Purkinje cell number decreases in the adult female rat cerebellum following exposure to 900 MHz electromagnetic field. Brain Res 2010; 1356: 95- 101.
Valberg PA, van Deventer TE, Repacholi MH. Workgroup report: base stations and wireless networks-radiofrequency (RF) exposures and health consequences. Environ Health Perspect 2007; 115: 416-424.
Christensen HC, Schüz J, Kosteljanetz M, Poulsen HS, Thomsen J, Johansen C. Cellular telephone use and risk of acustic neuroma. Am J Epidemiol 2004; 159: 277-283.
El Gohary MI, Salama AA, El Saeid AA, El Sayed TM, Kotb H. Influence of magnetic field on brain activity during administration of caffeine. Cell Biochem Biophys 2013; 67: 929-933.
Azam S, Hadi N, Khan NU, Hadi SM. Antioxidant and prooxidant properties of caffeine, theobromine and xanthine. Med Sci Monit 2003; 9: BR325-330.
Kolahdouzan M, Hamadeh MJ. The neuroprotective effects of caffeine in neurodegenerative diseases. CNS Neurosci Ther 2017; 23: 272-290.
Tanda G, Goldberg SR. Alteration of the behavioral effects of nicotine by chronic caffeine exposure. Pharmacol Biochem Behav 2000; 66: 47-64.
Devasagayam TPA, Kamat JP, Mohan H, Kesavan PC. Caffeine as an antioxidant: Inhibition of lipid peroxidation induced by reactive oxygen species. BBA-Biomembranes 1996; 1282: 63- 70.
Aoyama K, Matsumura N, Watabe M, Wang F, Kikuchi-Utsumi K, Nakaki T. Caffeine and uric acid mediate glutathione synthesis for neuroprotection. Neuroscience 2011; 181: 206- 215.
Sonsalla PK, Wong LY, Harris SL, Richardson JR, Khobahy I, Li W, Gadad BS, German DC. Delayed caffeine treatment prevents nigral dopamine neuron loss in a progressive rat model of Parkinson’s disease. Exp Neurol 2012; 234: 482-487.
Onger ME, Gocer H, Cirakli A, Buyukceran I, Kilic M, Kaplan S. Effect of electromagnetic wave on bone healing in fixed and unfixed conditions. J Craniofac Surg 2016; 27: 1606-1608.
Hietanen M. Establishing the health risks of exposure to radiofrequency fields requires multidisciplinary research. Scand J Work Environ Health 2006; 32: 169-170.
Manikonda PK, Rajendra P, Devendranath D, Gunasekaran B, Channakeshava, Aradhya RS, Sashidhar RB, Subramanyam C. Influence of extremely low frequency magnetic fields on Ca2+ signaling and NMDA receptor functions in rat hippocampus. Neurosci Lett 2007; 413: 145-149.
Bas O, Odaci E, Kaplan S, Acer N, Ucok K, Colakoglu S. 900 MHz electromagnetic field exposure affects qualitative and quantitative features of hippocampal pyramidal cells in the adult female rat. Brain Res 2009; 1265: 178-185.
Odaci E, Bas O, Kaplan S. Effects of prenatal exposure to a 900 MHz electromagnetic field on the dentate gyrus of rats: a stereological and histopathological study. Brain Res 2008; 1238: 224-229.
Xu SJ, Ning W, Xu ZP, Zhou SY, Chiang H, Luo JH. Chronic exposure to GSM 1800-MHz microwaves reduces excitatory synaptic activity in cultured hippocampal neurons. Neurosci Lett 2006; 398: 253-257.
McEwen BS. The plasticity of the hippocampus is the reason for its vulnerability. Semin Neurosci 1994; 6: 239-246.
Dubreuil D, Jay T, Edeline JM. Head-only exposure to GSM 900-MHz electromagnetic fields does not alter rat’s memory in spatial and non-spatial tasks. Behav Brain Res 2003; 145: 51-61.
Fredholm BB, Battig K, Holmen J, Nehlig A, Zvartau EE. Actions of caffeine in the brain with special reference to factors that contribute to its widespread use. Pharmacol Rev 1999; 51: 83-133.
Singh K, Singh S, Singhal NK, Sharma A, Parmar D, Singh MP. Nicotine- and caffeine-mediated changes in gene expression patterns of MPTP-lesioned mouse striatum: implications in neuroprotection mechanism. Chem Biol Interact 2010; 185: 81-93.
Strong R, Grotta JC, Aronowski J. Combination of low dose ethanol and caffeine protects brain from damage produced by focal ischemia in rats. Neuropharmacology 2000; 39: 515-522.
Fastbom J, Pazos A, Palacios JM. The distribution of adenosine- A1-receptors and 5’-nucleotidase in the brain of some commonly used experimental animals. Neuroscience 1987; 22: 813-826.
Miller LP, Hsu C. Therapeutic potential for adenosine receptor activation in ischemic brain injury. J Neurotraum 1992; 9: S563-S577.
Stavric B, Klassen R, Watkinson B, Karpinski K, Stapley R, Fried P. Variability in caffeine consumption from coffee and tea - possible significance for epidemiological studies. Food Chem Toxicol 1988; 26: 111-118.
Gandhi KK, Williams JM, Menza M, Galazyn M, Benowitz NL. Higher serum caffeine in smokers with schizophrenia compared to smoking controls. Drug Alcohol Depen 2010; 110: 151-155.
Von Lubitz DK, Paul IA, Bartus RT, Jacobson KA. Effects of chronic administration of adenosine A1 receptor agonist and antagonist on spatial learning and memory. Eur J Pharmacol 1993; 249: 271-280.
Johansson B, Georgiev V, Kuosmanen T, Fredholm BB. Long- term treatment with some methylxanthines decreases the susceptibility to bicuculline- and pentylenetetrazol-induced seizures in mice. Relationship to c-fos expression and receptor binding. Eur J Neurosci 1996; 8: 2447-2458.
Luft AR, Skalej M, Schulz JB, Welte D, Kolb R, Bürk K, Klockgether T, Voigt K. Patterns of age-related shrinkage in cerebellum and brainstem observed in vivo using three- dimensional MRI volumetry. Cereb Cortex 1999; 9: 712-721.