Some hematological parameters and enzyme histochemistry of peripheral blood leukocytes in Kivircik sheep

The aim of the present study was to determine some hematological parameters (RBC, WBC, HCT, HGB, MCH, MCHC, MCV, and PLT), the presence and percentages of ANAE and AcP-ase positivities in leukocytes and the differential leukocyte count in Kivircik sheep. Blood samples were taken from the jugular vein of 40 (rams = 20; ewes= 20) healthy, 1-year-old sheep. MCH and MCHC values were found statistically higher in rams than ewes (p < 0.05). Mean percentages of ANAE-positive PBL were defined as 74.16 ± 11.94% in ewes and 70.33 ± 8.39% in rams. It was not determined any significant changes according to sex. On the other hand, the percentages of ACP were determined as 67.12 ± 6.54% in ewes and 66.71 ± 6.58% in rams. It was not found any significant differences according to sex. For ANAE enzyme activity, lymphocytes, monocytes, neutrophils, and eosinophilic granulocytes showed a positive reaction. For ACP enzyme activity, although lymphocytes presented positivity, monocytes, neutrophils, eosinophils were negative. The ratio of lymphocyte, monocyte, neutrophile, eosinophile, and basophile of Kivircik ewes and rams were found as 64.35 ± 5.74%, 60.64 ± 8.85%; 3.41 ± 1.27%, 3.82 ± 1.42%; 27.58 ± 5.46%, 30.94 ± 8.71%; 2.41 ± 0.93%, 2.88 ± 1.45%; 1.76 ± 0.75%, 1.29 ± 0.58%, respectively. The difference between mean values was not found significant according to sex (p > 0.05). In conclusion, our study showed that enzyme histochemical methods can be used for identifying peripheral blood cells and can provide facilitates for early diagnosis of immune system-related disorders in sheep.

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