The determination of meat species by PCR-RFLP method using mitochondrial ND4 gene in pastırma, a traditional dry cured meat product

Öz Pastırma is a high value, traditional, dry cured, and edible coated meat product. For economic and sociocultural reasons, it is important to know which meat species are used in pastırma. The present study aims to carry out the determination of the meat species in pastırma. A total of 144 pastırma samples were collected from different stores. After genomic DNA isolation, the total DNAs obtained were subjected to polymerase chain reaction (PCR) using specially designed novel primers amplifying the mitochondrial ND4 (MTND4) gene region, specific for cattle (Bos taurus), water buffalo (Bubalus bubalis), horse (Equus caballus), and donkey (Equus asinus) species. For the identification of the meat species, gel purified 952 bp PCR products were digested with fast digest SaqAI restriction enzymes selected based on preliminary in silico analyses. The results of the present study revealed that all of the pastırma samples were made from cattle meat. Cross-reactions and false-positive identifications are serious problems in routine determination of the meat species in food control laboratories. It is important to perform fast, inexpensive determination with high sensitivity and specificity for the identification of meat species. This study has shown that meat species can be detected with high specificity in products such as pastırma, which uses large pieces of meat cuts.

Kaynakça

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Kaynak Göster

Bibtex @araştırma makalesi { tbtkveterinary698691, journal = {Turkish Journal of Veterinary and Animal Sciences}, issn = {1300-0128}, eissn = {1303-6181}, address = {}, publisher = {TÜBİTAK}, year = {2020}, volume = {44}, pages = {35 - 41}, doi = {}, title = {The determination of meat species by PCR-RFLP method using mitochondrial ND4 gene in pastırma, a traditional dry cured meat product}, key = {cite}, author = {Al, Serhat and Hızlısoy, Harun and Ertaş Onmaz, Nurhan and Karadal, Fulden and Güngör, Candan and Yıldırım, Yeliz and Gönülalan, Zafer} }
APA Al, S , Hızlısoy, H , Ertaş Onmaz, N , Karadal, F , Güngör, C , Yıldırım, Y , Gönülalan, Z . (2020). The determination of meat species by PCR-RFLP method using mitochondrial ND4 gene in pastırma, a traditional dry cured meat product . Turkish Journal of Veterinary and Animal Sciences , 44 (1) , 35-41 . Retrieved from https://dergipark.org.tr/tr/pub/tbtkveterinary/issue/52919/698691
MLA Al, S , Hızlısoy, H , Ertaş Onmaz, N , Karadal, F , Güngör, C , Yıldırım, Y , Gönülalan, Z . "The determination of meat species by PCR-RFLP method using mitochondrial ND4 gene in pastırma, a traditional dry cured meat product" . Turkish Journal of Veterinary and Animal Sciences 44 (2020 ): 35-41 <https://dergipark.org.tr/tr/pub/tbtkveterinary/issue/52919/698691>
Chicago Al, S , Hızlısoy, H , Ertaş Onmaz, N , Karadal, F , Güngör, C , Yıldırım, Y , Gönülalan, Z . "The determination of meat species by PCR-RFLP method using mitochondrial ND4 gene in pastırma, a traditional dry cured meat product". Turkish Journal of Veterinary and Animal Sciences 44 (2020 ): 35-41
RIS TY - JOUR T1 - The determination of meat species by PCR-RFLP method using mitochondrial ND4 gene in pastırma, a traditional dry cured meat product AU - Serhat Al , Harun Hızlısoy , Nurhan Ertaş Onmaz , Fulden Karadal , Candan Güngör , Yeliz Yıldırım , Zafer Gönülalan Y1 - 2020 PY - 2020 N1 - DO - T2 - Turkish Journal of Veterinary and Animal Sciences JF - Journal JO - JOR SP - 35 EP - 41 VL - 44 IS - 1 SN - 1300-0128-1303-6181 M3 - UR - Y2 - 2019 ER -
EndNote %0 Turkish Journal of Veterinary and Animal Sciences The determination of meat species by PCR-RFLP method using mitochondrial ND4 gene in pastırma, a traditional dry cured meat product %A Serhat Al , Harun Hızlısoy , Nurhan Ertaş Onmaz , Fulden Karadal , Candan Güngör , Yeliz Yıldırım , Zafer Gönülalan %T The determination of meat species by PCR-RFLP method using mitochondrial ND4 gene in pastırma, a traditional dry cured meat product %D 2020 %J Turkish Journal of Veterinary and Animal Sciences %P 1300-0128-1303-6181 %V 44 %N 1 %R %U
ISNAD Al, Serhat , Hızlısoy, Harun , Ertaş Onmaz, Nurhan , Karadal, Fulden , Güngör, Candan , Yıldırım, Yeliz , Gönülalan, Zafer . "The determination of meat species by PCR-RFLP method using mitochondrial ND4 gene in pastırma, a traditional dry cured meat product". Turkish Journal of Veterinary and Animal Sciences 44 / 1 (Şubat 2020): 35-41 .
AMA Al S , Hızlısoy H , Ertaş Onmaz N , Karadal F , Güngör C , Yıldırım Y , Gönülalan Z . The determination of meat species by PCR-RFLP method using mitochondrial ND4 gene in pastırma, a traditional dry cured meat product. Turkish Journal of Veterinary and Animal Sciences. 2020; 44(1): 35-41.
Vancouver Al S , Hızlısoy H , Ertaş Onmaz N , Karadal F , Güngör C , Yıldırım Y , Gönülalan Z . The determination of meat species by PCR-RFLP method using mitochondrial ND4 gene in pastırma, a traditional dry cured meat product. Turkish Journal of Veterinary and Animal Sciences. 2020; 44(1): 35-41.
IEEE S. Al , H. Hızlısoy , N. Ertaş Onmaz , F. Karadal , C. Güngör , Y. Yıldırım ve Z. Gönülalan , "The determination of meat species by PCR-RFLP method using mitochondrial ND4 gene in pastırma, a traditional dry cured meat product", Turkish Journal of Veterinary and Animal Sciences, c. 44, sayı. 1, ss. 35-41, Şub. 2020

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